#
# BioPerl module for Bio::SearchIO::wise
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Jason Stajich <jason-at-bioperl-dot-org>
#
# Copyright Jason Stajich
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::SearchIO::wise - Parsing of wise output as alignments
=head1 SYNOPSIS
use Bio::SearchIO;
my $parser = Bio::SearchIO->new(-file => 'file.genewise',
-format => 'wise',
-wisetype=> 'genewise');
while( my $result = $parser->next_result ) {}
=head1 DESCRIPTION
This object parsers Wise output using Bio::Tools::Genewise or
Bio::Tools::Genomewise as a helper.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
the web:
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHOR - Jason Stajich
Email jason-at-bioperl-dot-org
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::SearchIO::wise;
use vars qw(%MAPPING %MODEMAP $DEFAULT_WRITER_CLASS);
use strict;
# Object preamble - inherits from Bio::Root::Root
use base qw(Bio::SearchIO);
%MODEMAP = ('WiseOutput' => 'result',
'Hit' => 'hit',
'Hsp' => 'hsp'
);
%MAPPING =
(
'Hsp_query-from'=> 'HSP-query_start',
'Hsp_query-to' => 'HSP-query_end',
'Hsp_hit-from' => 'HSP-hit_start',
'Hsp_hit-to' => 'HSP-hit_end',
'Hsp_qseq' => 'HSP-query_seq',
'Hsp_hseq' => 'HSP-hit_seq',
'Hsp_midline' => 'HSP-homology_seq',
'Hsp_score' => 'HSP-score',
'Hsp_qlength' => 'HSP-query_length',
'Hsp_hlength' => 'HSP-hit_length',
'Hsp_align-len' => 'HSP-hsp_length',
'Hsp_positive' => 'HSP-conserved',
'Hsp_identity' => 'HSP-identical',
#'Hsp_gaps' => 'HSP-hsp_gaps',
#'Hsp_hitgaps' => 'HSP-hit_gaps',
#'Hsp_querygaps' => 'HSP-query_gaps',
'Hit_id' => 'HIT-name',
# 'Hit_desc' => 'HIT-description',
# 'Hit_len' => 'HIT-length',
'Hit_score' => 'HIT-score',
'WiseOutput_program' => 'RESULT-algorithm_name',
'WiseOutput_query-def' => 'RESULT-query_name',
'WiseOutput_query-desc'=> 'RESULT-query_description',
'WiseOutput_query-len' => 'RESULT-query_length',
);
$DEFAULT_WRITER_CLASS = 'Bio::SearchIO::Writer::HitTableWriter';
use Bio::Tools::Genewise;
use Bio::Tools::Genomewise;
=head2 new
Title : new
Usage : my $obj = Bio::SearchIO::wise->new();
Function: Builds a new Bio::SearchIO::wise object
Returns : an instance of Bio::SearchIO::wise
Args : -wise => a Bio::Tools::Genewise or Bio::Tools::Genomewise object
=cut
sub _initialize {
my ($self,@args) = @_;
my ( $wisetype, $file,$fh ) =
$self->_rearrange([qw(WISETYPE FILE FH)], @args);
my @newargs;
while( @args ) {
my $a = shift @args;
if( $a =~ /FILE|FH/i ) {
shift @args;
next;
}
push @newargs, $a, shift @args;
}
$self->SUPER::_initialize(@newargs);
# Optimization: caching the EventHandler
# since it's use a lot during the parse.
$self->{'_handler_cache'} = $self->_eventHandler;
$self->wisetype($wisetype);
my @ioargs;
if( $fh ) {
push @ioargs, ('-fh' => $fh);
} elsif( $file ) {
push @ioargs, ('-file' => $file);
}
if( $wisetype =~ /genewise/i ) {
$self->wise(Bio::Tools::Genewise->new(@ioargs));
} elsif( $wisetype =~ /genomewise/i ) {
$self->wise(Bio::Tools::Genomewise->new(@ioargs));
} else {
$self->throw("Must supply a -wisetype to ".ref($self)." which is one of 'genomewise' 'genewise'\n");
}
return $self;
}
=head2 next_result
Title : next_result
Usage : my $hit = $searchio->next_result;
Function: Returns the next Result from a search
Returns : Bio::Search::Result::ResultI object
Args : none
=cut
sub next_result{
my ($self) = @_;
local $/ = "\n";
local $_;
return unless $self->wise;
my $prediction = $self->wise->next_prediction;
return unless $prediction;
$self->{'_reporttype'} = uc $self->wisetype;
$self->start_element({'Name' => 'WiseOutput'});
$self->element({'Name' => 'WiseOutput_program',
'Data' => $self->wisetype});
$self->element({'Name' => 'WiseOutput_query-def',
'Data' => $self->wise->_prot_id});
my @transcripts = $prediction->transcripts;
foreach my $transcript ( @transcripts ) {
my @exons = $transcript->exons;
my $protid;
$self->start_element({'Name' => 'Hit'});
if( $exons[0]->has_tag('supporting_feature') ) {
my ($supporting_feature) = $exons[0]->get_tag_values('supporting_feature');
$protid = $supporting_feature->feature2->seq_id;
$self->element({'Name' => 'Hit_id',
'Data' => $self->wise->_target_id});
}
$self->element({'Name' => 'Hit_score',
'Data' => $self->wise->_score});
foreach my $exon ( @exons ) {
$self->start_element({'Name' => 'Hsp'});
if( $exon->strand < 0 ) {
$self->element({'Name' => 'Hsp_query-from',
'Data' => $exon->end});
$self->element({'Name' => 'Hsp_query-to',
'Data' => $exon->start});
} else {
$self->element({'Name' => 'Hsp_query-from',
'Data' => $exon->start});
$self->element({'Name' => 'Hsp_query-to',
'Data' => $exon->end});
}
$self->element({'Name' => 'Hsp_score',
'Data' => $self->wise->_score});
if( $exon->has_tag('supporting_feature') ) {
my ($sf) = $exon->get_tag_values('supporting_feature');
my $protein = $sf->feature2;
if( $protein->strand < 0 ) {
$self->element({'Name' => 'Hsp_hit-from',
'Data' => $protein->end});
$self->element({'Name' => 'Hsp_hit-to',
'Data' => $protein->start});
} else {
$self->element({'Name' => 'Hsp_hit-from',
'Data' => $protein->start});
$self->element({'Name' => 'Hsp_hit-to',
'Data' => $protein->end});
}
}
$self->element({'Name' => 'Hsp_identity',
'Data' => 0});
$self->element({'Name' => 'Hsp_positive',
'Data' => 0});
$self->end_element({'Name' => 'Hsp'});
}
$self->end_element({'Name' => 'Hit'});
}
$self->end_element({'Name' => 'WiseOutput'});
return $self->end_document();
}
=head2 start_element
Title : start_element
Usage : $eventgenerator->start_element
Function: Handles a start element event
Returns : none
Args : hashref with at least 2 keys 'Data' and 'Name'
=cut
sub start_element{
my ($self,$data) = @_;
# we currently don't care about attributes
my $nm = $data->{'Name'};
my $type = $MODEMAP{$nm};
if( $type ) {
if( $self->_eventHandler->will_handle($type) ) {
my $func = sprintf("start_%s",lc $type);
$self->_eventHandler->$func($data->{'Attributes'});
}
unshift @{$self->{'_elements'}}, $type;
if($type eq 'result') {
$self->{'_values'} = {};
$self->{'_result'}= undef;
}
}
}
=head2 end_element
Title : start_element
Usage : $eventgenerator->end_element
Function: Handles an end element event
Returns : none
Args : hashref with at least 2 keys 'Data' and 'Name'
=cut
sub end_element {
my ($self,$data) = @_;
my $nm = $data->{'Name'};
my $type = $MODEMAP{$nm};
my $rc;
if( $type = $MODEMAP{$nm} ) {
if( $self->_eventHandler->will_handle($type) ) {
my $func = sprintf("end_%s",lc $type);
$rc = $self->_eventHandler->$func($self->{'_reporttype'},
$self->{'_values'});
}
shift @{$self->{'_elements'}};
} elsif( $MAPPING{$nm} ) {
if ( ref($MAPPING{$nm}) =~ /hash/i ) {
my $key = (keys %{$MAPPING{$nm}})[0];
$self->{'_values'}->{$key}->{$MAPPING{$nm}->{$key}} = $self->{'_last_data'};
} else {
$self->{'_values'}->{$MAPPING{$nm}} = $self->{'_last_data'};
}
} else {
$self->debug( "unknown nm $nm, ignoring\n");
}
$self->{'_last_data'} = ''; # remove read data if we are at
# end of an element
$self->{'_result'} = $rc if( defined $type && $type eq 'result' );
return $rc;
}
=head2 element
Title : element
Usage : $eventhandler->element({'Name' => $name, 'Data' => $str});
Function: Convience method that calls start_element, characters, end_element
Returns : none
Args : Hash ref with the keys 'Name' and 'Data'
=cut
sub element{
my ($self,$data) = @_;
$self->start_element($data);
$self->characters($data);
$self->end_element($data);
}
=head2 characters
Title : characters
Usage : $eventgenerator->characters($str)
Function: Send a character events
Returns : none
Args : string
=cut
sub characters{
my ($self,$data) = @_;
return unless ( defined $data->{'Data'} && $data->{'Data'} !~ /^\s+$/ );
$self->{'_last_data'} = $data->{'Data'};
}
=head2 within_element
Title : within_element
Usage : if( $eventgenerator->within_element($element) ) {}
Function: Test if we are within a particular element
This is different than 'in' because within can be tested
for a whole block.
Returns : boolean
Args : string element name
=cut
sub within_element{
my ($self,$name) = @_;
return 0 if ( ! defined $name &&
! defined $self->{'_elements'} ||
scalar @{$self->{'_elements'}} == 0) ;
foreach ( @{$self->{'_elements'}} ) {
if( $_ eq $name ) {
return 1;
}
}
return 0;
}
=head2 in_element
Title : in_element
Usage : if( $eventgenerator->in_element($element) ) {}
Function: Test if we are in a particular element
This is different than 'in' because within can be tested
for a whole block.
Returns : boolean
Args : string element name
=cut
sub in_element{
my ($self,$name) = @_;
return 0 if ! defined $self->{'_elements'}->[0];
return ( $self->{'_elements'}->[0] eq $name)
}
=head2 start_document
Title : start_document
Usage : $eventgenerator->start_document
Function: Handle a start document event
Returns : none
Args : none
=cut
sub start_document{
my ($self) = @_;
$self->{'_lasttype'} = '';
$self->{'_values'} = {};
$self->{'_result'}= undef;
$self->{'_elements'} = [];
$self->{'_reporttype'} = 'exonerate';
}
=head2 end_document
Title : end_document
Usage : $eventgenerator->end_document
Function: Handles an end document event
Returns : Bio::Search::Result::ResultI object
Args : none
=cut
sub end_document{
my ($self,@args) = @_;
return $self->{'_result'};
}
sub write_result {
my ($self, $blast, @args) = @_;
if( not defined($self->writer) ) {
$self->warn("Writer not defined. Using a $DEFAULT_WRITER_CLASS");
$self->writer( $DEFAULT_WRITER_CLASS->new() );
}
$self->SUPER::write_result( $blast, @args );
}
sub result_count {
my $self = shift;
return $self->{'_result_count'};
}
sub report_count { shift->result_count }
=head2 wise
Title : wise
Usage : $obj->wise($newval)
Function: Get/Set the Wise object parser
Returns : value of wise (a scalar)
Args : on set, new value (a scalar or undef, optional)
=cut
sub wise{
my $self = shift;
return $self->{'wise'} = shift if @_;
return $self->{'wise'};
}
=head2 wisetype
Title : wisetype
Usage : $obj->wisetype($newval)
Function: Wise program type
Returns : value of wisetype (a scalar)
Args : on set, new value (a scalar or undef, optional)
=cut
sub wisetype{
my $self = shift;
return $self->{'wisetype'} = shift if @_;
return $self->{'wisetype'};
}
1;