#
# BioPerl module for Bio::Tools::Analysis::Protein::ELM
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Richard Adams <richard.adams@ed.ac.uk>
#
# Copyright Richard Adams
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::Analysis::Protein::ELM - a wrapper around the ELM server which predicts short functional motifs on amino acid sequences
=head1 SYNOPSIS
# get a Bio::Seq object to start with, or a Bio::PrimaryI object.
my $tool = Bio::Tools::Analysis::Protein::ELM->
new(seq => $seqobj->primary_seq() );
$tool->compartment(['ER', 'Golgi']);
$tool->species(9606);
$tool->run;
my @fts = $tool->Result('Bio::SeqFeatureI');
$seqobj->addSeqFeature(@fts);
=head1 DESCRIPTION
This module is a wrapper around the ELM server L<http://elm.eu.org/>
which predicts short functional motifs on amino acid sequences.
False positives can be limited by providing values for the species
and cellular compartment of the protein. To set the species attribute,
use either a L<Bio::Species> object or an NCBI taxon ID number. To set
the cell compartment attribute (any number of compartments can be
chosen) use an array reference to a list of compartment names.
Results can be obtained either as raw text output, parsed into a
data structure, or as Bio::SeqFeature::Generic objects.
=head1 SEE ALSO
L<Bio::SimpleAnalysisI>,
L<Bio::WebAgent>
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHORS
Richard Adams, Richard.Adams@ed.ac.uk,
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
use strict;
package Bio::Tools::Analysis::Protein::ELM;
use vars qw(%cc);
use HTML::HeadParser;
use Bio::SeqFeature::Generic;
use HTTP::Request::Common qw(POST);
use IO::String;
use base qw(Bio::Tools::Analysis::SimpleAnalysisBase);
## valid cell compartments ##
%cc = (
all => 1,
nucleus => 'GO:0005634',
extracellular => 'GO:0005576',
cytoplasm => 'GO:0005737',
peroxisome => 'GO:0005777',
glycosome => 'GO:0020015',
glyoxisome => 'GO:0009514',
golgi => 'GO:0005794',
er => 'GO:0005783',
lysosome => 'GO:0005764',
endosome => 'GO:0005768',
plasma_membrane=> 'GO:0005886',
);
my $URL = 'http://elm.eu.org/cgimodel.py';
my $ANALYSIS_NAME = 'ELM';
my $INPUT_SPEC =
[
{
'mandatory' => 'true',
'type' => 'Bio::PrimarySeqI',
'name' => 'seq',
},
{
'mandatory' => 'false',
'type' => 'taxon_id or Bio::Species object',
'name' => 'species',
'default' => '9606',
},
{
'mandatory' => 'false',
'type' => 'string',
'name' => 'compartment',
'default' => [1],
},
];
my $RESULT_SPEC =
{
'' => 'bulk', # same as undef
'Bio::SeqFeatureI' => 'ARRAY of Bio::SeqFeature::Generic',
'parsed' => '{motif1_name=>{locus=>[],
peptide=>[],
regexp=>[]
},
}',
};
my $ANALYSIS_SPEC= {name => 'ELM',
type => 'Protein',
version => 'n/a',
supplier =>'BioComputing Unit, EMBL',
description =>'Prediction of linear functional motifs
in proteins',
reference => 'NAR, 31:3625-3630'};
sub _init {
my $self = shift;
$self->url($URL);
$self->{'_ANALYSIS_SPEC'} = $ANALYSIS_SPEC;
$self->{'_INPUT_SPEC'} = $INPUT_SPEC;
$self->{'_RESULT_SPEC'} = $RESULT_SPEC;
$self->{'_ANALYSIS_NAME'} = $ANALYSIS_NAME;
return $self;
}
=head2 compartment
name : compartment
usage : $elm->compartment(['golgi', 'er']);
purpose : get/setter for cell compartment specifications
arguments : None, single compartment string or ref to array of
compartment names.
returns : Array of compartment names (default if not previously set).
=cut
sub compartment {
my ($self, $arg) = @_;
if ($arg) {
# convert to array ref if not one already
if (ref ($arg) ne 'ARRAY') {
$arg = [$arg];
}
## now add params if valid
for my $param (@$arg) {
if (exists($cc{lc($param)})) {
push @{$self->{'_compartment'}} , $cc{$param};
} else {
$self->warn("invalid argument ! Must be one of " .
join "\n", keys %cc );
}
} #end of for loop
} #endif $arg
return defined($self->{'_compartment'})? $self->{'_compartment'}
: $self->input_spec()->[2]{'default'};
}
=head1 species
name : species
usage : $tool->species('9606');
purpose : get/setter for species selction for ELM server
arguments : none, taxon_id or Bio::Species object
returns : a string of the ncbi taxon_id
=cut
sub species {
my ($self, $arg) = @_;
if ($arg) {
if (ref($arg) && $arg->isa('Bio::Species')) {
$self->{'_species'} = $arg->ncbi_taxid();
} elsif ($arg =~ /^\d+$/) {
$self->{'_species'} = $arg;
} else {
$self->warn("Argument must be a Bio::Species object or ".
" an integer NCBI taxon id. ");
}
} #end if $arg
return defined($self->{'_species'})?$self->{'_species'}
:$self->input_spec()->[1]{'default'};
}
sub _run {
my $self = shift;
$self->delay(1);
# delay repeated calls by default by 3 sec, set delay() to change
#$self->sleep;
$self->status('TERMINATED_BY_ERROR');
#### this deals with being able to submit multiple checkboxed
#### slections
#1st of all make param array
my @cc_str;
my @cmpts = @{$self->compartment()};
for (my $i = 0; $i <= $#cmpts ; $i++) {
splice @cc_str, @cc_str, 0, 'userCC',$cmpts[$i];
}
my %h = (swissprotId => "",
sequence => $self->seq->seq,
userSpecies => $self->species,
typedUserSpecies => '',
fun => "Submit");
splice (@cc_str, @cc_str,0, ( map{$_, $h{$_}} keys %h));
my $request = POST $self->url(),
Content_Type => 'form-data',
Content => \@cc_str;
$self->debug( $request->as_string);
my $r1 = $self->request($request);
if ( $r1->is_error ) {
$self->warn(ref($self)." Request Error:\n".$r1->as_string);
return;
}
my $text = $r1->content;
my ($url) = $text =~ /URL=\S+(fun=\S+r=\d)/s;
#$url =~ s/amp;//g ;
my ($resp2);
$url = $URL . "?" .$url;
while (1) {
my $req2 = HTTP::Request->new(GET=>$url);
my $r2 = $self->request ($req2);
if ( $r2->is_error ) {
$self->warn(ref($self)." Request Error:\n".$r2->as_string);
return;
}
$resp2 = $r2->content();
if ($resp2 !~ /patient/s) {
$self->status('COMPLETED');
$resp2=~ s/<[^>]+>/ /sg;
$self->{'_result'} = $resp2;
return;
} else {
print "." if $self->verbose > 0;
$self->sleep(1);
}
}
}
=head1 result
name : result
usage : $tool->result('Bio::SeqFeatureI');
purpose : parse results into sequence features or basic data format
arguments : 1. none (retrieves raw text without html)
2. a value (retrieves data structure)
3. 'Bio::SeqFeatureI' (returns array of sequence features)
tag names are : {method => 'ELM', motif => motifname,
peptide => seqeunce of match,
concensus => regexp of match}.
returns : see arguments.
=cut
sub result {
my ($self, $val) = @_;
if ($val) {
if (!exists($self->{'_parsed'}) ) {
$self->_parse_raw();
}
if ($val eq 'Bio::SeqFeatureI') {
my @fts;
for my $motif (keys %{$self->{'_parsed'}}) {
for (my $i = 0; $i< scalar @{$self->{'_parsed'}{$motif}{'locus'}};$i++) {
my ($st, $end) = split /\-/, $self->{'_parsed'}{$motif}{'locus'}[$i];
push @fts, Bio::SeqFeature::Generic->new
(
-start => $st,
-end => $end,
-primary_tag => 'Domain',
-source => 'ELM',
-tag => {
method => 'ELM',
motif => $motif,
peptide => $self->{'_parsed'}{$motif}{'peptide'}[$i],
concensus => $self->{'_parsed'}{$motif}{'regexp'}[0],
});
}
}
return @fts;
} #end if BioSeqFeature
return $self->{'_parsed'};
} #endif ($val)
return $self->{'_result'};
}
## internal sub to parse raw data into internal data structure which is cached.
sub _parse_raw {
my $self = shift;
my $result = IO::String->new($self->{'_result'});
my $in_results = 0;
my $name;
my %results;
my $last;
while (my $l = <$result>) {
next unless $in_results > 0 ||$l =~ /^\s+Elm\s+Name\s+Instances/;
$in_results++; #will be set whnstart of results reached.
last if $l =~ /List of excluded/;
next unless $in_results >1;
my @line_parts = split /\s+/, $l;
shift @line_parts;
## if result has motif name on 1 line
if (scalar @line_parts == 1 && $line_parts[0]=~ /^\s*(\w+_\w+)/) {
$name = $1;
next;
}
## else if is line with loci /seq matches
elsif (@line_parts > 1) {
my $index = 0; ## array index
my $read_loci = 0; ## flag to know that loci are being read
while ($index <= $#line_parts) {
my $word = $line_parts[$index++];
if ($read_loci ==0 && $word =~/_/) {
$name = $word;
} elsif ($read_loci == 0 && $word =~ /^\w+$/ ) {
push @{$results{$name}{'peptide'}}, $word;
} elsif ($word =~ /\d+\-\d+/) {
$read_loci = 1;
push @{$results{$name}{'locus'}}, $word;
} else { ## only get here if there are elements
last;
}
} #end of while
push @{$results{$name}{'regexp'}}, $line_parts[$#line_parts];
} #end of elsif
} #end of while
$self->{'_parsed'} = \%results;
}
1;