#
# BioPerl module for Bio::Assembly::Contig
# Mostly based on Bio::SimpleAlign by Ewan Birney
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Robson Francisco de Souza <rfsouza@citri.iq.usp.br>
#
# Copyright Robson Francisco de Souza
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Assembly::Contig - Perl module to hold and manipulate
sequence assembly contigs.
=head1 SYNOPSIS
# Module loading
use Bio::Assembly::IO;
# Assembly loading methods
$aio = Bio::Assembly::IO->new(-file=>"test.ace.1",
-format=>'phrap');
$assembly = $aio->next_assembly;
foreach $contig ($assembly->all_contigs) {
# do something
}
# OR, if you want to build the contig yourself,
use Bio::Assembly::Contig;
$c = Bio::Assembly::Contig->new(-id=>"1");
$ls = Bio::LocatableSeq->new(-seq=>"ACCG-T",
-id=>"r1",
-alphabet=>'dna');
$ls2 = Bio::LocatableSeq->new(-seq=>"ACA-CG-T",
-id=>"r2",
-alphabet=>'dna');
$ls_coord = Bio::SeqFeature::Generic->new(-start=>3,
-end=>8,
-strand=>1);
$ls2_coord = Bio::SeqFeature::Generic->new(-start=>1,
-end=>8,
-strand=>1);
$c->add_seq($ls);
$c->add_seq($ls2);
$c->set_seq_coord($ls_coord,$ls);
$c->set_seq_coord($ls2_coord,$ls2);
$con = Bio::LocatableSeq->new(-seq=>"ACACCG-T",
-alphabet=>'dna');
$c->set_consensus_sequence($con);
$l = $c->change_coord('unaligned r2','ungapped consensus',6);
print "6 in unaligned r2 => $l in ungapped consensus\n";
=head1 DESCRIPTION
A contig is as a set of sequences, locally aligned to each other, so
that every sequence has overlapping regions with at least one sequence
in the contig, such that a continuous of overlapping sequences is
formed, allowing the deduction of a consensus sequence which may be
longer than any of the sequences from which it was deduced.
In this documentation we refer to the overlapping sequences used to
build the contig as "aligned sequences" and to the sequence deduced
from the overlap of aligned sequences as the "consensus". Methods to
deduce the consensus sequence from aligned sequences were not yet
implemented in this module, but its posssible to add a consensus
sequence deduced by other means, e.g, by the assembly program used to
build the alignment.
All aligned sequences in a Bio::Assembly::Contig must be Bio::Assembly::Locatable
objects and have a unique ID. The unique ID restriction is due to the
nature of the module's internal data structures and is also a request
of some assembly programs. If two sequences with the same ID are added
to a contig, the first sequence added is replaced by the second one.
=head2 Coordinate_systems
There are four base coordinate systems in Bio::Assembly::Contig. When
you need to access contig elements or data that exists on a certain
range or location, you may be specifying coordinates in relation to
different sequences, which may be either the contig consensus or one
of the aligned sequences that were used to do the assembly.
=========================================================
Name | Referenced sequence
---------------------------------------------------------
"gapped consensus" | Contig (with gaps)
"ungapped consensus" | Contig (without gaps)
"aligned $seqID" | sequence $seqID (with gaps)
"unaligned $seqID" | sequence $seqID (without gaps)
=========================================================
"gapped consensus" refers to positions in the aligned consensus
sequence, which is the consensus sequence including the gaps inserted
to align it agains the aligned sequences that were used to assemble
the contig. So, its limits are [ 1, (consensus length + number of gaps
in consensus) ]
"ungapped consensus" is a coordinate system based on the consensus
sequence, but excluding consensus gaps. This is just the coordinate
system that you have when considering the consensus sequence alone,
instead of aligned to other sequences.
"aligned $seqID" refers to locations in the sequence $seqID after
alignment of $seqID against the consensus sequence (reverse
complementing the original sequence, if needed). Coordinate 1 in
"aligned $seqID" is equivalent to the start location (first base) of
$seqID in the consensus sequence, just like if the aligned sequence
$seqID was a feature of the consensus sequence.
"unaligned $seqID" is equivalent to a location in the isolated
sequence, just like you would have when considering the sequence
alone, out of an alignment. When changing coordinates from "aligned
$seq2" to "unaligned $seq2", if $seq2 was reverse complemented when
included in the alignment, the output coordinates will be reversed to
fit that fact, i.e. 1 will be changed to length($seq2), 2 will be
length($seq)-1 and so on.
An important note: when you change gap coordinates from a gapped
system ("gapped consensus" or "aligned $seqID") to a system that does
not include gaps ("ungapped consensus" or "unaligned $seqID"), the
position returned will be the first location before all gaps
neighboring the input location.
=head2 Feature_collection
Bio::Assembly::Contig stores much information about a contig in a
Bio::Assembly::SeqFeature::Collection object. Relevant information on the
alignment is accessed by selecting features based on their primary
tags (e.g. all features which have a primary tag of the form
'_aligned_coord:$seqID', where $seqID is an aligned sequence ID, are
coordinates for sequences in the contig alignment) and, by using
methods from Bio::Assembly::SeqFeature::Collection, it's possible to select
features by overlap with other features.
We suggest that you use the primary tags of features as identifiers
for feature classes. By convention, features with primary tags
starting with a '_' are generated by modules that populate the contig
data structure and return the contig object, maybe as part of an
assembly object, e.g. drivers from the Bio::Assembly::IO set.
Features in the features collection may be associated with particular
aligned sequences. To obtain this, you must attach the sequence to the
feature, using attach() seq from Bio::Assembly::SeqFeatureI, before you add the
feature to the feature collection. We also suggest to add the sequence
id to the primary tag, so that is easy to select feature for a
particular sequence.
There is only one feature class that some methods in
Bio::Assembly::Contig expect to find in the feature collection: features
with primary tags of the form '_aligned_coord:$seqID', where $seqID is
the aligned sequence id (like returned by $seq-E<gt>id()). These features
describe the position (in "gapped consensus" coordinates) of aligned
sequences, and the method set_seq_coord() automatically changes a
feature's primary tag to this form whenever the feature is added to
the collection by this method. Only two methods in Bio::Assembly::Contig
will not work unless there are features from this class:
change_coord() and get_seq_coord().
Other feature classes will be automatically available only when
Bio::Assembly::Contig objects are created by a specific module. Such
feature classes are (or should be) documented in the documentation of
the module which create them, to which the user should refer.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to the
Bioperl mailing lists Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHOR - Robson Francisco de Souza
rfsouza@citri.iq.usp.br
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
#'
package Bio::Assembly::Contig;
use strict;
use Bio::DB::SeqFeature::Store; # isa Bio::SeqFeature::CollectionI
use Bio::Seq::PrimaryQual; # isa Bio::Seq::QualI
use Scalar::Util qw(weaken);
use base qw(Bio::Root::Root Bio::Align::AlignI);
=head1 Object creator
=head2 new
Title : new
Usage : my $contig = Bio::Assembly::Contig->new();
Function : Creates a new contig object
Returns : Bio::Assembly::Contig
Args : -id => unique contig ID
-source => string for the sequence assembly program used
-collection => Bio::SeqFeature::CollectionI instance
=cut
#-----------
sub new {
#-----------
my ($class, @args) = @_;
my $self = $class->SUPER::new(@args);
my ($src, $id, $collection) = $self->_rearrange([qw(SOURCE ID COLLECTION)], @args);
$src && $self->source($src);
($id && $self->id($id)) || ($self->{'_id'} = 'NoName'); # Alignment (contig) name
($id && $self->id($id)) || ($self->{'_source'} = 'Unknown'); # Program used to build the contig
# we need to set up internal hashes first!
# Bio::SimpleAlign derived fields (check which ones are needed for AlignI compatibility)
$self->{'_elem'} = {}; # contig elements: aligned sequence objects (keyed by ID)
$self->{'_order'} = {}; # store sequence order
# $self->{'start_end_lists'} = {}; # References to entries in {'_seq'}. Keyed by seq ids.
# $self->{'_dis_name'} = {}; # Display names for each sequence
$self->{'_symbols'} = {}; # List of symbols
#Contig specific slots
$self->{'_consensus_sequence'} = undef;
$self->{'_consensus_quality'} = undef;
$self->{'_nof_residues'} = 0;
$self->{'_nof_seqs'} = 0;
# $self->{'_nof_segments'} = 0; # Let's not make it heavier than needed by now...
# for cases where SF::Collection is shared between Bio::Assembly::Contig
if ($collection) {
$self->throw("Collection must implement Bio::SeqFeature::CollectionI") unless $collection->isa('Bio::SeqFeature::CollectionI');
$self->{'_sfc'} = $collection;
} else {
$self->{'_sfc'} = Bio::DB::SeqFeature::Store->new(
-adaptor => 'memory',
-index_subfeatures => 1,
);
}
# Assembly specifics
$self->{'_assembly'} = undef; # Bio::Assembly::Scaffold the contig belongs to
$self->{'_strand'} = 0; # Reverse (-1) or forward (1), if contig is in a scaffold. 0 otherwise
$self->{'_neighbor_start'} = undef; # Neighbor Bio::Assembly::Contig
$self->{'_neighbor_end'} = undef; # Neighbor Bio::Assembly::Contig
return $self; # success - we hope!
}
=head1 Assembly related methods
These methods exist to enable adding information about possible
relations among contigs, e.g. when you already have a scaffold for
your assembly, describing the ordering of contigs in the final
assembly, but no sequences covering the gaps between neighboring
contigs.
=head2 source
Title : source
Usage : $contig->source($program);
Function : Get/Set program used to build this contig
Returns : string
Argument : [optional] string
=cut
sub source {
my $self = shift;
my $source = shift;
$self->{'_source'} = $source if (defined $source);
return $self->{'_source'};
}
=head2 assembly
Title : assembly
Usage : $contig->assembly($assembly);
Function : Get/Set assembly object for this contig
Returns : a Bio::Assembly::Scaffold object
Argument : a Bio::Assembly::Scaffold object
=cut
sub assembly {
my $self = shift;
my $assembly = shift;
$self->throw("Using non Bio::Assembly::Scaffold object when assign contig to assembly")
if (defined $assembly && ! $assembly->isa("Bio::Assembly::Scaffold"));
# We create a circular reference to a Scaffold object. It is made weak
# to prevent memory leaks.
$self->{'_assembly'} = $assembly if (defined $assembly);
weaken($self->{'_assembly'});
return $self->{'_assembly'};
}
=head2 strand
Title : strand
Usage : $contig->strand($num);
Function : Get/Set contig orientation in a scaffold/assembly.
Its equivalent to the strand property of sequence
objects and sets whether the contig consensus should
be reversed and complemented before being added to a
scaffold or assembly.
Returns : integer
Argument : 1 if orientaion is forward, -1 if reverse and
0 if none
=cut
sub strand {
my $self = shift;
my $ori = shift;
if (defined $ori) {
$self->throw("Contig strand must be either 1, -1 or 0")
unless $ori == 1 || $ori == 0 || $ori == -1;
$self->{'_strand'} = $ori;
}
return $self->{'_strand'};
}
=head2 upstream_neighbor
Title : upstream_neighbor
Usage : $contig->upstream_neighbor($contig);
Function : Get/Set a contig neighbor for the current contig when
building a scaffold. The upstream neighbor is
located before $contig first base
Returns : nothing
Argument : Bio::Assembly::Contig
=cut
sub upstream_neighbor {
my $self = shift;
my $ref = shift;
$self->throw("Trying to assign a non Bio::Assembly::Contig object to upstream contig")
if (defined $ref && ! $ref->isa("Bio::Assembly::Contig"));
$self->{'_neighbor_start'} = $ref if (defined $ref);
return $self->{'_neighbor_start'};
}
=head2 downstream_neighbor
Title : downstream_neighbor
Usage : $contig->downstream_neighbor($num);
Function : Get/Set a contig neighbor for the current contig when
building a scaffold. The downstream neighbor is
located after $contig last base
Returns : nothing
Argument : Bio::Assembly::Contig
=cut
sub downstream_neighbor {
my $self = shift;
my $ref = shift;
$self->throw("Trying to assign a non Bio::Assembly::Contig object to downstream contig")
if (defined $ref && ! $ref->isa("Bio::Assembly::Contig"));
$self->{'_neighbor_end'} = $ref if (defined $ref);
return $self->{'_neighbor_end'};
}
=head1 Contig feature collection methods
=head2 add_features
Title : add_features
Usage : $contig->add_features($feat,$flag)
Function :
Add an array of features to the contig feature
collection. The consensus sequence may be attached to the
added feature, if $flag is set to 1. If $flag is 0 and
the feature attached to one of the contig aligned
sequences, the feature is registered as an aligned
sequence feature. If $flag is 0 and the feature is not
attched to any sequence in the contig, the feature is
simply added to the feature collection and no attachment
or registration is made.
Note: You must attach aligned sequences to their features
prior to calling add_features, otherwise you won't be
able to access the feature through get_seq_feat_by_tag()
method.
Returns : number of features added.
Argument :
$feat : A reference to an array of Bio::SeqFeatureI
$flag : boolean - true if consensus sequence object
should be attached to this feature, false if
no consensus attachment should be made.
Default: false.
=cut
sub add_features {
my ($self, $args, $flag) = @_;
# Adding shortcuts for aligned sequence features
$flag = 0 unless (defined $flag);
if ($flag && defined $self->{'_consensus_sequence'}) {
foreach my $feat (@$args) {
next if (defined $feat->seq);
$feat->attach_seq($self->{'_consensus_sequence'});
}
} elsif (!$flag) { # Register aligned sequence features
foreach my $feat (@$args) {
if (my $seq = $feat->entire_seq()) {
my $seqID = $seq->id() || $seq->display_id || $seq->primary_id;
$self->warn("Adding contig feature attached to unknown sequence $seqID!")
unless (exists $self->{'_elem'}{$seqID});
my $tag = $feat->primary_tag;
$self->{'_elem'}{$seqID}{'_feat'}{$tag} = $feat;
}
}
}
# Add feature to feature collection
my $nof_added = $self->get_features_collection->add_features($args);
return $nof_added;
}
=head2 remove_features
Title : remove_features
Usage : $contig->remove_features(@feat)
Function : Remove an array of contig features
Returns : true if successful
Argument : An array of Bio::SeqFeature::Generic (Bio::SeqFeatureI)
=cut
sub remove_features {
my ($self, @args) = @_;
# Removing shortcuts for aligned sequence features
for my $feat (@args) {
if (my $seq = $feat->entire_seq()) {
my $seqID = $seq->id || $seq->display_id || $seq->primary_id;
my $tag = $feat->primary_tag;
$tag =~ s/:$seqID$/$1/g;
delete( $self->{'_elem'}{$seqID}{'_feat'}{$tag} )
if (exists $self->{'_elem'}{$seqID}{'_feat'}{$tag} &&
$self->{'_elem'}{$seqID}{'_feat'}{$tag} eq $feat);
}
}
# Removing Bio::SeqFeature objects
return $self->get_features_collection->delete(@args);
}
=head2 get_features_collection
Title : get_features_collection
Usage : $contig->get_features_collection()
Function : Get the collection of all contig features and seqfeatures
Returns : Bio::DB::SeqFeature::Store (Bio::SeqFeature::CollectionI)
Argument : none
=cut
sub get_features_collection {
my $self = shift;
return $self->{'_sfc'};
}
=head2 remove_features_collection
Title : remove_features_collection
Usage : $contig->remove_features_collection()
Function : Remove the collection of all contig features. It is useful
to save some memory (when contig features are not needed).
Returns : none
Argument : none
=cut
sub remove_features_collection {
my $self = shift;
# Removing shortcuts for aligned sequence features
for my $seqID (keys %{$self->{'_elem'}}) {
delete $self->{'_elem'}{$seqID};
}
# Removing Bio::SeqFeature::Collection features
$self->{'_sfc'} = {};
return;
}
=head1 Coordinate system's related methods
See L<Coordinate_Systems> above.
=head2 change_coord
Title : change_coord
Usage : $contig->change_coord($in,$out,$query)
Function :
Change coordinate system for $query. This method
transforms locations between coordinate systems described
in section "Coordinate Systems" of this document.
Note: this method will throw an exception when changing
coordinates between "ungapped consensus" and other
systems if consensus sequence was not set. It will also
throw exceptions when changing coordinates among aligned
sequence, either with or without gaps, and other systems
if sequence locations were not set with set_seq_coord().
Returns : integer
Argument :
$in : [string] input coordinate system
$out : [string] output coordinate system
$query : [integer] a position in a sequence
=cut
sub change_coord {
my $self = shift;
my $type_in = shift;
my $type_out = shift;
my $query = shift;
# Parsing arguments
# Loading read objects (these calls will throw exceptions whether $read_in or
# $read_out is not found
my ($read_in,$read_out) = (undef,undef);
my $in_ID = ( split(' ',$type_in) )[1];
my $out_ID = ( split(' ',$type_out) )[1];
if ($in_ID ne 'consensus') {
$read_in = $self->get_seq_coord( $self->get_seq_by_name($in_ID) );
$self->throw("Can't change coordinates without sequence location for $in_ID")
unless (defined $read_in);
}
if ($out_ID ne 'consensus') {
$read_out = $self->get_seq_coord( $self->get_seq_by_name($out_ID) );
$self->throw("Can't change coordinates without sequence location for $out_ID")
unless (defined $read_out);
}
# Performing transformation between coordinates
SWITCH1: {
# Transformations between contig padded and contig unpadded
(($type_in eq 'gapped consensus') && ($type_out eq 'ungapped consensus')) && do {
$self->throw("Can't use ungapped consensus coordinates without a consensus sequence")
unless (defined $self->{'_consensus_sequence'});
$query = &_padded_unpadded($self->{'_consensus_gaps'}, $query);
last SWITCH1;
};
(($type_in eq 'ungapped consensus') && ($type_out eq 'gapped consensus')) && do {
$self->throw("Can't use ungapped consensus coordinates without a consensus sequence")
unless (defined $self->{'_consensus_sequence'});
$query = &_unpadded_padded($self->{'_consensus_gaps'},$query);
last SWITCH1;
};
# Transformations between contig (padded) and read (padded)
(($type_in eq 'gapped consensus') &&
($type_out =~ /^aligned /) && defined($read_out)) && do {
$query = $query - $read_out->start() + 1;
last SWITCH1;
};
(($type_in =~ /^aligned /) && defined($read_in) &&
($type_out eq 'gapped consensus')) && do {
$query = $query + $read_in->start() - 1;
last SWITCH1;
};
# Transformations between contig (unpadded) and read (padded)
(($type_in eq 'ungapped consensus') &&
($type_out =~ /^aligned /) && defined($read_out)) && do {
$query = $self->change_coord('ungapped consensus','gapped consensus',$query);
$query = $self->change_coord('gapped consensus',"aligned $out_ID",$query);
last SWITCH1;
};
(($type_in =~ /^aligned /) && defined($read_in) &&
($type_out eq 'ungapped consensus')) && do {
$query = $self->change_coord("aligned $in_ID",'gapped consensus',$query);
$query = $self->change_coord('gapped consensus','ungapped consensus',$query);
last SWITCH1;
};
# Transformations between seq $read_in padded and seq $read_out padded
(defined($read_in) && ($type_in =~ /^aligned /) &&
defined($read_out) && ($type_out =~ /^aligned /)) && do {
$query = $self->change_coord("aligned $in_ID",'gapped consensus',$query);
$query = $self->change_coord('gapped consensus',"aligned $out_ID",$query);
last SWITCH1;
};
# Transformations between seq $read_in padded and seq $read_out unpadded
(defined($read_in) && ($type_in =~ /^aligned /) &&
defined($read_out) && ($type_out =~ /^unaligned /)) && do {
if ($read_in ne $read_out) {
$query = $self->change_coord("aligned $in_ID",'gapped consensus',$query);
$query = $self->change_coord('gapped consensus',"aligned $out_ID",$query);
}
my $list_out = $self->{'_elem'}{$out_ID}{'_gaps'};
$query = &_padded_unpadded($list_out,$query);
# Changing read orientation if read was reverse complemented when aligned
if ($read_out->strand == -1) {
my ($length) = $read_out->length();
$length = $length - &_nof_gaps($list_out,$length);
$query = $length - $query + 1;
}
last SWITCH1;
};
(defined($read_in) && ($type_in =~ /^unaligned /) &&
defined($read_out) && ($type_out =~ /^aligned /)) && do {
my $list_in = $self->{'_elem'}{$in_ID}{'_gaps'};
# Changing read orientation if read was reverse complemented when aligned
if ($read_in->strand == -1) {
my ($length) = $read_in->length();
$length = $length - &_nof_gaps($list_in,$length);
$query = $length - $query + 1;
}
$query = &_unpadded_padded($list_in,$query);
if ($read_in ne $read_out) {
$query = $self->change_coord("aligned $in_ID",'gapped consensus',$query);
$query = $self->change_coord('gapped consensus',"aligned $out_ID",$query);
}
last SWITCH1;
};
# Transformations between seq $read_in unpadded and seq $read_out unpadded
(defined($read_in) && ($type_in =~ /^unaligned /) &&
defined($read_out) && ($type_out =~ /^unaligned /)) && do {
$query = $self->change_coord("unaligned $in_ID","aligned $out_ID",$query);
$query = $self->change_coord("aligned $out_ID","unaligned $out_ID",$query);
last SWITCH1;
};
# Transformations between contig (padded) and read (unpadded)
(($type_in eq 'gapped consensus') &&
($type_out =~ /^unaligned /) && defined($read_out)) && do {
$query = $self->change_coord('gapped consensus',"aligned $out_ID",$query);
$query = $self->change_coord("aligned $out_ID","unaligned $out_ID",$query);
last SWITCH1;
};
(($type_in =~ /^unaligned /) && defined($read_in) &&
($type_out eq 'gapped consensus')) && do {
$query = $self->change_coord("unaligned $in_ID","aligned $in_ID",$query);
$query = $self->change_coord("aligned $in_ID",'gapped consensus',$query);
last SWITCH1;
};
# Transformations between contig (unpadded) and read (unpadded)
(($type_in eq 'ungapped consensus') &&
($type_out =~ /^unaligned /) && defined($read_out)) && do {
$query = $self->change_coord('ungapped consensus','gapped consensus',$query);
$query = $self->change_coord('gapped consensus',"unaligned $out_ID",$query);
last SWITCH1;
};
(($type_in =~ /^unaligned /) && defined($read_in) &&
($type_out eq 'ungapped consensus')) && do {
$query = $self->change_coord("unaligned $in_ID",'gapped consensus',$query);
$query = $self->change_coord('gapped consensus','ungapped consensus',$query);
last SWITCH1;
};
$self->throw("Unknow coordinate system. Args: $type_in, $type_out.");
$query = undef; # If a coordinate systems just requested is unknown
}
return $query;
}
=head2 get_seq_coord
Title : get_seq_coord
Usage : $contig->get_seq_coord($seq);
Function : Get "gapped consensus" location for aligned sequence
Returns : Bio::SeqFeature::Generic for coordinates or undef.
A warning is printed if sequence coordinates were not set.
Argument : Bio::LocatableSeq object
=cut
sub get_seq_coord {
my ($self,$seq) = @_;
if( !ref $seq || ! $seq->isa('Bio::LocatableSeq') ) {
$self->throw("$seq is not a Bio::LocatableSeq");
}
my $seqID = $seq->id() || $seq->display_id || $seq->primary_id;
unless (exists( $self->{'_elem'}{$seqID} )) {
$self->warn("No such sequence ($seqID) in contig ".$self->id);
return;
}
unless (exists( $self->{'_elem'}{$seqID}{'_feat'}{"_aligned_coord:$seqID"} )) {
# $self->warn("Chad. Location not set for sequence ($seqID) in contig ".$self->id);
return;
}
return $self->{'_elem'}{$seqID}{'_feat'}{"_aligned_coord:$seqID"};
}
=head2 set_seq_coord
Title : set_seq_coord
Usage : $contig->set_seq_coord($feat,$seq);
Function :
Set "gapped consensus" location for an aligned
sequence. If the sequence was previously added using
add_seq, its coordinates are changed/set. Otherwise,
add_seq is called and the sequence is added to the
contig.
Returns : Bio::SeqFeature::Generic for old coordinates or undef.
Argument :
$feat : a Bio::SeqFeature::Generic object
representing a location for the
aligned sequence, in "gapped
consensus" coordinates.
Note: the original feature primary tag will
be lost.
$seq : a Bio::LocatableSeq object
=cut
sub set_seq_coord {
my ($self,$feat,$seq) = @_;
if( !ref $seq || ! $seq->isa('Bio::LocatableSeq') ) {
$self->throw("Unable to process non locatable sequences [".ref($seq)."]");
}
# Complaining about inadequate feature object
$self->throw("Coordinates must be a Bio::SeqFeature::Generic object!")
unless ( $feat->isa("Bio::SeqFeature::Generic") );
$self->throw("Sequence coordinates must have an end!")
unless (defined $feat->end);
$self->throw("Sequence coordinates must have a start!")
unless (defined $feat->start);
my $seqID = $seq->id() || $seq->display_id || $seq->primary_id;
if ( exists( $self->{'_elem'}{$seqID} ) &&
exists( $self->{'_elem'}{$seqID}{'_seq'} ) &&
defined( $self->{'_elem'}{$seqID}{'_seq'} ) &&
($seq ne $self->{'_elem'}{$seqID}{'_seq'}) ) {
$self->warn("Replacing sequence $seqID\n");
$self->remove_seq($self->{'_elem'}{$seqID}{'_seq'});
$self->remove_features($feat);
}
# Add new sequence and Bio::Generic::SeqFeature
$self->add_seq($seq);
$feat->add_tag_value('contig',$self->id) unless ( $feat->has_tag('contig') );
$feat->primary_tag("_aligned_coord");
$feat->source_tag($seqID);
$feat->attach_seq($seq);
$self->{'_elem'}{$seqID}{'_feat'}{"_aligned_coord:$seqID"} = $feat;
$self->add_features([ $feat ]);
}
=head1 Bio::Assembly::Contig consensus methods
=head2 set_consensus_sequence
Title : set_consensus_sequence
Usage : $contig->set_consensus_sequence($seq)
Function : Set the consensus sequence object for this contig
Returns : consensus length
Argument : Bio::LocatableSeq
=cut
sub set_consensus_sequence {
my $self = shift;
my $seq = shift;
$self->throw("Consensus sequence must be a Bio::LocatableSeq!")
unless ($seq->isa("Bio::LocatableSeq"));
$self->{'_consensus_gaps'} = []; # Consensus Gap registry
$self->_register_gaps( $seq->seq, $self->{'_consensus_gaps'} );
$self->{'_consensus_sequence'} = $seq;
$seq->start(1);
$seq->end($seq->_ungapped_len);
my $con_len = $seq->length;
return $con_len;
}
=head2 set_consensus_quality
Title : set_consensus_quality
Usage : $contig->set_consensus_quality($qual)
Function : Set the quality object for consensus sequence
Returns : nothing
Argument : Bio::Seq::QualI object
=cut
sub set_consensus_quality {
my ($self, $qual) = @_;
$self->throw("Consensus quality must be a Bio::Seq::QualI object!")
unless ( $qual->isa("Bio::Seq::QualI") );
$self->throw("Consensus quality can't be added before you set the consensus sequence!")
unless (defined $self->{'_consensus_sequence'});
$self->{'_consensus_quality'} = $qual;
}
=head2 get_consensus_length
Title : get_consensus_length
Usage : $contig->get_consensus_length()
Function : Get consensus sequence length
Returns : integer
Argument : none
=cut
sub get_consensus_length {
my $self = shift;
return $self->{'_consensus_sequence'}->length();
}
=head2 get_consensus_sequence
Title : get_consensus_sequence
Usage : $contig->get_consensus_sequence()
Function : Get a reference to the consensus sequence object
for this contig
Returns : Bio::SeqI object
Argument : none
=cut
sub get_consensus_sequence {
my ($self, @args) = @_;
return $self->{'_consensus_sequence'};
}
=head2 get_consensus_quality
Title : get_consensus_quality
Usage : $contig->get_consensus_quality()
Function : Get a reference to the consensus quality object
for this contig.
Returns : A Bio::Seq::QualI object
Argument : none
=cut
sub get_consensus_quality {
my ($self, @args) = @_;
return $self->{'_consensus_quality'};
}
=head1 Bio::Assembly::Contig aligned sequences methods
=head2 set_seq_qual
Title : set_seq_qual
Usage : $contig->set_seq_qual($seq,$qual);
Function : Adds quality to an aligned sequence.
Returns : nothing
Argument : a Bio::LocatableSeq object and
a Bio::Seq::QualI object
See L<Bio::LocatableSeq> for more information.
=cut
sub set_seq_qual {
my ($self,$seq,$qual) = @_;
if( !ref $seq || ! $seq->isa('Bio::LocatableSeq') ) {
$self->throw("Unable to process non locatable sequences [".ref($seq)."]");
}
my $seqID = $seq->id() || $seq->display_id || $seq->primary_id;
$self->throw("Consensus quality must be a Bio::Seq::QualI object!")
unless ( $qual->isa("Bio::Seq::QualI") );
$self->throw("Use add_seq first: aligned sequence qualities can't be added before you load the sequence!")
unless (exists $self->{'_elem'}{$seqID}{'_seq'});
$self->throw("Use set_seq_coord first: aligned sequence qualities can't be added before you add coordinates for the sequence!") unless (defined( $self->get_seq_coord($seq) ));
# Adding gaps to quality object
my $sequence = $self->{'_elem'}{$seqID}{'_seq'}->seq();
my $tmp = $qual->qual();
@{$tmp} = reverse(@{$tmp}) if ($self->get_seq_coord($seq)->strand() == -1);
my @quality = ();
my $previous = 0;
my $next = 0;
my $i = 0; my $j = 0;
while ($i <= $#{$tmp}) {
# IF base is a gap, quality is the average for neighbouring sites
if ($j > $i && substr($sequence,$j,1) eq '-') {
$previous = $tmp->[$i-1] unless ($i == 0);
if ($i < $#{$tmp}) {
$next = $tmp->[$i+1];
} else {
$next = 0;
}
push(@quality,int( ($previous+$next)/2 ));
} else {
push(@quality,$tmp->[$i]);
$i++;
}
$j++;
}
$self->{'_elem'}{$seqID}{'_qual'} = Bio::Seq::PrimaryQual->new(
-qual=>join(" ",@quality), -id=>$seqID );
}
=head2 get_seq_ids
Title : get_seq_ids
Usage : $contig->get_seq_ids( -start => $start,
-end => $end,
-type => "gapped A0QR67B08.b" );
Function : Get list of sequence IDs overlapping interval [$start, $end]
The default interval is [1,$contig->length]
Default coordinate system is "gapped contig"
Returns : An array
Argument : A hash with optional elements:
-start : consensus subsequence start
-end : consensus subsequence end
-type : the coordinate system type for $start and $end arguments
Coordinate system available are:
"gapped consensus" : consensus coordinates with gaps
"ungapped consensus" : consensus coordinates without gaps
"aligned $ReadID" : read $ReadID coordinates with gaps
"unaligned $ReadID" : read $ReadID coordinates without gaps
=cut
sub get_seq_ids {
my ($self, @args) = @_;
my ($type, $start, $end) = $self->_rearrange([qw(TYPE START END)], @args);
my @list;
if (defined($start) && defined($end)) {
if (defined($type) && ($type ne 'gapped consensus')) {
$start = $self->change_coord($type,'gapped consensus',$start);
$end = $self->change_coord($type,'gapped consensus',$end);
}
@list = $self->get_features_collection->features(
-type => '_aligned_coord', # primary tag
-start => $start,
-end => $end,
#-contain => 0,
#-strandmatch => 'ignore',
);
@list = map { $_->entire_seq->id } @list;
} else {
# Entire aligned sequences list
@list = map { $self->{'_order'}{$_} } sort { $a cmp $b } keys %{ $self->{'_order'} };
}
return @list;
}
=head2 get_seq_feat_by_tag
Title : get_seq_feat_by_tag
Usage : $seq = $contig->get_seq_feat_by_tag($seq,"_aligned_coord:$seqID")
Function : Get a sequence feature based on its primary_tag.
Returns : a Bio::SeqFeature object
Argument : a Bio::LocatableSeq and a string (feature primary tag)
=cut
sub get_seq_feat_by_tag {
my ($self,$seq,$tag) = @_;
if( !ref $seq || ! $seq->isa('Bio::LocatableSeq') ) {
$self->throw("Unable to process non locatable sequences [".ref($seq)."]");
}
my $seqID = $seq->id || $seq->display_id || $seq->primary_id;
return $self->{'_elem'}{$seqID}{'_feat'}{$tag};
}
=head2 get_seq_by_name
Title : get_seq_by_name
Usage : $seq = $contig->get_seq_by_name('Seq1')
Function : Gets a sequence based on its id.
Returns : a Bio::LocatableSeq object
undef if name is not found
Argument : string
=cut
sub get_seq_by_name {
my $self = shift;
my ($seqID) = @_;
unless (exists $self->{'_elem'}{$seqID}{'_seq'}) {
$self->throw("Could not find sequence $seqID in contig ".$self->id);
return;
}
return $self->{'_elem'}{$seqID}{'_seq'};
}
=head2 get_qual_by_name
Title : get_qual_by_name
Usage : $seq = $contig->get_qual_by_name('Seq1')
Function :
Gets Bio::Seq::QualI object for a sequence
through its id ( as given by $qual->id() ).
Returns : a Bio::Seq::QualI object.
undef if name is not found
Argument : string
=cut
sub get_qual_by_name {
my $self = shift;
my ($seqID) = @_;
unless (exists $self->{'_elem'}{$seqID}{'_qual'}) {
$self->warn("Could not find quality for $seqID in contig!");
return;
}
return $self->{'_elem'}{$seqID}{'_qual'};
}
=head1 Bio::Align::AlignI compatible methods
=head2 Modifier methods
These methods modify the MSE by adding, removing or shuffling complete
sequences.
=head2 add_seq
Title : add_seq
Usage : $contig->add_seq($newseq);
Function :
Adds a sequence to the contig. *Does*
*not* align it - just adds it to the
hashes.
Returns : nothing
Argument : a Bio::LocatableSeq object
See L<Bio::LocatableSeq> for more information.
=cut
sub add_seq {
my $self = shift;
my $seq = shift;
if( !ref $seq || ! $seq->isa('Bio::LocatableSeq') ) {
$self->throw("Unable to process non locatable sequences [".ref($seq)."]");
}
my $seqID = $seq->id() || $seq->display_id || $seq->primary_id;
$self->{'_elem'}{$seqID} = {} unless (exists $self->{'_elem'}{$seqID});
if (exists( $self->{'_elem'}{$seqID}{'_seq'} ) &&
($seq eq $self->{'_elem'}{$seqID}{'_seq'}) ) {
$self->warn("Adding sequence $seqID, which has already been added");
}
# Our locatable sequences are always considered to be complete sequences
$seq->start(1);
$seq->end($seq->_ungapped_len);
my $alphabet = $seq->alphabet;
$alphabet = lc($alphabet) if defined $alphabet;
$self->warn("Adding non-nucleotidic sequence ".$seqID)
if (!$alphabet || ($alphabet ne 'dna' && $alphabet ne 'rna'));
# build the symbol list for this sequence,
# will prune out the gap and missing/match chars
# when actually asked for the symbol list in the
# symbol_chars
if (defined $seq->seq) {
map { $self->{'_symbols'}->{$_} = 1; } split(//,$seq->seq);
} else {
$self->{'_symbols'} = {};
}
my $seq_no = ++$self->{'_nof_seqs'};
if (ref( $self->{'_elem'}{$seqID}{'_seq'} )) {
$self->warn("Replacing one sequence [$seqID]\n");
} else {
#print STDERR "Assigning $seqID to $order\n";
$self->{'_order'}->{$seq_no} = $seqID;
# $self->{'_start_end_lists'}->{$id} = []
# unless(exists $self->{'_start_end_lists'}->{$id});
# push @{$self->{'_start_end_lists'}->{$id}}, $seq;
}
$self->{'_elem'}{$seqID}{'_seq'} = $seq;
$self->{'_elem'}{$seqID}{'_feat'} = {};
$self->{'_elem'}{$seqID}{'_gaps'} = [];
my $dbref = $self->{'_elem'}{$seqID}{'_gaps'};
my $nofgaps = $self->_register_gaps($seq->seq,$dbref);
# Updating residue count
$self->{'_nof_residues'} += $seq->length - $nofgaps;
return 1;
}
=head2 remove_seq
Title : remove_seq
Usage : $contig->remove_seq($seq);
Function : Removes a single sequence from a contig
Returns : 1 on success, 0 otherwise
Argument : a Bio::LocatableSeq object
=cut
sub remove_seq {
my ($self,$seq) = @_;
if( !ref $seq || ! $seq->isa('Bio::LocatableSeq') ) {
$self->throw("Unable to process non locatable sequences [".ref($seq)."]");
}
my $seqID = $seq->id() || $seq->display_id || $seq->primary_id;
unless (exists $self->{'_elem'}{$seqID} ) {
$self->warn("No sequence named $seqID [$seq]");
return 0;
}
# Updating residue count
$self->{'_nof_residues'} -= $seq->length() +
&_nof_gaps( $self->{'_elem'}{$seqID}{'_gaps'}, $seq->length );
# Update number of sequences
$self->{'_nof_seqs'}--;
# Update order of sequences (order starts at 1)
my $max_order = $self->{'_nof_seqs'} + 1;
my $target_order = $max_order + 1;
for (my $order = 1 ; $order <= $max_order ; $order++) {
if ($self->{'_order'}->{$order} eq $seqID) {
# Found the wanted sequence order
$target_order = $order;
}
if ($order > $target_order) {
# Decrement this sequence order by one order
$self->{'_order'}->{$order-1} = $self->{'_order'}->{$order};
}
if ($order == $max_order) {
# Remove last order
delete $self->{'_order'}->{$order};
}
}
# Remove all references to features of this sequence
my @feats = ();
for my $tag (keys %{ $self->{'_elem'}{$seqID}{'_feat'} }) {
push(@feats, $self->{'_elem'}{$seqID}{'_feat'}{$tag});
}
$self->{'_sfc'}->remove_features(\@feats);
delete $self->{'_elem'}{$seqID};
return 1;
}
=head2 purge
Title : purge
Usage : $contig->purge(0.7);
Function:
Removes sequences above whatever %id.
This function will grind on large alignments. Beware!
(perhaps not ideally implemented)
Example :
Returns : An array of the removed sequences
Argument:
=cut
sub purge {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 sort_alphabetically
Title : sort_alphabetically
Usage : $contig->sort_alphabetically
Function :
Changes the order of the alignemnt to alphabetical on name
followed by numerical by number.
Returns :
Argument :
=cut
sub sort_alphabetically {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 Sequence selection methods
Methods returning one or more sequences objects.
=head2 each_seq
Title : each_seq
Usage : foreach $seq ( $contig->each_seq() )
Function : Gets an array of Seq objects from the alignment
Returns : an array
Argument :
=cut
sub each_seq {
my ($self) = @_;
my (@arr,$seqID);
foreach $seqID ( map { $self->{'_order'}{$_} } sort { $a <=> $b } keys %{$self->{'_order'}} ) {
push(@arr,$self->{'_elem'}{$seqID}{'_seq'});
}
return @arr;
}
=head2 each_alphabetically
Title : each_alphabetically
Usage : foreach $seq ( $contig->each_alphabetically() )
Function :
Returns an array of sequence object sorted alphabetically
by name and then by start point.
Does not change the order of the alignment
Returns :
Argument :
=cut
sub each_alphabetically {
my($self) = @_;
$self->throw_not_implemented();
}
=head2 each_seq_with_id
Title : each_seq_with_id
Usage : foreach $seq ( $contig->each_seq_with_id() )
Function :
Gets an array of Seq objects from the
alignment, the contents being those sequences
with the given name (there may be more than one)
Returns : an array
Argument : a seq name
=cut
sub each_seq_with_id {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 get_seq_by_pos
Title : get_seq_by_pos
Usage : $seq = $contig->get_seq_by_pos(3)
Function :
Gets a sequence based on its position in the alignment.
Numbering starts from 1. Sequence positions larger than
num_sequences() will thow an error.
Returns : a Bio::LocatableSeq object
Argument : positive integer for the sequence osition
=cut
sub get_seq_by_pos {
my $self = shift;
my ($pos) = @_;
$self->throw("Sequence position has to be a positive integer, not [$pos]")
unless $pos =~ /^\d+$/ and $pos > 0;
$self->throw("No sequence at position [$pos]")
unless $pos <= $self->num_sequences ;
my $seqID = $self->{'_order'}->{--$pos};
return $self->{'_elem'}{$seqID}{'_seq'};
}
=head2 Create new alignments
The result of these methods are horizontal or vertical subsets of the
current MSE.
=head2 select
Title : select
Usage : $contig2 = $contig->select(1, 3) # three first sequences
Function :
Creates a new alignment from a continuous subset of
sequences. Numbering starts from 1. Sequence positions
larger than num_sequences() will thow an error.
Returns : a Bio::Assembly::Contig object
Argument : positive integer for the first sequence
positive integer for the last sequence to include (optional)
=cut
sub select {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 select_noncont
Title : select_noncont
Usage : $contig2 = $contig->select_noncont(1, 3) # first and 3rd sequences
Function :
Creates a new alignment from a subset of
sequences. Numbering starts from 1. Sequence positions
larger than num_sequences() will throw an error.
Returns : a Bio::Assembly::Contig object
Args : array of integers for the sequences
=cut
sub select_noncont {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 slice
Title : slice
Usage : $contig2 = $contig->slice(20, 30)
Function :
Creates a slice from the alignment inclusive of start and
end columns. Sequences with no residues in the slice are
excluded from the new alignment and a warning is printed.
Slice beyond the length of the sequence does not do
padding.
Returns : a Bio::Assembly::Contig object
Argument : positive integer for start column
positive integer for end column
=cut
sub slice {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 Change sequences within the MSE
These methods affect characters in all sequences without changeing the
alignment.
=head2 map_chars
Title : map_chars
Usage : $contig->map_chars('\.','-')
Function :
Does a s/$arg1/$arg2/ on the sequences. Useful for gap
characters
Notice that the from (arg1) is interpretted as a regex,
so be careful about quoting meta characters (eg
$contig->map_chars('.','-') wont do what you want)
Returns :
Argument : 'from' rexexp
'to' string
=cut
sub map_chars {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 uppercase
Title : uppercase()
Usage : $contig->uppercase()
Function : Sets all the sequences to uppercase
Returns :
Argument :
=cut
sub uppercase {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 match_line
Title : match_line()
Usage : $contig->match_line()
Function : Generates a match line - much like consensus string
except that a line indicating the '*' for a match.
Argument : (optional) Match line characters ('*' by default)
(optional) Strong match char (':' by default)
(optional) Weak match char ('.' by default)
=cut
sub match_line {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 match
Title : match()
Usage : $contig->match()
Function :
Goes through all columns and changes residues that are
identical to residue in first sequence to match '.'
character. Sets match_char.
USE WITH CARE: Most MSE formats do not support match
characters in sequences, so this is mostly for output
only. NEXUS format (Bio::AlignIO::nexus) can handle
it.
Returns : 1
Argument : a match character, optional, defaults to '.'
=cut
sub match {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 unmatch
Title : unmatch()
Usage : $contig->unmatch()
Function :
Undoes the effect of method match. Unsets match_char.
Returns : 1
Argument : a match character, optional, defaults to '.'
=cut
sub unmatch {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 MSE attibutes
Methods for setting and reading the MSE attributes.
Note that the methods defining character semantics depend on the user
to set them sensibly. They are needed only by certain input/output
methods. Unset them by setting to an empty string ('').
=head2 id
Title : id
Usage : $contig->id("Ig")
Function : Gets/sets the id field of the alignment
Returns : An id string
Argument : An id string (optional)
=cut
sub id {
my ($self, $contig_name) = @_;
if (defined( $contig_name )) {
$self->{'_id'} = $contig_name;
}
return $self->{'_id'};
}
=head2 missing_char
Title : missing_char
Usage : $contig->missing_char("?")
Function : Gets/sets the missing_char attribute of the alignment
It is generally recommended to set it to 'n' or 'N'
for nucleotides and to 'X' for protein.
Returns : An missing_char string,
Argument : An missing_char string (optional)
=cut
sub missing_char {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 match_char
Title : match_char
Usage : $contig->match_char('.')
Function : Gets/sets the match_char attribute of the alignment
Returns : An match_char string,
Argument : An match_char string (optional)
=cut
sub match_char {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 gap_char
Title : gap_char
Usage : $contig->gap_char('-')
Function : Gets/sets the gap_char attribute of the alignment
Returns : An gap_char string, defaults to '-'
Argument : An gap_char string (optional)
=cut
sub gap_char {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 symbol_chars
Title : symbol_chars
Usage : my @symbolchars = $contig->symbol_chars;
Function: Returns all the seen symbols (other than gaps)
Returns : array of characters that are the seen symbols
Argument: boolean to include the gap/missing/match characters
=cut
sub symbol_chars{
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 Alignment descriptors
These read only methods describe the MSE in various ways.
=head2 consensus_string
Title : consensus_string
Usage : $str = $contig->consensus_string($threshold_percent)
Function : Makes a strict consensus
Returns :
Argument : Optional threshold ranging from 0 to 100.
The consensus residue has to appear at least threshold %
of the sequences at a given location, otherwise a '?'
character will be placed at that location.
(Default value = 0%)
=cut
sub consensus_string {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 consensus_iupac
Title : consensus_iupac
Usage : $str = $contig->consensus_iupac()
Function :
Makes a consensus using IUPAC ambiguity codes from DNA
and RNA. The output is in upper case except when gaps in
a column force output to be in lower case.
Note that if your alignment sequences contain a lot of
IUPAC ambiquity codes you often have to manually set
alphabet. Bio::PrimarySeq::_guess_type thinks they
indicate a protein sequence.
Returns : consensus string
Argument : none
Throws : on protein sequences
=cut
sub consensus_iupac {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 is_flush
Title : is_flush
Usage : if( $contig->is_flush() )
:
:
Function : Tells you whether the alignment
: is flush, ie all of the same length
:
:
Returns : 1 or 0
Argument :
=cut
sub is_flush {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 length
Title : length()
Usage : $len = $contig->length()
Function : Returns the maximum length of the alignment.
To be sure the alignment is a block, use is_flush
Returns :
Argument :
=cut
sub length {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 maxname_length
Title : maxname_length
Usage : $contig->maxname_length()
Function :
Gets the maximum length of the displayname in the
alignment. Used in writing out various MSE formats.
Returns : integer
Argument :
=cut
sub maxname_length {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 num_residues
Title : num_residues
Usage : $no = $contig->num_residues
Function : number of residues in total in the alignment
Returns : integer
Argument :
Note : replaces no_residues
=cut
sub num_residues {
my ($self) = @_;
return $self->{'_nof_residues'};
}
=head2 num_sequences
Title : num_sequences
Usage : $depth = $contig->num_sequences
Function : number of sequence in the sequence alignment
Returns : integer
Argument : None
Note : replaces no_sequences
=cut
sub num_sequences {
my ($self) = @_;
return scalar( keys %{ $self->{'_elem'} } );
}
=head2 percentage_identity
Title : percentage_identity
Usage : $id = $contig->percentage_identity
Function: The function calculates the percentage identity of the alignment
Returns : The percentage identity of the alignment (as defined by the
implementation)
Argument: None
=cut
sub percentage_identity{
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 overall_percentage_identity
Title : percentage_identity
Usage : $id = $contig->percentage_identity
Function: The function calculates the percentage identity of
the conserved columns
Returns : The percentage identity of the conserved columns
Args : None
=cut
sub overall_percentage_identity{
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 average_percentage_identity
Title : average_percentage_identity
Usage : $id = $contig->average_percentage_identity
Function: The function uses a fast method to calculate the average
percentage identity of the alignment
Returns : The average percentage identity of the alignment
Args : None
=cut
sub average_percentage_identity {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 Alignment positions
Methods to map a sequence position into an alignment column and back.
column_from_residue_number() does the former. The latter is really a
property of the sequence object and can done using
L<Bio::LocatableSeq::location_from_column>:
# select somehow a sequence from the alignment, e.g.
my $seq = $contig->get_seq_by_pos(1);
#$loc is undef or Bio::LocationI object
my $loc = $seq->location_from_column(5);
=head2 column_from_residue_number
Title : column_from_residue_number
Usage : $col = $contig->column_from_residue_number( $seqname, $resnumber)
Function:
This function gives the position in the alignment
(i.e. column number) of the given residue number in the
sequence with the given name. For example, for the
alignment
Seq1/91-97 AC..DEF.GH
Seq2/24-30 ACGG.RTY..
Seq3/43-51 AC.DDEFGHI
column_from_residue_number( "Seq1", 94 ) returns 5.
column_from_residue_number( "Seq2", 25 ) returns 2.
column_from_residue_number( "Seq3", 50 ) returns 9.
An exception is thrown if the residue number would lie
outside the length of the aligment
(e.g. column_from_residue_number( "Seq2", 22 )
Note: If the the parent sequence is represented by more than
one alignment sequence and the residue number is present in
them, this method finds only the first one.
Returns : A column number for the position in the alignment of the
given residue in the given sequence (1 = first column)
Args : A sequence id/name (not a name/start-end)
A residue number in the whole sequence (not just that
segment of it in the alignment)
=cut
sub column_from_residue_number {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 Sequence names
Methods to manipulate the display name. The default name based on the
sequence id and subsequence positions can be overridden in various
ways.
=head2 displayname
Title : displayname
Usage : $contig->displayname("Ig", "IgA")
Function : Gets/sets the display name of a sequence in the alignment
:
Returns : A display name string
Argument : name of the sequence
displayname of the sequence (optional)
=cut
sub displayname { # Do nothing
}
=head2 set_displayname_count
Title : set_displayname_count
Usage : $contig->set_displayname_count
Function :
Sets the names to be name_# where # is the number of
times this name has been used.
Returns : None
Argument : None
=cut
sub set_displayname_count {
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 set_displayname_flat
Title : set_displayname_flat
Usage : $contig->set_displayname_flat()
Function : Makes all the sequences be displayed as just their name,
not name/start-end
Returns : 1
Argument : None
=cut
sub set_displayname_flat { # Do nothing!
}
=head2 set_displayname_normal
Title : set_displayname_normal
Usage : $contig->set_displayname_normal()
Function : Makes all the sequences be displayed as name/start-end
Returns : None
Argument : None
=cut
sub set_displayname_normal { # Do nothing!
}
=head1 Internal Methods
=head2 _binary_search
Title : _binary_search
Usage : _binary_search($list,$query)
Function :
Find a number in a sorted list of numbers. Return values
may be on or two integers. One positive integer or zero
(>=0) is the index of the element that stores the queried
value. Two positive integers (or zero and another
number) are the indexes of elements among which the
queried value should be placed. Negative single values
mean:
-1: $query is smaller than smallest element in list
-2: $query is greater than greatest element in list
Returns : array of integers
Argument :
$list : array reference
$query : integer
=cut
sub _binary_search {
my $list = shift;
my $query = shift;
#
# If there is only one element in list
if (!$#{$list} && ($query == $list->[0])) { return (0) }
# If there are others...
my $start = 0;
my $end = $#{$list};
(&_compare($query,$list->[$start]) == 0) && do { return ($start) };
(&_compare($query,$list->[$end]) == 0) && do { return ($end) };
(&_compare($query,$list->[$start]) < 0) && do { return (-1) };
(&_compare($query,$list->[$end]) > 0) && do { return (-2) };
my $middle = 0;
while ($end - $start > 1) {
$middle = int(($end+$middle)/2);
(&_compare($query,$list->[$middle]) == 0) && return ($middle);
(&_compare($query,$list->[$middle]) < 0) && do { $end = $middle ; $middle = 0; next };
$start = $middle; # If &_compare() > 0, move region beggining
}
return ($start,$end);
}
=head2 _compare
Title : _compare
Usage : _compare($arg1,$arg2)
Function: Perform numeric or string comparisons
Returns : integer (0, 1 or -1)
Args : values to be compared
=cut
sub _compare {
my $arg1 = shift;
my $arg2 = shift;
#
if (($arg1 =~ /^\d+$/) && ($arg2 =~ /^\d+$/)) { return $arg1 <=> $arg2 }
else { return $arg1 cmp $arg2 }
}
=head2 _nof_gaps
Title : _nof_gaps
Usage : _nof_gaps($array_ref, $query)
Function: number of gaps found before position $query
Returns : integer
Args :
$array_ref : gap registry reference
$query : [integer] a position in a sequence
=cut
#' emacs...
sub _nof_gaps {
my $list = shift;
my $query = shift;
# If there are no gaps in this contig
return 0 unless (defined($list) && scalar(@{$list}));
# Locate query index in gap list (if any)
my @index = &_binary_search($list,$query);
# If after all alignments, correct using total number of align
if ($index[0] == -2) { $query = scalar(@{$list}) }
# If before any alignment, return 0
elsif ($index[0] == -1) { $query = 0 }
elsif ($index[0] >= 0) {
# If query is between alignments, translate coordinates
if ($#index > 0) { $query = $index[0] + 1 }
# If query sits upon an alignment, do another correction
elsif ($#index == 0) { $query = $index[0] }
}
#
return $query;
}
=head2 _padded_unpadded
Title : _padded_unpadded
Usage : _padded_unpadded($array_ref, $query)
Function:
Returns a coordinate corresponding to
position $query after gaps were
removed from a sequence.
Returns : integer
Args :
$array_ref : reference to this gap registry
$query : [integer] coordionate to change
=cut
sub _padded_unpadded {
my $list = shift;
my $query = shift;
my $align = &_nof_gaps($list,$query);
$query-- if (defined($list->[$align]) && ($list->[$align] == $query));
$query = $query - $align;
#
return $query;
}
=head2 _unpadded_padded
Title : _unpadded_padded
Usage : _unpadded_padded($array_ref, $query)
Function:
Returns the value corresponding to
ungapped position $query when gaps are
counted as valid sites in a sequence
Returns :
Args : $array_ref = a reference to this sequence's gap registry
$query = [integer] location to change
=cut
#'
sub _unpadded_padded {
my $list = shift;
my $query = shift;
my $align = &_nof_gaps($list,$query);
$query = $query + $align;
my $new_align = &_nof_gaps($list,$query);
while ($new_align - $align > 0) {
$query = $query + $new_align - $align;
$align = $new_align;
$new_align = &_nof_gaps($list,$query);
}
# If current position is also a align, look for the first upstream base
while (defined($list->[$align]) && ($list->[$align] == $query)) {
$query++; $align++;
}
#
return $query;
}
=head2 _register_gaps
Title : _register_gaps
Usage : $self->_register_gaps($seq, $array_ref)
Function: stores gap locations for a sequence
Returns : number of gaps found
Args :
$seq : sequence string
$array_ref : a reference to an array,
where gap locations will
be stored
=cut
sub _register_gaps {
my $self = shift;
my $sequence = shift;
my $dbref = shift;
$self->throw("Not an aligned sequence string to register gaps")
if (ref($sequence));
$self->throw("Not an array reference for gap registry")
unless (ref($dbref) eq 'ARRAY');
# Registering alignments
@{$dbref} = (); # Cleaning registry
if (defined $sequence) {
my $i = -1;
while(1) {
$i = index($sequence,"-",$i+1);
last if ($i == -1);
push(@{$dbref},$i+1);
}
} else {
# $self->warn("Found undefined sequence while registering gaps");
return 0;
}
return scalar(@{$dbref});
}
=head1 Deprecated methods
=cut
=head2 no_residues
Title : no_residues
Usage : $no = $ali->no_residues
Function : number of residues in total in the alignment
Returns : integer
Argument :
Note : deprecated in favor of num_residues()
=cut
sub no_residues {
my $self = shift;
$self->deprecated(-warn_version => 1.0069,
-throw_version => 1.0075);
$self->num_residues(@_);
}
=head2 no_sequences
Title : no_sequences
Usage : $depth = $ali->no_sequences
Function : number of sequence in the sequence alignment
Returns : integer
Argument :
Note : deprecated in favor of num_sequences()
=cut
sub no_sequences {
my $self = shift;
$self->deprecated(-warn_version => 1.0069,
-throw_version => 1.0075);
$self->num_sequences(@_);
}
1;