LOCUS PNECG 2355 bp DNA circular BCT 21-APR-1996
DEFINITION Plasmid pNE131 (from S.epidermidis), complete genome.
ACCESSION M12730
VERSION M12730.1 GI:150829
KEYWORDS MLS antibiotics resistance; complete genome;
macrolide-lincosamide-streptogramin B resistance; plasmid;
replication and maintenance protein.
SOURCE Plasmid pNE131
ORGANISM Plasmid pNE131
other sequences; plasmids; Plasmid.
REFERENCE 1 (bases 696 to 1586)
AUTHORS Lampson,B.C. and Parisi,J.T.
TITLE Naturally occurring Staphylococcus epidermidis plasmid expressing
constitutive macrolide-lincosamide-streptogramin B resistance
contains a deleted attenuator
JOURNAL J. Bacteriol. 166 (2), 479-483 (1986)
PUBMED 3084450
REFERENCE 2 (bases 1 to 2355)
AUTHORS Lampson,B.C. and Parisi,J.T.
TITLE Nucleotide sequence of the constitutive
macrolide-lincosamide-streptogramin B resistance plasmid pNE131
from Staphylococcus epidermidis and homologies with Staphylococcus
aureus plasmids pE194 and pSN2
JOURNAL J. Bacteriol. 167 (3), 888-892 (1986)
PUBMED 3091582
COMMENT Original source text: Plasmid pNE131 (clone: pNE131.) DNA. Draft
entry and clean copy sequence for [2] kindly provided by
J.T.Parisi, 04-AUG-1986. Ribosome binding sites are located at
positions 754-759, 769-773, and 1944-1948; a -35 region at
1889-1899, and -10 regions at 733-738, and 1927-1932.
FEATURES Location/Qualifiers
source 1..2355
/db_xref="taxon:2608"
/mol_type="genomic DNA"
/clone="pNE131."
/specific_host="Staphylococcus epidermidis"
/plasmid="Plasmid pNE131"
/organism="Plasmid pNE131"
CDS join(1959..2355,1..92)
/db_xref="GI:150830"
/codon_start=1
/protein_id="AAA98295.1"
/translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIV
QLISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEE
GNIIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALYLIIILSRTS
IT"
/product="replication and maintenance protein"
/transl_table=11
gene 706..1550
/gene="ermM"
-35_signal 706..715
/gene="ermM"
-10_signal 733..738
/gene="ermM"
mRNA 746..1550
/gene="ermM"
CDS 780..1514
/protein_id="AAA98296.1"
/gene="ermM"
/transl_table=11
/db_xref="GI:150831"
/codon_start=1
/product="macrolide-lincosamide-streptogramin
B-resistance protein"
/translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
TLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIFGNI
PYNISTDIIRKIVFDSIADEIYLIVEYGFAKRLLNTKRSFALFLMAEVDISILSMVPR
EYFHPKPKVNSSLIRLNRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
AGIDDLNNISFEQFLSLFNSYKLFNK"
terminator 1513..1546
/gene="ermM"
/note="putative"
BASE COUNT 911 a 359 c 371 g 714 t
ORIGIN 1 bp upstream of TaqI site.
1 tcgaatttgg gaactttgag caagaggcaa atgaaaaaca agaaaatgca ctttatctga
61 ttattattct ttcaaggact agtataacat aaatcgtcta caaatagaca aaaaacctgc
121 acgcttaatg tagatcaaaa gcttaacgca aatgaaatag attgacctcc caataacacc
181 acgttagtta ttgggagtca atctatgaaa tgcgattaag ctttttctaa ttcacataag
241 cgtgcaggtt taaagtacat aaaaaatata atgaaaaaaa gcatcattat actaacgtta
301 taccaacatt atactaattg cttattccaa tttcctattg gttggaacca acaggcgtta
361 gtgtgttgtt gagttggtac tttcatggga ttaatcccat gaaaccccca accaactcgc
421 caaagctttg gctaacacac acgccattcc aaccaatagt tttctcggca ttaaagccat
481 gctctgacgc ttaaatgcac taatgcctta aaaaaacatt aaagtctaac acactagact
541 tattttcatt cgtaattaag tcgttaaacc gtgtgctcta cgaccaaaag tataaaacct
601 ttaagaactt tcttttttct tgtaaaaaaa gaaactagat aaatctctca tatcttttat
661 tcaataatcg catcagattg cagtataaat ttaacgatca ctcatcatgt tcatatttat
721 cagagctcgt gctataatta tactaatttt ataaggagga aaaaataaag agggttataa
781 tgaacgagaa aaatataaaa cacagtcaaa actttattac ttcaaaacat aatatagata
841 aaataatgac aaatataaga ttaaatgaac atgataatat ctttgaaatc ggctcaggaa
901 aagggcattt tacccttgaa ttagtacaga ggtgtaattt cgtaactgcc attgaaatag
961 accataaatt atgcaaaact acagaaaata aacttgttga tcacgataat ttccaagttt
1021 taaacaagga tatattgcag tttaaatttc ctaaaaacca atcctataaa atatttggta
1081 atatacctta taacataagt acggatataa tacgcaaaat tgtttttgat agtatagctg
1141 atgagattta tttaatcgtg gaatacgggt ttgctaaaag attattaaat acaaaacgct
1201 cattcgcatt atttttaatg gcagaagttg atatttctat attaagtatg gttccaagag
1261 aatattttca tcctaaacct aaagtgaata gctcacttat cagattaaat agaaaaaaat
1321 caagaatatc acacaaagat aaacagaagt ataattattt cgttatgaaa tgggttaaca
1381 aagaatacaa gaaaatattt acaaaaaatc aatttaacaa ttccttaaaa catgcaggaa
1441 ttgacgattt aaacaatatt agctttgaac aattcttatc tcttttcaat agctataaat
1501 tatttaataa gtaagttaag ggatgcataa actgcatcct taacttgttt ttcgtgtacc
1561 tattttttgt gaatcgatta tgtcttttgc gcattcactt cttttctata taaatatgag
1621 cgacagataa gcgtcggaaa agcagcaaaa agtttccttt ttgctgttgg agcatggggg
1681 ttcagggggt gcagtatctg acgtcaatgc cgagcgaaag cgagccgaag ggtagcattt
1741 acgttagata accccctgat atgctccgac gctttatata gaaaagaaga ttcaactagg
1801 taaaatctta atataggttg agatgataag gtttataagg aatttgtttg ttctaatttt
1861 tcactcattt tgttctaatt tcttttaaca aatgttcttt tttttttaga acagttatga
1921 tatagttaga atagtttaaa ataaggagtg agaaaaagat gaaagaaaga tatggaacag
1981 tctataaagg ctctcagagg ctcatagacg aagaaagtgg agaagtcata gaggtagaca
2041 agttataccg taaacaaacg tctggtaact tcgtaaaggc atatatagtg caattaataa
2101 gtatgttaga tatgattggc ggaaaaaaac ttaaaatcgt taactatatc ctagataatg
2161 tccacttaag taacaataca atgatagcta caacaagaga aatagcaaaa gctacaggaa
2221 caagtctaca aacagtaata acaacactta aaatcttaga agaaggaaat attataaaaa
2281 gaaaaactgg agtattaatg ttaaaccctg aactactaat gagaggcgac gaccaaaaac
2341 aaaaatacct cttac
//