BLASTX 2.0MP-WashU [12-Feb-2001] [linux-i686 01:36:08 31-Jan-2001]

Copyright (C) 1996-2000 Washington University, Saint Louis, Missouri USA.
All Rights Reserved.

Reference:  Gish, W. (1996-2000) http://blast.wustl.edu
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice:  statistical significance is estimated under the assumption that the
equivalent of one entire reading frame in the query sequence codes for protein
and that significant alignments will involve only coding reading frames.

Query=  gi|142864|gb|M10040.1|BACDNAE B.subtilis dnaE gene encoding DNA
    primase, complete cds
        (2001 letters)

  Translating both strands of query sequence in all 6 reading frames

Database:  ecoli.aa
           4289 sequences; 1,358,990 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done

                                                                     Smallest
                                                                       Sum
                                                              High  Probability
Sequences producing High-scoring Segment Pairs:              Score  P(N)      N

gi|1789447|gb|AAC76102.1| (AE000388) DNA biosynthesis; DN...   671  1.1e-74   1



>gi|1789447|gb|AAC76102.1| (AE000388) DNA biosynthesis; DNA primase
            [Escherichia coli]
        Length = 581

  Plus Strand HSPs:

 Score = 671 (265.8 bits), Expect = 1.1e-74, P = 1.1e-74
 Identities = 151/421 (35%), Positives = 223/421 (52%), Frame = +3

Query:    21 MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFH 200
             M  RIP   ++ +    DIV++I   V+LKKQG+N+   CPFH E TPSF+V+ +KQ +H
Sbjct:     1 MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHNEKTPSFTVNGEKQFYH 60

Query:   201 CFGCGAGGNVFSFLRQMEGYSFAESVSHLADKYQIDFPDDITVHSGARP---ESSGEQKM 371
             CFGCGA GN   FL   +   F E+V  LA  + ++ P +    +G+ P   E    Q +
Sbjct:    61 CFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFE----AGSGPSQIERHQRQTL 116

Query:   372 AEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQIGYALDSWDFITKFLVKR 551
              +  + L  FY   L        A  YL  RG + E+I  F IG+A   WD + K     
Sbjct:   117 YQLMDGLNTFYQQSL-QQPVATSARQYLEKRGLSHEVIARFAIGFAPPGWDNVLKRFGGN 175

Query:   552 GFSEAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSP 731
               +   +  AG+L+  + G  Y DRFR RVMFPI D  G V+ F GR LG+  PKY+NSP
Sbjct:   176 PENRQSLIDAGMLVTNDQGRSY-DRFRERVMFPIRDKRGRVIGFGGRVLGNDTPKYLNSP 234

Query:   732 ETPLFHKSKLLYNFYKARLHIRKQERAVLFEGFADVYTAVSSDVKESIATMGTSLTDDHV 911
             ET +FHK + LY  Y+A+    +  R ++ EG+ DV       +  ++A++GTS T DH+
Sbjct:   235 ETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQYGINYAVASLGTSTTADHI 294

Query:   912 KILRRNVEEIILCYDSDKAGYEATLKASELL---QKKGCKVRVAMIPDGLDPDDYIKKFG 1082
             ++L R    +I CYD D+AG +A  +A E        G ++R   +PDG DPD  ++K G
Sbjct:   295 QLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQLRFMFLPDGEDPDTLVRKEG 354

Query:  1083 GEKFKNDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEISTLSGSLEQEVYVK 1262
              E F+  + + ++ + AF         +LS    R       L  IS + G   + +Y++
Sbjct:   355 KEAFEARM-EQAMPLSAFLFNSLMPQVDLSTPDGRARLSTLALPLISQVPGETLR-IYLR 412

Query:  1263 Q 1265
             Q
Sbjct:   413 Q 413


Parameters:
  novalidctxok
  nonnegok
  gapall
  Q=12
  R=1
  cpus=1
  filter=seg
  matrix=blosum62
  W=3
  S2=41
  gapS2=68
  X=16
  gapX=38
  hitdist=40
  gi
  gapL=0.27
  gapK=0.047
  gapH=0.23

  ctxfactor=5.99
  E=10

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   +3      0   blosum62        0.318   0.135   0.401    0.324   0.139   0.405  
               Q=12,R=1        0.270   0.0470  0.230     n/a     n/a     n/a
   +2      0   blosum62        0.318   0.135   0.401    0.365   0.163   0.618  
               Q=12,R=1        0.270   0.0470  0.230     n/a     n/a     n/a
   +1      0   blosum62        0.318   0.135   0.401    0.356   0.155   0.528  
               Q=12,R=1        0.270   0.0470  0.230     n/a     n/a     n/a
   -1      0   blosum62        0.318   0.135   0.401    0.350   0.155   0.543  
               Q=12,R=1        0.270   0.0470  0.230     n/a     n/a     n/a
   -2      0   blosum62        0.318   0.135   0.401    0.350   0.155   0.505  
               Q=12,R=1        0.270   0.0470  0.230     n/a     n/a     n/a
   -3      0   blosum62        0.318   0.135   0.401    0.358   0.157   0.543  
               Q=12,R=1        0.270   0.0470  0.230     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      666       666       10.  59 3  12 16  0.021   41
                                                    38  0.0     59
   +2      0      666       666       10.  59 3  12 16  0.021   41
                                                    38  0.0     59
   +1      0      667       667       10.  59 3  12 16  0.021   41
                                                    38  0.0     59
   -1      0      667       667       10.  59 3  12 16  0.021   41
                                                    38  0.0     59
   -2      0      666       666       10.  59 3  12 16  0.021   41
                                                    38  0.0     59
   -3      0      666       666       10.  59 3  12 16  0.021   41
                                                    38  0.0     59


Statistics:

  Database:  /home/jes12/db/ecoli.aa
   Title:  ecoli.aa
   Posted:  2:52:35 PM EST Nov 18, 2001
   Created:  9:46:47 AM EST Nov 18, 2001
   Format:  XDF-1
   # of letters in database:  1,358,990
   # of sequences in database:  4289
   # of database sequences satisfying E:  1
  No. of states in DFA:  600 (64 KB)
  Total size of DFA:  655 KB (1283 KB)
  Time to generate neighborhood:  0.04u 0.01s 0.05t  Elapsed: 00:00:00
  No. of threads or processors used:  1
  Search cpu time:  0.44u 0.01s 0.45t  Elapsed: 00:00:01
  Total cpu time:  0.48u 0.02s 0.50t  Elapsed: 00:00:01
  Start:  Sat Apr 20 14:39:05 2002   End:  Sat Apr 20 14:39:06 2002