# -*-Perl-*- Test Harness script for Bioperl
# $Id$
use strict;
BEGIN {
use lib '.';
use Bio::Root::Test;
test_begin(-tests => 362);
use_ok 'Bio::Seq';
use_ok 'Bio::SeqIO';
use_ok 'Bio::SeqFeature::Generic';
}
my ($feat, $str, $feat2, $pair, @sft);
my $DEBUG = test_debug();
ok $feat = Bio::SeqFeature::Generic->new(
-start => 40,
-end => 80,
-strand => 1,
);
is $feat->primary_tag, '';
is $feat->source_tag, '';
is $feat->display_name, '';
ok $feat = Bio::SeqFeature::Generic->new(
-start => 40,
-end => 80,
-strand => 1,
-primary => 'exon',
-source => 'internal',
-display_name => 'my exon feature',
-tag => {
silly => 20,
new => 1
}
);
is $feat->start, 40, 'Start of feature location';
is $feat->end, 80, 'End of feature location';
is $feat->primary_tag, 'exon', 'Primary tag';
is $feat->source_tag, 'internal', 'Source tag';
is $feat->display_name, 'my exon feature', 'Display name';
is $feat->phase, undef, 'Undef phase by default';
is $feat->phase(1), 1, 'Phase accessor returns';
is $feat->phase, 1, 'Phase is persistent';
ok $feat->gff_string();
ok $feat2 = Bio::SeqFeature::Generic->new(
-start => 400,
-end => 440,
-strand => 1,
-primary => 'other',
-source => 'program_a',
-phase => 1,
-tag => {
silly => 20,
new => 1
}
);
is $feat2->phase, 1, 'Set phase from constructor';
# Test attaching a SeqFeature::Generic to a Bio::Seq or SeqFeature::Generic
{
# Make the parent sequence object
my $seq = Bio::Seq->new(
-seq => 'aaaaggggtttt',
-display_id => 'test',
-alphabet => 'dna',
);
# Make a SeqFeature
ok my $sf1 = Bio::SeqFeature::Generic->new(
-start => 4,
-end => 9,
-strand => 1,
);
# Add the SeqFeature to the parent
ok $seq->add_SeqFeature($sf1);
# Test that it gives the correct sequence
is $sf1->start, 4, 'Start of first seqfeature';
is $sf1->end, 9, 'End of first seqfeature';
is $sf1->strand, 1, 'Strand of first seqfeature';
ok my $sf_seq1 = $sf1->seq;
is $sf_seq1->seq, 'aggggt', 'Sequence of first seqfeature';
# Make a second seqfeature on the opposite strand
ok my $sf2 = Bio::SeqFeature::Generic->new(
-start => 4,
-end => 9,
-strand => -1,
);
# Now add the PrimarySeq to the seqfeature before adding it to the parent
ok $sf2->attach_seq($seq->primary_seq);
ok $seq->add_SeqFeature($sf2);
# Test again that we have the correct sequence
is $sf2->start, 4, 'Start of second seqfeature';
is $sf2->end, 9, 'End of second seqfeature';
is $sf2->strand, -1, 'Strand of second seqfeature';
ok my $sf_seq2 = $sf2->seq;
is $sf_seq2->seq, 'acccct', 'Sequence of second seqfeature';
}
# Some tests for bug #947
ok my $sfeat = Bio::SeqFeature::Generic->new(-primary => 'test');
ok $sfeat->add_sub_SeqFeature(
Bio::SeqFeature::Generic->new(
-start => 2,
-end => 4,
-primary => 'sub1'
),
'EXPAND'
);
ok $sfeat->add_sub_SeqFeature(
Bio::SeqFeature::Generic->new(
-start => 6,
-end => 8,
-primary => 'sub2'
),
'EXPAND'
);
is $sfeat->start, 2, 'sfeat start for EXPAND-ED feature (bug #947)';
is $sfeat->end, 8, 'sfeat end for EXPAND-ED feature (bug #947)';
# Some tests to see if we can set a feature to start at 0
ok $sfeat = Bio::SeqFeature::Generic->new(-start => 0, -end => 0 );
ok defined $sfeat->start;
is $sfeat->start, 0, 'Can create feature starting and ending at 0';
ok defined $sfeat->end;
is $sfeat->end, 0, 'Can create feature starting and ending at 0';
# Test for bug when Locations are not created explicitly
ok my $feat1 = Bio::SeqFeature::Generic->new(
-start => 1,
-end => 15,
-strand=> 1
);
ok $feat2 = Bio::SeqFeature::Generic->new(
-start => 10,
-end => 25,
-strand=> 1
);
ok my $overlap = $feat1->location->union($feat2->location);
is $overlap->start, 1;
is $overlap->end, 25;
ok my $intersect = $feat1->location->intersection($feat2->location);
is $intersect->start, 10;
is $intersect->end, 15;
# Now let's test spliced_seq
ok my $seqio = Bio::SeqIO->new(
-file => test_input_file('AY095303S1.gbk'),
-format => 'genbank'
);
isa_ok $seqio, 'Bio::SeqIO';
ok my $geneseq = $seqio->next_seq;
isa_ok $geneseq, 'Bio::Seq';
ok my ($CDS) = grep { $_->primary_tag eq 'CDS' } $geneseq->get_SeqFeatures;
my $db;
SKIP: {
test_skip(-tests => 5,
-requires_modules => [qw(IO::String
LWP::UserAgent
HTTP::Request::Common)],
-requires_networking => 1);
use_ok 'Bio::DB::GenBank';
$db = Bio::DB::GenBank->new(-verbose=> $DEBUG);
$CDS->verbose(-1);
my $cdsseq = $CDS->spliced_seq(-db => $db,-nosort => 1);
is $cdsseq->subseq(1,76),
'ATGCAGCCATACGCTTCCGTGAGCGGGCGATGTCTATCTAGACCAGATGCATTGCATGTGATACCGTTTGGGCGAC';
is $cdsseq->translate->subseq(1,100),
'MQPYASVSGRCLSRPDALHVIPFGRPLQAIAGRRFVRCFAKGGQPGDKKKLNVTDKLRLGNTPPTLDVLK'.
'APRPTDAPSAIDDAPSTSGLGLGGGVASPR';
# Test what happens without
$cdsseq = $CDS->spliced_seq(-db => $db,-nosort => 1);
is $cdsseq->subseq(1,76),
'ATGCAGCCATACGCTTCCGTGAGCGGGCGATGTCTATCTAGACCAGATGCATTGCATGTGATACCGTTTGGGCGAC';
is $cdsseq->translate->subseq(1,100),
'MQPYASVSGRCLSRPDALHVIPFGRPLQAIAGRRFVRCFAKGGQPGDKKKLNVTDKLRLGNTPPTLDVLK'.
'APRPTDAPSAIDDAPSTSGLGLGGGVASPR';
}
ok $seqio = Bio::SeqIO->new(
-file => test_input_file('AF032047.gbk'),
-format => 'genbank'
);
isa_ok $seqio, 'Bio::SeqIO';
ok $geneseq = $seqio->next_seq;
isa_ok $geneseq, 'Bio::Seq';
ok( ($CDS) = grep { $_->primary_tag eq 'CDS' } $geneseq->get_SeqFeatures );
SKIP: {
test_skip(-tests => 2,
-requires_modules => [qw(IO::String
LWP::UserAgent
HTTP::Request::Common)],
-requires_networking => 1);
my $cdsseq = $CDS->spliced_seq( -db => $db, -nosort => 1);
is $cdsseq->subseq(1,70),
'ATGGCTCGCTTCGTGGTGGTAGCCCTGCTCGCGCTACTCTCTCTGTCTGGCCTGGAGGCTATCCAGCATG';
is $cdsseq->translate->seq,
'MARFVVVALLALLSLSGLEAIQHAPKIQVYSRHPAENGKPNFLNCYVSGFHPSDIEVDLLKNGKKIEKVE'.
'HSDLSFSKDWSFYLLYYTEFTPNEKDEYACRVSHVTFPTPKTVKWDRTM*';
}
# Trans-spliced
ok $seqio = Bio::SeqIO->new(
-format => 'genbank',
-file => test_input_file('NC_001284.gbk')
);
isa_ok $seqio, 'Bio::SeqIO';
ok my $genome = $seqio->next_seq;
for my $cds (grep { $_->primary_tag eq 'CDS' } $genome->get_SeqFeatures) {
ok my $spliced = $cds->spliced_seq(-nosort => 1)->translate->seq;
chop $spliced; # remove stop codon
is $spliced, ($cds->get_tag_values('translation'))[0], 'spliced_seq translation matches expected';
}
# Spliced_seq phase
ok my $seq = Bio::SeqIO->new(
-format => 'fasta',
-file => test_input_file('sbay_c127.fas')
)->next_seq;
ok my $sf = Bio::SeqFeature::Generic->new(
-verbose => -1,
-start => 263,
-end => 721,
-strand => 1,
-primary => 'splicedgene'
);
ok $sf->attach_seq($seq);
my %phase_check = (
'TTCAATGACT' => 'FNDFYSMGKS',
'TCAATGACTT' => 'SMTSIPWVNQ',
'GTTCAATGAC' => 'VQ*LLFHG*I',
);
for my $phase (-1..3) {
ok my $sfseq = $sf->spliced_seq(-phase => $phase);
ok exists $phase_check{$sfseq->subseq(1,10)};
is $sfseq->translate->subseq(1,10), $phase_check{$sfseq->subseq(1,10)}, 'phase check';
}
# Tags
ok $sf->add_tag_value('note','n1');
ok $sf->add_tag_value('note','n2');
ok $sf->add_tag_value('comment','c1');
is_deeply [sort $sf->get_all_tags()], [sort qw(note comment)] , 'Tags found';
is_deeply [sort $sf->get_tagset_values('note')], [sort qw(n1 n2)] , 'get_tagset_values tag values found';
is_deeply [sort $sf->get_tagset_values(qw(note comment))], [sort qw(c1 n1 n2)] , 'get_tagset_values tag values for multiple tags found';
lives_ok {
is_deeply [sort $sf->get_tag_values('note')], [sort qw(n1 n2)] , 'get_tag_values tag values found';
} 'get_tag_values lives with tag';
lives_ok {
is_deeply [$sf->get_tagset_values('notag') ], [], 'get_tagset_values no tag values found';
} 'get_tagset_values lives with no tag';
throws_ok { $sf->get_tag_values('notag') } qr/tag value that does not exist/, 'get_tag_values throws with no tag';
# Circular sequence SeqFeature tests
$seq = Bio::SeqIO->new(
-format => 'genbank',
-file => test_input_file('PX1CG.gb')
)->next_seq;
ok $seq->is_circular, 'Phi-X174 genome is circular';
# Retrieving the spliced sequence from any split location requires spliced_seq()
my %sf_data = (
# start
'A' => [3981, 136, 1, 1542, 'join(3981..5386,1..136)', 'ATGGTTCGTT'],
'A*' => [4497, 136, 1, 1026, 'join(4497..5386,1..136)', 'ATGAAATCGC'],
'B' => [5075, 136, 1, 363, 'join(5075..5386,1..51)', 'ATGGAACAAC'],
);
ok my @split_sfs = grep {
$_->location->isa('Bio::Location::SplitLocationI')
} $seq->get_SeqFeatures();
is @split_sfs, 3, 'Only 3 split locations';
for my $sf (@split_sfs) {
isa_ok $sf->location, 'Bio::Location::SplitLocationI';
ok my ($tag) = $sf->get_tag_values('product');
my ($start, $end, $strand, $length, $ftstring, $first_ten) = @{$sf_data{$tag}};
# these pass
is $sf->location->to_FTstring, $ftstring, 'Feature string';
is $sf->spliced_seq->subseq(1,10), $first_ten, 'First ten nucleotides';
is $sf->strand, $strand, 'Strand';
TODO: {
local $TODO = "Need to define how to deal with start, end length for circular sequences";
is $sf->start, $start, 'Start';
is $sf->end, $end, 'End';
is $sf->length, $length, 'Expected length';
}
}