BLASTP 2.2.23+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: subject.fa
           1 sequences; 311 total letters



Query=  query
Length=220
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  subject                                                              162    4e-45


> subject
Length=311

 Score =  162 bits (411),  Expect = 4e-45, Method: Compositional matrix adjust.
 Identities = 110/308 (35%), Positives = 145/308 (47%), Gaps = 94/308 (30%)

Query  5    NNTQISGFLLMGLSNKPELQLPIFGLFLSMYLITVFGNLLIILDISSDSHLHTPMYFF--  62
            N + IS F L G+S  PE Q  +FG+FL MYL+T+ GNLLIIL I SD HLHTPMYFF  
Sbjct  3    NQSSISEFFLRGISAPPEQQQSLFGIFLCMYLVTLTGNLLIILAIGSDLHLHTPMYFFLA  62

Query       ------------------------------------------------------------  
                                                                        
Sbjct  63   NLSFVDMGLTSSTVTKMLVNIQTRHHTISYTGCLTQMYFFLMFGDLDSFFLAAMAYDRYV  122

Query  63   --------------------------LANLXV--QSLMLLQLSFCSEVEIPHFFCELHQM  94
                                      L N+     + ++ +LSFC   EI HFFC++  +
Sbjct  123  AICHPLCYSTVMRPQVCALMLALCWVLTNIVALTHTFLMARLSFCVTGEIAHFFCDITPV  182

Query  95   IQLACSDTFLNDTVIYV--STVLLACGPLTGILYSYSKIVSSICRISSAQGKYKAFSTCA  152
            ++L+CSDT +N+ +++V   TVL+   P   I+ SY  IV +I R+ +  G  KAFSTC+
Sbjct  183  LKLSCSDTHINEMMVFVLGGTVLIV--PFLCIVTSYIHIVPAILRVRTRGGVGKAFSTCS  240

Query  153  SHLSVVSLFYCTVLGVYLSCAATQSSHGSAVASVMYTVVTPMLNPFIYSLRNKDIKEALI  212
            SHL VV +FY T+   YL   +  S      A+ MYT+VTPMLNPFIYSLRNKD+K AL 
Sbjct  241  SHLCVVCVFYGTLFSAYLCPPSIASEEKDIAAAAMYTIVTPMLNPFIYSLRNKDMKGALK  300

Query  213  RFLRRVTI  220
            R     +I
Sbjct  301  RLFSHRSI  308


 Score = 15.0 bits (27),  Expect = 1.9, Method: Compositional matrix adjust.
 Identities = 6/10 (60%), Positives = 8/10 (80%), Gaps = 0/10 (0%)

Query  125  LYSYSKIVSS  134
            L+S+  IVSS
Sbjct  302  LFSHRSIVSS  311



Lambda     K      H
   0.328    0.139    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 55770


  Database: subject.fa
    Posted date:  Apr 26, 2010  2:49 PM
  Number of letters in database: 311
  Number of sequences in database:  1



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40