#
# BioPerl module for Bio::DB::IndexedBase
#
# You may distribute this module under the same terms as perl itself
#
=head1 NAME
Bio::DB::IndexedBase - Base class for modules using indexed sequence files
=head1 SYNOPSIS
use Bio::DB::XXX; # a made-up class that uses Bio::IndexedBase
# 1/ Bio::SeqIO-style access
# Index some sequence files
my $db = Bio::DB::XXX->new('/path/to/file'); # from a single file
my $db = Bio::DB::XXX->new(['file1', 'file2']); # from multiple files
my $db = Bio::DB::XXX->new('/path/to/files/'); # from a directory
# Get IDs of all the sequences in the database
my @ids = $db->get_all_primary_ids;
# Get a specific sequence
my $seq = $db->get_Seq_by_id('CHROMOSOME_I');
# Loop through all sequences
my $stream = $db->get_PrimarySeq_stream;
while (my $seq = $stream->next_seq) {
# Do something...
}
# 2/ Access via filehandle
my $fh = Bio::DB::XXX->newFh('/path/to/file');
while (my $seq = <$fh>) {
# Do something...
}
# 3/ Tied-hash access
tie %sequences, 'Bio::DB::XXX', '/path/to/file';
print $sequences{'CHROMOSOME_I:1,20000'};
=head1 DESCRIPTION
Bio::DB::IndexedBase provides a base class for modules that want to index
and read sequence files and provides persistent, random access to each sequence
entry, without bringing the entire file into memory. This module is compliant
with the Bio::SeqI interface and both. Bio::DB::Fasta and Bio::DB::Qual both use
Bio::DB::IndexedBase.
When you initialize the module, you point it at a single file, several files, or
a directory of files. The first time it is run, the module generates an index
of the content of the files using the AnyDBM_File module (BerkeleyDB preferred,
followed by GDBM_File, NDBM_File, and SDBM_File). Subsequently, it uses the
index file to find the sequence file and offset for any requested sequence. If
one of the source files is updated, the module reindexes just that one file. You
can also force reindexing manually at any time. For improved performance, the
module keeps a cache of open filehandles, closing less-recently used ones when
the cache is full.
Entries may have any line length up to 65,536 characters, and different line
lengths are allowed in the same file. However, within a sequence entry, all
lines must be the same length except for the last. An error will be thrown if
this is not the case!
This module was developed for use with the C. elegans and human genomes, and has
been tested with sequence segments as large as 20 megabases. Indexing the C.
elegans genome (100 megabases of genomic sequence plus 100,000 ESTs) takes ~5
minutes on my 300 MHz pentium laptop. On the same system, average access time
for any 200-mer within the C. elegans genome was E<lt>0.02s.
=head1 DATABASE CREATION AND INDEXING
The two constructors for this class are new() and newFh(). The former creates a
Bio::DB::IndexedBase object which is accessed via method calls. The latter
creates a tied filehandle which can be used Bio::SeqIO style to fetch sequence
objects in a stream fashion. There is also a tied hash interface.
=over
=item $db = Bio::DB::IndexedBase-E<gt>new($path [,%options])
Create a new Bio::DB::IndexedBase object from the files designated by $path
$path may be a single file, an arrayref of files, or a directory containing
such files.
After the database is created, you can use methods like get_all_primary_ids()
and get_Seq_by_id() to retrieve sequence objects.
=item $fh = Bio::DB::IndexedBase-E<gt>newFh($path [,%options])
Create a tied filehandle opened on a Bio::DB::IndexedBase object. Reading
from this filehandle with E<lt>E<gt> will return a stream of sequence objects,
Bio::SeqIO style. The path and the options should be specified as for new().
=item $obj = tie %db,'Bio::DB::IndexedBase', '/path/to/file' [,@args]
Create a tied-hash by tieing %db to Bio::DB::IndexedBase using the indicated
path to the files. The optional @args list is the same set used by new(). If
successful, tie() returns the tied object, undef otherwise.
Once tied, you can use the hash to retrieve an individual sequence by
its ID, like this:
my $seq = $db{CHROMOSOME_I};
The keys() and values() functions will return the sequence IDs and their
sequences, respectively. In addition, each() can be used to iterate over the
entire data set:
while (my ($id,$sequence) = each %db) {
print "$id => $sequence\n";
}
When dealing with very large sequences, you can avoid bringing them into memory
by calling each() in a scalar context. This returns the key only. You can then
use tied(%db) to recover the Bio::DB::IndexedBase object and call its methods.
while (my $id = each %db) {
print "$id: $db{$sequence:1,100}\n";
print "$id: ".tied(%db)->length($id)."\n";
}
In addition, you may invoke the FIRSTKEY and NEXTKEY tied hash methods directly
to retrieve the first and next ID in the database, respectively. This allows to
write the following iterative loop using just the object-oriented interface:
my $db = Bio::DB::IndexedBase->new('/path/to/file');
for (my $id=$db->FIRSTKEY; $id; $id=$db->NEXTKEY($id)) {
# do something with sequence
}
=back
=head1 INDEX CONTENT
Several attributes of each sequence are stored in the index file. Given a
sequence ID, these attributes can be retrieved using the following methods:
=over
=item offset($id)
Get the offset of the indicated sequence from the beginning of the file in which
it is located. The offset points to the beginning of the sequence, not the
beginning of the header line.
=item strlen($id)
Get the number of characters in the sequence string.
=item length($id)
Get the number of residues of the sequence.
=item linelen($id)
Get the length of the line for this sequence. If the sequence is wrapped, then
linelen() is likely to be much shorter than strlen().
=item headerlen($id)
Get the length of the header line for the indicated sequence.
=item header_offset
Get the offset of the header line for the indicated sequence from the beginning
of the file in which it is located. This attribute is not stored. It is
calculated from offset() and headerlen().
=item alphabet($id)
Get the molecular type (alphabet) of the indicated sequence. This method handles
residues according to the IUPAC convention.
=item file($id)
Get the the name of the file in which the indicated sequence can be found.
=back
=head1 INTERFACE COMPLIANCE NOTES
Bio::DB::IndexedBase is compliant with the Bio::DB::SeqI and hence with the
Bio::RandomAccessI interfaces.
Database do not necessarily provide any meaningful internal primary ID for the
sequences they store. However, Bio::DB::IndexedBase's internal primary IDs are
the IDs of the sequences. This means that the same ID passed to get_Seq_by_id()
and get_Seq_by_primary_id() will return the same sequence.
Since this database index has no notion of sequence version or namespace, the
get_Seq_by_id(), get_Seq_by_acc() and get_Seq_by_version() are identical.
=head1 BUGS
When a sequence is deleted from one of the files, this deletion is not detected
by the module and removed from the index. As a result, a "ghost" entry will
remain in the index and will return garbage results if accessed.
Also, if you are indexing a directory, it is wise to not add or remove files
from it.
In case you have changed the files in a directory, or the sequences in a file,
you can to rebuild the entire index, either by deleting it manually, or by
passing -reindex=E<gt>1 to new() when initializing the module.
=head1 SEE ALSO
L<DB_File>
L<Bio::DB::Fasta>
L<Bio::DB::Qual>
=head1 AUTHOR
Lincoln Stein E<lt>lstein@cshl.orgE<gt>.
Copyright (c) 2001 Cold Spring Harbor Laboratory.
Florent Angly (for the modularization)
This library is free software; you can redistribute it and/or modify
it under the same terms as Perl itself. See DISCLAIMER.txt for
disclaimers of warranty.
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
package Bio::DB::IndexedBase;
BEGIN {
@AnyDBM_File::ISA = qw(DB_File GDBM_File NDBM_File SDBM_File)
if(!$INC{'AnyDBM_File.pm'});
}
use strict;
use IO::File;
use AnyDBM_File;
use Fcntl;
use File::Spec;
use File::Basename qw(basename dirname);
use Bio::PrimarySeq;
use base qw(Bio::DB::SeqI);
# Store offset, strlen, linelen, headerlen, type and fileno
use constant STRUCT => 'NNNnnCa*'; # 32-bit file offset and seq length
use constant STRUCTBIG => 'QQQnnCa*'; # 64-bit
use constant NA => 0;
use constant DNA => 1;
use constant RNA => 2;
use constant PROTEIN => 3;
use constant DIE_ON_MISSMATCHED_LINES => 1;
# you can avoid dying if you want but you may get incorrect results
=head2 new
Title : new
Usage : my $db = Bio::DB::IndexedBase->new($path, -reindex => 1);
Function: Initialize a new database object
Returns : A Bio::DB::IndexedBase object
Args : A single file, or path to dir, or arrayref of files
Optional arguments:
Option Description Default
----------- ----------- -------
-glob Glob expression to search for files in directories *
-makeid A code subroutine for transforming IDs None
-maxopen Maximum size of filehandle cache 32
-debug Turn on status messages 0
-reindex Force the index to be rebuilt 0
-dbmargs Additional arguments to pass to the DBM routine None
-index_name Name of the file that will hold the indices
-clean Remove the index file when finished 0
The -dbmargs option can be used to control the format of the index. For example,
you can pass $DB_BTREE to this argument so as to force the IDs to be sorted and
retrieved alphabetically. Note that you must use the same arguments every time
you open the index!
The -makeid option gives you a chance to modify sequence IDs during indexing.
For example, you may wish to extract a portion of the gi|gb|abc|xyz nonsense
that GenBank Fasta files use. The original header line can be recovered later.
The option value for -makeid should be a code reference that takes a scalar
argument (the full header line) and returns a scalar or an array of scalars (the
ID or IDs you want to assign). For example:
$db = Bio::DB::IndexedBase->new('file.fa', -makeid => \&extract_gi);
sub extract_gi {
# Extract GI from GenBank
my $header = shift;
my ($id) = ($header =~ /gi\|(\d+)/m);
return $id || '';
}
extract_gi() will be called with the full header line, e.g. a Fasta line would
include the "E<gt>", the ID and the description:
>gi|352962132|ref|NG_030353.1| Homo sapiens sal-like 3 (Drosophila) (SALL3)
In the database, this sequence can now be retrieved by its GI instead of its
complete ID:
my $seq = $db->get_Seq_by_id(352962132);
The -makeid option is ignored after the index is constructed.
=cut
sub new {
my ($class, $path, %opts) = @_;
my $self = bless {
debug => $opts{-debug} || 0,
makeid => $opts{-makeid},
glob => $opts{-glob} || eval '$'.$class.'::file_glob' || '*',
maxopen => $opts{-maxopen} || 32,
clean => $opts{-clean} || 0,
dbmargs => $opts{-dbmargs} || undef,
fhcache => {},
cacheseq => {},
curopen => 0,
openseq => 1,
dirname => undef,
offsets => undef,
index_name => $opts{-index_name},
obj_class => eval '$'.$class.'::obj_class',
offset_meth => \&{$class.'::_calculate_offsets'},
fileno2path => [],
filepath2no => {},
}, $class;
my ($offsets, $dirname);
my $ref = ref $path || '';
if ( $ref eq 'ARRAY' ) {
$offsets = $self->index_files($path, $opts{-reindex});
require Cwd;
$dirname = Cwd::getcwd();
} else {
if (-d $path) {
# because Win32 glob() is broken with respect to long file names
# that contain whitespace.
$path = Win32::GetShortPathName($path)
if $^O =~ /^MSWin/i && eval 'use Win32; 1';
$offsets = $self->index_dir($path, $opts{-reindex});
$dirname = $path;
} elsif (-f _) {
$offsets = $self->index_file($path, $opts{-reindex});
$dirname = dirname($path);
} else {
$self->throw( "No file or directory called '$path'");
}
}
@{$self}{qw(dirname offsets)} = ($dirname, $offsets);
return $self;
}
=head2 newFh
Title : newFh
Usage : my $fh = Bio::DB::IndexedBase->newFh('/path/to/files/', %options);
Function: Index and get a new Fh for a single file, several files or a directory
Returns : Filehandle object
Args : Same as new()
=cut
sub newFh {
my ($class, @args) = @_;
my $self = $class->new(@args);
require Symbol;
my $fh = Symbol::gensym;
tie $$fh, 'Bio::DB::Indexed::Stream', $self
or $self->throw("Could not tie filehandle: $!");
return $fh;
}
=head2 dbmargs
Title : dbmargs
Usage : my @args = $db->dbmargs;
Function: Get stored dbm arguments
Returns : Array
Args : None
=cut
sub dbmargs {
my $self = shift;
my $args = $self->{dbmargs} or return;
return ref($args) eq 'ARRAY' ? @$args : $args;
}
=head2 glob
Title : glob
Usage : my $glob = $db->glob;
Function: Get the expression used to match files in directories
Returns : String
Args : None
=cut
sub glob {
my $self = shift;
return $self->{glob};
}
=head2 index_dir
Title : index_dir
Usage : $db->index_dir($dir);
Function: Index the files that match -glob in the given directory
Returns : Hashref of offsets
Args : Dirname
Boolean to force a reindexing the directory
=cut
sub index_dir {
my ($self, $dir, $force_reindex) = @_;
my @files = glob( File::Spec->catfile($dir, $self->{glob}) );
$self->throw("No suitable files found in $dir") if scalar @files == 0;
$self->{index_name} ||= File::Spec->catfile($dir, 'directory.index');
my $offsets = $self->_index_files(\@files, $force_reindex);
return $offsets;
}
=head2 get_all_primary_ids
Title : get_all_primary_ids, get_all_ids, ids
Usage : my @ids = $db->get_all_primary_ids;
Function: Get the IDs stored in all indexes. This is a Bio::DB::SeqI method
implementation. Note that in this implementation, the internal
database primary IDs are also the sequence IDs.
Returns : List of ids
Args : None
=cut
sub get_all_primary_ids {
return keys %{shift->{offsets}};
}
*ids = *get_all_ids = \&get_all_primary_ids;
=head2 index_file
Title : index_file
Usage : $db->index_file($filename);
Function: Index the given file
Returns : Hashref of offsets
Args : Filename
Boolean to force reindexing the file
=cut
sub index_file {
my ($self, $file, $force_reindex) = @_;
$self->{index_name} ||= "$file.index";
my $offsets = $self->_index_files([$file], $force_reindex);
return $offsets;
}
=head2 index_files
Title : index_files
Usage : $db->index_files(\@files);
Function: Index the given files
Returns : Hashref of offsets
Args : Arrayref of filenames
Boolean to force reindexing the files
=cut
sub index_files {
my ($self, $files, $force_reindex) = @_;
my @paths = map { File::Spec->rel2abs($_) } @$files;
require Digest::MD5;
my $digest = Digest::MD5::md5_hex( join('', sort @paths) );
$self->{index_name} ||= "fileset_$digest.index"; # unique name for the given files
my $offsets = $self->_index_files($files, $force_reindex);
return $offsets;
}
=head2 index_name
Title : index_name
Usage : my $indexname = $db->index_name($path);
Function: Get the full name of the index file
Returns : String
Args : None
=cut
sub index_name {
return shift->{index_name};
}
=head2 path
Title : path
Usage : my $path = $db->path($path);
Function: When a single file or a directory of files is indexed, this returns
the file directory. When indexing an arbitrary list of files, the
return value is the path of the current working directory.
Returns : String
Args : None
=cut
sub path {
return shift->{dirname};
}
=head2 get_PrimarySeq_stream
Title : get_PrimarySeq_stream
Usage : my $stream = $db->get_PrimarySeq_stream();
Function: Get a SeqIO-like stream of sequence objects. The stream supports a
single method, next_seq(). Each call to next_seq() returns a new
PrimarySeqI compliant sequence object, until no more sequences remain.
This is a Bio::DB::SeqI method implementation.
Returns : A Bio::DB::Indexed::Stream object
Args : None
=cut
sub get_PrimarySeq_stream {
my $self = shift;
return Bio::DB::Indexed::Stream->new($self);
}
=head2 get_Seq_by_id
Title : get_Seq_by_id, get_Seq_by_acc, get_Seq_by_version, get_Seq_by_primary_id
Usage : my $seq = $db->get_Seq_by_id($id);
Function: Given an ID, fetch the corresponding sequence from the database.
This is a Bio::DB::SeqI and Bio::DB::RandomAccessI method implementation.
Returns : A sequence object
Args : ID
=cut
sub get_Seq_by_id {
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
return if not exists $self->{offsets}{$id};
return $self->{obj_class}->new($self, $id);
}
*get_Seq_by_version = *get_Seq_by_primary_id = *get_Seq_by_acc = \&get_Seq_by_id;
=head2 _calculate_offsets
Title : _calculate_offsets
Usage : $db->_calculate_offsets($filename, $offsets);
Function: This method calculates the sequence offsets in a file based on ID and
should be implemented by classes that use Bio::DB::IndexedBase.
Returns : Hash of offsets
Args : File to process
Hashref of file offsets keyed by IDs.
=cut
sub _calculate_offsets {
my $self = shift;
$self->throw_not_implemented();
}
sub _index_files {
# Do the indexing of the given files using the index file on record
my ($self, $files, $force_reindex) = @_;
$self->_set_pack_method( @$files );
# Get name of index file
my $index = $self->index_name;
# If caller has requested reindexing, unlink the index file.
unlink $index if $force_reindex;
# Get the modification time of the index
my $indextime = (stat $index)[9] || 0;
# Register files and find if there has been any update
my $modtime = 0;
my @updated;
for my $file (@$files) {
# Register file
$self->_path2fileno(basename($file));
# Any update?
my $m = (stat $file)[9] || 0;
if ($m > $modtime) {
$modtime = $m;
}
if ($m > $indextime) {
push @updated, $file;
}
}
# Get termination length from first file
$self->{termination_length} = $self->_calc_termination_length( $files->[0] );
# Reindex contents of changed files if needed
my $reindex = $force_reindex || (scalar @updated > 0);
$self->{offsets} = $self->_open_index($index, $reindex) or return;
if ($reindex) {
$self->{indexing} = $index;
for my $file (@updated) {
my $fileno = $self->_path2fileno(basename($file));
&{$self->{offset_meth}}($self, $fileno, $file, $self->{offsets});
}
delete $self->{indexing};
}
# Closing and reopening might help corrupted index file problem on Windows
$self->_close_index($self->{offsets});
return $self->{offsets} = $self->_open_index($index);
}
sub _open_index {
# Open index file in read-only or write mode
my ($self, $index_file, $write) = @_;
my %offsets;
my $flags = $write ? O_CREAT|O_RDWR : O_RDONLY;
my @dbmargs = $self->dbmargs;
tie %offsets, 'AnyDBM_File', $index_file, $flags, 0644, @dbmargs
or $self->throw( "Could not open index file $index_file: $!");
return \%offsets;
}
sub _close_index {
# Close index file
my ($self, $index) = @_;
untie %$index;
return 1;
}
sub _parse_compound_id {
# Handle compound IDs:
# $db->seq($id)
# $db->seq($id, $start, $stop, $strand)
# $db->seq("$id:$start,$stop")
# $db->seq("$id:$start..$stop")
# $db->seq("$id:$start-$stop")
# $db->seq("$id:$start,$stop/$strand")
# $db->seq("$id:$start..$stop/$strand")
# $db->seq("$id:$start-$stop/$strand")
# $db->seq("$id/$strand")
my ($self, $id, $start, $stop, $strand) = @_;
if ( (not defined $start ) &&
(not defined $stop ) &&
(not defined $strand) &&
($id =~ /^ (.+?) (?:\:([\d_]+)(?:,|-|\.\.)([\d_]+))? (?:\/(.+))? $/x) ) {
# Start, stop and strand not provided and ID looks like a compound ID
($id, $start, $stop, $strand) = ($1, $2, $3, $4);
}
# Start, stop and strand defaults
$stop ||= $self->length($id) || 0; # 0 if sequence not found in database
$start ||= ($stop > 0) ? 1 : 0;
$strand ||= 1;
# Convert numbers such as 1_000_000 to 1000000
$start =~ s/_//g;
$stop =~ s/_//g;
if ($start > $stop) {
# Change the strand
($start, $stop) = ($stop, $start);
$strand *= -1;
}
return $id, $start, $stop, $strand;
}
sub _guess_alphabet {
# Determine the molecular type of the given sequence string:
# 'dna', 'rna', 'protein' or '' (unknown/empty)
my ($self, $string) = @_;
# Handle IUPAC residues like PrimarySeq does
my $alphabet = Bio::PrimarySeq::_guess_alphabet_from_string($self, $string, 1);
return $alphabet eq 'dna' ? DNA
: $alphabet eq 'rna' ? RNA
: $alphabet eq 'protein' ? PROTEIN
: NA;
}
sub _makeid {
# Process the header line by applying any transformation given in -makeid
my ($self, $header_line) = @_;
return ref($self->{makeid}) eq 'CODE' ? $self->{makeid}->($header_line) : $1;
}
sub _check_linelength {
# Check that the line length is valid. Generate an error otherwise.
my ($self, $linelength) = @_;
return if not defined $linelength;
$self->throw(
"Each line of the qual file must be less than 65,536 characters. Line ".
"$. is $linelength chars."
) if $linelength > 65535;
}
sub _calc_termination_length {
# Try the beginning of the file to determine termination length
# Account for crlf-terminated Windows and Mac files
my ($self, $file) = @_;
my $fh = IO::File->new($file) or $self->throw( "Could not open $file: $!");
# In Windows, text files have '\r\n' as line separator, but when reading in
# text mode Perl will only show the '\n'. This means that for a line "ABC\r\n",
# "length $_" will report 4 although the line is 5 bytes in length.
# We assume that all lines have the same line separator and only read current line.
my $init_pos = tell($fh);
my $curr_line = <$fh>;
my $pos_diff = tell($fh) - $init_pos;
my $correction = $pos_diff - length $curr_line;
close $fh;
$self->{termination_length} = ($curr_line =~ /\r\n$/) ? 2 : 1+$correction;
return $self->{termination_length};
}
sub _calc_offset {
# Get the offset of the n-th residue of the sequence with the given ID
# and termination length (tl)
my ($self, $id, $n) = @_;
my $tl = $self->{termination_length};
$n--;
my ($offset, $seqlen, $linelen) = (&{$self->{unpackmeth}}($self->{offsets}{$id}))[0,1,3];
$n = 0 if $n < 0;
$n = $seqlen-1 if $n >= $seqlen;
return $offset + $linelen * int($n/($linelen-$tl)) + $n % ($linelen-$tl);
}
sub _fh {
# Given a sequence ID, return the filehandle on which to find this sequence
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
my $file = $self->file($id) or return;
return $self->_fhcache( File::Spec->catfile($self->{dirname}, $file) ) or
$self->throw( "Can't open file $file");
}
sub _fhcache {
my ($self, $path) = @_;
if (!$self->{fhcache}{$path}) {
if ($self->{curopen} >= $self->{maxopen}) {
my @lru = sort {$self->{cacheseq}{$a} <=> $self->{cacheseq}{$b};}
keys %{$self->{fhcache}};
splice(@lru, $self->{maxopen} / 3);
$self->{curopen} -= @lru;
for (@lru) {
delete $self->{fhcache}{$_};
}
}
$self->{fhcache}{$path} = IO::File->new($path) || return;
binmode $self->{fhcache}{$path};
$self->{curopen}++;
}
$self->{cacheseq}{$path}++;
return $self->{fhcache}{$path};
}
#-------------------------------------------------------------
# Methods to store and retrieve data from indexed file
#
=head2 offset
Title : offset
Usage : my $offset = $db->offset($id);
Function: Get the offset of the indicated sequence from the beginning of the
file in which it is located. The offset points to the beginning of
the sequence, not the beginning of the header line.
Returns : String
Args : ID of sequence
=cut
sub offset {
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
my $offset = $self->{offsets}{$id} or return;
return (&{$self->{unpackmeth}}($offset))[0];
}
=head2 strlen
Title : strlen
Usage : my $length = $db->strlen($id);
Function: Get the number of characters in the sequence string.
Returns : Integer
Args : ID of sequence
=cut
sub strlen {
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
my $offset = $self->{offsets}{$id} or return;
return (&{$self->{unpackmeth}}($offset))[1];
}
=head2 length
Title : length
Usage : my $length = $db->length($id);
Function: Get the number of residues of the sequence.
Returns : Integer
Args : ID of sequence
=cut
sub length {
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
my $offset = $self->{offsets}{$id} or return;
return (&{$self->{unpackmeth}}($offset))[2];
}
=head2 linelen
Title : linelen
Usage : my $linelen = $db->linelen($id);
Function: Get the length of the line for this sequence.
Returns : Integer
Args : ID of sequence
=cut
sub linelen {
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
my $offset = $self->{offsets}{$id} or return;
return (&{$self->{unpackmeth}}($offset))[3];
}
=head2 headerlen
Title : headerlen
Usage : my $length = $db->headerlen($id);
Function: Get the length of the header line for the indicated sequence.
Returns : Integer
Args : ID of sequence
=cut
sub headerlen {
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
my $offset = $self->{offsets}{$id} or return;
return (&{$self->{unpackmeth}}($offset))[4];
}
=head2 header_offset
Title : header_offset
Usage : my $offset = $db->header_offset($id);
Function: Get the offset of the header line for the indicated sequence from
the beginning of the file in which it is located.
Returns : String
Args : ID of sequence
=cut
sub header_offset {
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
return if not $self->{offsets}{$id};
return $self->offset($id) - $self->headerlen($id);
}
=head2 alphabet
Title : alphabet
Usage : my $alphabet = $db->alphabet($id);
Function: Get the molecular type of the indicated sequence: dna, rna or protein
Returns : String
Args : ID of sequence
=cut
sub alphabet {
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
my $offset = $self->{offsets}{$id} or return;
my $alphabet = (&{$self->{unpackmeth}}($offset))[5];
return : $alphabet == Bio::DB::IndexedBase::DNA ? 'dna'
: $alphabet == Bio::DB::IndexedBase::RNA ? 'rna'
: $alphabet == Bio::DB::IndexedBase::PROTEIN ? 'protein'
: '';
}
=head2 file
Title : file
Usage : my $file = $db->file($id);
Function: Get the the name of the file in which the indicated sequence can be
found.
Returns : String
Args : ID of sequence
=cut
sub file {
my ($self, $id) = @_;
$self->throw('Need to provide a sequence ID') if not defined $id;
my $offset = $self->{offsets}{$id} or return;
return $self->_fileno2path((&{$self->{unpackmeth}}($offset))[6]);
}
sub _fileno2path {
my ($self, $fileno) = @_;
return $self->{fileno2path}->[$fileno];
}
sub _path2fileno {
my ($self, $path) = @_;
if ( not exists $self->{filepath2no}->{$path} ) {
my $fileno = ($self->{filepath2no}->{$path} = 0+ $self->{fileno}++);
$self->{fileno2path}->[$fileno] = $path; # Save path
}
return $self->{filepath2no}->{$path};
}
sub _packSmall {
return pack STRUCT, @_;
}
sub _packBig {
return pack STRUCTBIG, @_;
}
sub _unpackSmall {
return unpack STRUCT, shift;
}
sub _unpackBig {
return unpack STRUCTBIG, shift;
}
sub _set_pack_method {
# Determine whether to use 32 or 64 bit integers for the given files.
my $self = shift;
# Find the maximum file size:
my ($maxsize) = sort { $b <=> $a } map { -s $_ } @_;
my $fourGB = (2 ** 32) - 1;
if ($maxsize > $fourGB) {
# At least one file exceeds 4Gb - we will need to use 64 bit ints
$self->{packmeth} = \&_packBig;
$self->{unpackmeth} = \&_unpackBig;
} else {
$self->{packmeth} = \&_packSmall;
$self->{unpackmeth} = \&_unpackSmall;
}
return 1;
}
#-------------------------------------------------------------
# Tied hash logic
#
sub TIEHASH {
return shift->new(@_);
}
sub FETCH {
return shift->subseq(@_);
}
sub STORE {
shift->throw("Read-only database");
}
sub DELETE {
shift->throw("Read-only database");
}
sub CLEAR {
shift->throw("Read-only database");
}
sub EXISTS {
return defined shift->offset(@_);
}
sub FIRSTKEY {
return tied(%{shift->{offsets}})->FIRSTKEY(@_);
}
sub NEXTKEY {
return tied(%{shift->{offsets}})->NEXTKEY(@_);
}
sub DESTROY {
my $self = shift;
# Close filehandles
while (my ($file, $fh) = each %{ $self->{fhcache} }) {
if (defined $fh) {
$fh->close;
}
}
$self->_close_index($self->{offsets});
if ( $self->{clean} || $self->{indexing} ) {
# Indexing aborted or cleaning requested. Delete the index file.
unlink $self->{index_name};
}
return 1;
}
#-------------------------------------------------------------
# stream-based access to the database
#
package Bio::DB::Indexed::Stream;
use base qw(Tie::Handle Bio::DB::SeqI);
sub new {
my ($class, $db) = @_;
my $key = $db->FIRSTKEY;
return bless {
db => $db,
key => $key
}, $class;
}
sub next_seq {
my $self = shift;
my ($key, $db) = @{$self}{'key', 'db'};
return if not defined $key;
my $value = $db->get_Seq_by_id($key);
$self->{key} = $db->NEXTKEY($key);
return $value;
}
sub TIEHANDLE {
my ($class, $db) = @_;
return $class->new($db);
}
sub READLINE {
my $self = shift;
return $self->next_seq;
}
1;