BLASTP 2.0MP-WashU [12-Feb-2001] [linux-i686 01:36:08 31-Jan-2001]
Copyright (C) 1996-2000 Washington University, Saint Louis, Missouri USA.
All Rights Reserved.
Reference: Gish, W. (1996-2000) http://blast.wustl.edu
Query= gi|1786183|gb|AAC73113.1| (AE000111) aspartokinase I, homoserine
dehydrogenase I [Escherichia coli]
(820 letters)
Database: ecoli.aa
4289 sequences; 1,358,990 total letters.
Searching....10....20....30....40....50....60....70....80....90....100% done
Smallest
Sum
High Probability
Sequences producing High-scoring Segment Pairs: Score P(N) N
gb|AAC73113.1| (AE000111) aspartokinase I, homoserine deh... 4141 0.0 1
gb|AAC76922.1| (AE000468) aspartokinase II and homoserine... 844 3.1e-86 1
gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensi... 483 2.8e-47 1
gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia c... 97 0.0010 1
>gb|AAC73113.1| (AE000111) aspartokinase I, homoserine dehydrogenase I
[Escherichia coli]
Length = 820
Score = 4141 (1462.8 bits), Expect = 0.0, P = 0.0
Identities = 820/820 (100%), Positives = 820/820 (100%)
Query: 1 MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60
MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA
Sbjct: 1 MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDA 60
Query: 61 LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120
LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA
Sbjct: 61 LPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDSINA 120
Query: 121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180
ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP
Sbjct: 121 ALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRIP 180
Query: 181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV
Sbjct: 181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
Query: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD
Sbjct: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
Query: 301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF
Sbjct: 301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
Query: 361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420
CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL
Sbjct: 361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAAL 420
Query: 421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL 480
ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL
Sbjct: 421 ARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGAL 480
Query: 481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540
LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL
Sbjct: 481 LEQLKRQQSWLKNKHIDLRVCGVANSKALLTNVHGLNLENWQEELAQAKEPFNLGRLIRL 540
Query: 541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600
VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR
Sbjct: 541 VKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSR 600
Query: 601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660
RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA
Sbjct: 601 RKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLA 660
Query: 661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720
REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA
Sbjct: 661 REMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMA 720
Query: 721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780
NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF
Sbjct: 721 NLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAF 780
Query: 781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820
YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV
Sbjct: 781 YSHYYQPLPLVLRGYGAGNDVTAAGVFADLLRTLSWKLGV 820
>gb|AAC76922.1| (AE000468) aspartokinase II and homoserine dehydrogenase II
[Escherichia coli]
Length = 810
Score = 844 (302.2 bits), Expect = 3.1e-86, P = 3.1e-86
Identities = 222/705 (31%), Positives = 356/705 (50%)
Query: 116 DSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIA 175
D++ A ++ GE S +M+ VL +G +D E L A + VD S +
Sbjct: 119 DAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE-RAAQPQVDEGLSYPLLQ 177
Query: 176 ASRI--PADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVY 233
+ P +V+ GF + N GE V+LGRNGSDYSA + A IW+DV GVY
Sbjct: 178 QLLVQHPGKRLVV-TGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVY 236
Query: 234 TCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGT 293
+ DPR+V DA LL + EA EL+ A VLH RT+ P++ +I ++ + P T
Sbjct: 237 SADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGST 296
Query: 294 LIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSS 353
I + +++ +++ + P + + + RA++ + + +
Sbjct: 297 RIERVLASGT-GARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHN 355
Query: 354 SEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGIS 413
+ FC A + + E GL L + + LA++++VG G+ T +
Sbjct: 356 DRQLLQFCYTSEVADSALKILDEA-------GLPGELRLRQGLALVAMVGAGV-TRNPLH 407
Query: 414 A-KFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIG 472
+F+ L + Q S+ V+ + ++ HQ +F ++ I + + G
Sbjct: 408 CHRFWQQLKGQPVEFTW--QSDDGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFG 465
Query: 473 VGGVGGALLEQLKRQQSWLKNKH-IDLRVCGVANSKALLTNVHGLN----LENWQEELAQ 527
G +G LE R+QS L + + + GV +S+ L + GL+ L + +E +
Sbjct: 466 KGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVE 525
Query: 528 AKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYADFLREGFHVVTPNKKANTSSMD 587
E L ++ + + V++D T+SQ +ADQY DF GFHV++ NK A S +
Sbjct: 526 QDEE----SLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGFHVISANKLAGASDSN 581
Query: 588 YYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLNAGDELMKFSGILSGSLSYIFGKL 647
Y Q+ A EK+ R +LY+ VGAGLP+ +++L+++GD ++ SGI SG+LS++F +
Sbjct: 582 KYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTILSISGIFSGTLSWLFLQF 641
Query: 648 DEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARKLLILARETGRELELADIEIEPVLPA 707
D + F+E A + G TEPDPRDDLSG DV RKL+ILARE G +E + +E ++PA
Sbjct: 642 DGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAREAGYNIEPDQVRVESLVPA 701
Query: 708 EFNAEGDVAAFMANLSQLDDLFAARVAKARDEGKVLRYVGNIDEDGVCRVKIAEVDGNDP 767
G + F N +L++ R+ AR+ G VLRYV D +G RV + V + P
Sbjct: 702 HCEG-GSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVARFDANGKARVGVEAVREDHP 760
Query: 768 LFKVKNGENALAFYSHYYQPLPLVLRGYGAGNDVTAAGVFADLLR 812
L + +N A S +Y+ PLV+RG GAG DVTA + +D+ R
Sbjct: 761 LASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGAIQSDINR 805
Score = 321 (118.1 bits), Expect = 3.6e-27, P = 3.6e-27
Identities = 108/406 (26%), Positives = 191/406 (47%)
Query: 5 KFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQDALPNI 64
KFGG+S+A+ + +LRVA I+ ++ + V+SA TN L+ ++ + + + + +
Sbjct: 16 KFGGSSLADVKCYLRVAGIMAEYSQPDDMM-VVSAAGSTTNQLINWLKLSQTDRLSAHQV 74
Query: 65 SDAERIF-AELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLH-GISLLGQCPDSINAAL 122
R + +L++GL A+ L + FV + ++ +L GI+ D++ A +
Sbjct: 75 QQTLRRYQCDLISGLLPAEEADSL--ISAFVS-DLERLAALLDSGIN------DAVYAEV 125
Query: 123 ICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHYLESTVDIAESTRRIAASRI--P 180
+ GE S +M+ VL +G +D E L A + VD S + + P
Sbjct: 126 VGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE-RAAQPQVDEGLSYPLLQQLLVQHP 184
Query: 181 ADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYTCDPRQV 240
+V+ GF + N GE V+LGRNGSDYSA + A IW+DV GVY+ DPR+V
Sbjct: 185 GKRLVV-TGFISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPRKV 243
Query: 241 PDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTLIGASRD 300
DA LL + EA EL+ A VLH RT+ P++ +I ++ + P T I
Sbjct: 244 KDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLA 303
Query: 301 EDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSSEYSISF 360
+ +++ +++ + P + + + RA++ + + + + F
Sbjct: 304 SGT-GARIVTSHDDVCLIEFQVPASQDFKLAHKEIDQILKRAQVRPLAVGVHNDRQLLQF 362
Query: 361 CVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGM 406
C A + + E GL L + + LA++++VG G+
Sbjct: 363 CYTSEVADSALKILDEA-------GLPGELRLRQGLALVAMVGAGV 401
>gb|AAC76994.1| (AE000475) aspartokinase III, lysine sensitive [Escherichia
coli]
Length = 449
Score = 483 (175.1 bits), Expect = 2.8e-47, P = 2.8e-47
Identities = 149/467 (31%), Positives = 233/467 (49%)
Query: 3 VLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKITNHLVAMIEKTISGQ---- 58
V KFGGTSVA+ + R ADI+ S+A V VLSA A ITN LVA+ E G+
Sbjct: 6 VSKFGGTSVADFDAMNRSADIVLSDANVRLV--VLSASAGITNLLVALAEGLEPGERFEK 63
Query: 59 -DALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIKHVLHGISLLGQCPDS 117
DA+ NI FA +L L +P ++ +++ I VL + L P +
Sbjct: 64 LDAIRNIQ-----FA-ILERLR-----YPNV-IREEIERLLENIT-VLAEAAALATSP-A 109
Query: 118 INAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVG-HYLESTVDIAESTRRIAA 176
+ L+ GE MS + +L R D V K++ + + DIA A
Sbjct: 110 LTDELVSHGELMSTLLFVEILRERDVQAQWFD-VRKVMRTNDRFGRAEPDIAALAELAAL 168
Query: 177 SRIPA--DHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYT 234
+P + +V+ GF KG LGR GSDY+AA+LA L A +IWTDV G+YT
Sbjct: 169 QLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVDIWTDVPGIYT 228
Query: 235 CDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQAPGTL 294
DPR V A+ + +++ EA E++ FGAKVLHP T+ P + IP + ++ +P+A GTL
Sbjct: 229 TDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPRAGGTL 288
Query: 295 IGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAARVFAAMSRARISVVLITQSSS 354
+ ++ E+ + ++ N + ++ M G A VF ++R ISV LIT +S
Sbjct: 289 V-CNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLIT--TS 345
Query: 355 EYSISFCVPQSDCVRA-ERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGIS 413
E S++ + + + + + +EL L + V E LA+++++G+ + G+
Sbjct: 346 EVSVALTLDTTGSTSTGDTLLTQSLLMELSA--LCRVEVEEGLALVALIGNDLSKACGVG 403
Query: 414 AKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLF 460
+ F L NI + I G+S ++ +V +DA V+ H LF
Sbjct: 404 KEVFGVLEPFNIRM--ICYGASSHNLCFLVPGEDAEQVVQKLHSNLF 448
>gb|AAC73282.1| (AE000126) uridylate kinase [Escherichia coli]
Length = 241
Score = 97 (39.2 bits), Expect = 0.0010, P = 0.0010
Identities = 30/98 (30%), Positives = 45/98 (45%)
Query: 199 LVVLGRN-GSDYSAAVLAACLR-----ADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQ 252
+V+L G+ + AACLR AD T VDGV+T DP + P A + + ++Y
Sbjct: 131 VVILSAGTGNPFFTTDSAACLRGIEIEADVVLKATKVDGVFTADPAKDPTATMYEQLTYS 190
Query: 253 EAMELSYFGAKVLHPRTITPIAQFQIPCLIKNTGNPQA 290
E +E KV+ T ++P + N P A
Sbjct: 191 EVLEKEL---KVMDLAAFTLARDHKLPIRVFNMNKPGA 225
Parameters:
E=0.01
ctxfactor=1.00
Query ----- As Used ----- ----- Computed ----
Frame MatID Matrix name Lambda K H Lambda K H
+0 0 BLOSUM62 0.319 0.136 0.384 same same same
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
Query
Frame MatID Length Eff.Length E S W T X E2 S2
+0 0 820 820 0.010 93 3 11 22 0.19 34
37 0.22 37
Statistics:
Database: /home/jes12/db/ecoli.aa
Title: ecoli.aa
Posted: 2:52:35 PM EST Nov 18, 2001
Created: 9:46:47 AM EST Nov 18, 2001
Format: XDF-1
# of letters in database: 1,358,990
# of sequences in database: 4289
# of database sequences satisfying E: 4
No. of states in DFA: 573 (61 KB)
Total size of DFA: 281 KB (1149 KB)
Time to generate neighborhood: 0.00u 0.02s 0.02t Elapsed: 00:00:00
No. of threads or processors used: 1
Search cpu time: 1.58u 0.00s 1.58t Elapsed: 00:00:01
Total cpu time: 1.59u 0.02s 1.61t Elapsed: 00:00:01
Start: Thu Dec 6 11:09:14 2001 End: Thu Dec 6 11:09:15 2001