TBLASTN 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= HAHU | 1114 | Hemoglobin alpha chain - Human, chimpanzee, and
pygmy chimpanzee
(141 letters)
Database: testnt.fa
2 sequences; 13,260 total letters
Searching..done
Score E
Sequences producing significant alignments: (bits) Value
gi|10040111|emb|AL390796.6|AL390796 Homo sapiens chromosome 1 cl... 18 2.3
test6 16 6.7
>gi|10040111|emb|AL390796.6|AL390796 Homo sapiens chromosome 1 clone
RP11-562F3, *** SEQUENCING IN PROGRESS ***, 16 unordered
pieces /len=164198
Length = 8160
Score = 17.7 bits (34), Expect = 2.3
Identities = 8/23 (34%), Positives = 11/23 (47%)
Frame = +1
Query: 56 KGHGKKVADALTNAVAHVDDMPN 78
KGH KK+ N ++ D N
Sbjct: 7603 KGHLKKITSFFFNHTSNYRDEYN 7671
Score = 17.3 bits (33), Expect = 3.0
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = +1
Query: 31 RMFLSFPTTKTYFPHFDLSHGSAQVKGH 58
RM+ F TY+ +F L V G+
Sbjct: 7069 RMYFCFLKVLTYYFNFFLFFNIFYVLGY 7152
>test6
Length = 5100
Score = 16.2 bits (30), Expect = 6.7
Identities = 6/9 (66%), Positives = 7/9 (77%)
Frame = +3
Query: 72 HVDDMPNAL 80
H+D M NAL
Sbjct: 3822 HMDYMSNAL 3848
Score = 15.8 bits (29), Expect = 8.8
Identities = 5/7 (71%), Positives = 6/7 (85%)
Frame = -2
Query: 93 VDPVNFK 99
+DP NFK
Sbjct: 1814 IDP*NFK 1794
Database: testnt.fa
Posted date: Feb 5, 2005 6:26 PM
Number of letters in database: 13,260
Number of sequences in database: 2
Lambda K H
0.319 0.130 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1998
Number of Sequences: 2
Number of extensions: 23
Number of successful extensions: 4
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4
length of query: 141
length of database: 4420
effective HSP length: 39
effective length of query: 102
effective length of database: 4342
effective search space: 442884
effective search space used: 442884
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 29 (16.3 bits)
S2: 29 (15.8 bits)