# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.0 (March 2010); http://hmmer.org/
# Copyright (C) 2010 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: P00128.hmm
# target sequence database: ITAG2.3_proteins.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: Alpha-amylase [M=316]
Accession: PF00128.19
Description: Alpha amylase, catalytic domain
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
1.8e-25 91.2 0.0 4.1e-25 90.0 0.0 1.5 1 Solyc04g078930.2.1 genomic_reference:SL2.40ch04 gene_region:
7e-24 86.0 0.7 2.9e-22 80.7 0.0 3.2 2 Solyc02g066950.2.1 genomic_reference:SL2.40ch02 gene_region:
1.1e-23 85.3 0.7 6.2e-14 53.3 0.1 3.4 3 Solyc07g014590.2.1 genomic_reference:SL2.40ch07 gene_region:
1.8e-23 84.6 0.0 5.5e-23 83.0 0.0 1.7 1 Solyc05g007070.2.1 genomic_reference:SL2.40ch05 gene_region:
4.5e-22 80.0 0.0 1.4e-21 78.4 0.0 1.7 1 Solyc03g095710.2.1 genomic_reference:SL2.40ch03 gene_region:
5.2e-19 70.0 0.5 1.2e-16 62.2 0.3 2.5 1 Solyc09g009190.2.1 genomic_reference:SL2.40ch09 gene_region:
8e-19 69.4 0.0 3.9e-18 67.1 0.0 1.9 1 Solyc04g082090.2.1 genomic_reference:SL2.40ch04 gene_region:
3.2e-17 64.1 0.0 1.8e-15 58.4 0.0 3.0 1 Solyc06g009220.2.1 genomic_reference:SL2.40ch06 gene_region:
3.2e-14 54.2 1.5 1.1e-11 45.9 1.1 2.5 1 Solyc04g082400.2.1 genomic_reference:SL2.40ch04 gene_region:
1.5e-11 45.5 0.2 5.8e-11 43.5 0.1 2.0 1 Solyc07g064830.2.1 genomic_reference:SL2.40ch07 gene_region:
6.3e-11 43.4 0.0 1.3e-08 35.8 0.0 2.9 3 Solyc09g064800.1.1 evidence_code:10F0H1E1IEG genomic_referen
6.7e-10 40.0 0.8 0.016 15.8 0.0 3.6 4 Solyc11g008050.1.1 evidence_code:10F0H1E1IEG genomic_referen
------ inclusion threshold ------
0.19 12.3 0.1 0.6 10.6 0.1 1.7 1 Solyc10g007900.2.1 genomic_reference:SL2.40ch10 gene_region:
0.36 11.3 0.2 1.7 9.2 0.1 1.9 1 Solyc07g062510.2.1 genomic_reference:SL2.40ch07 gene_region:
Domain annotation for each sequence (and alignments):
>> Solyc04g078930.2.1 genomic_reference:SL2.40ch04 gene_region:61180605-61182663 transcript_region:SL2.40ch04:61180605.
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 90.0 0.0 1.7e-28 4.1e-25 6 304 .. 27 325 .. 22 327 .. 0.73
Alignments for each domain:
== domain 1 score: 90.0 bits; conditional E-value: 1.7e-28
HGGGHHHHHH----EEEE---EEBGGTTGGG-ECEEEEE-.TT---HHHHHHHHHHHHCTT-EEEEEE-TSBEETTSCCHHHHHHHTTSC. CS
Alpha-amylase 6 iieKLdYlkdlGvtaiwlsPifespqsyhgYditdykkid.pefGtledfkeLiskahekgikvilDlVvNHtsdehewfkeslkskdnp. 94
+i+ ++l + Gvt +wl P +s s gY +++d +fG+ +++k+Li+++h++gik + D+V+NH + +++ ++ ++ +
Solyc04g078930.2.1 27 LINLVPDLAKAGVTHVWLPPSSHSV-SPQGYMPGRLYDLDaSKFGNQQQLKTLIKALHDHGIKSVADIVINHRTADNKDSRGIYSIFEGGt 116
6777899**************9988.89999998888887368*****************************9999988777777666554 PP
CGGGBSEE.--ETTSSSBB-TT--S-CCEETTTTE-SS-TTSEBB-TTSHHHHHHHHH.HHHHHHH------EETTGGGS---HHHHHHHH CS
Alpha-amylase 95 yrdyyiwkdgkkepptnwkslfggsaweddeeaqehkflkslpdlnteneevvkelkk.vvkfwldkgvDGfRlDavkhiskefwkeftqa 184
++d +w + k+ ++ s+ + + ++ +++ pd+++ n+ v+kel + + ++ + g DG+R+D v ++ k ++ +
Solyc04g078930.2.1 117 SDDRLDWGPS-----FICKNDTQYSDGTGNPDTG--LDFEPAPDIDHLNTRVQKELSDwMNWLKSEIGFDGWRFDFVRGYAPCITKIYMGN 200
8888889866.....2222234466777777774..899*******************66656666669***********99999999887 PP
HHHHTS----B-------HHHHHHHHHHTTCSCS--BSHCHHHHHHHHCS....TTSB--SSHHHHHHHHHHHHHHSTC............ CS
Alpha-amylase 185 lneykeevftvgEvfgasdedarvdatesymeleslldfedfdlaekvke....ksqlksitakklkevlsktlselse............ 259
f vgE++++ +d +++y++ + ++++ ++ + + + +++ l+++++++l +l++
Solyc04g078930.2.1 201 TS----PDFAVGELWNSLAY--GQDGKPEYNQDNHRNELVGWVKNAGRAVtafdFTTKG-----ILQAAVQEELWRLKDpngkppgmigvl 280
77....4599******6533..3333333333333444444433332221111122222.....223333333333332223344577778 PP
GGGEEB-SS-TTSHHHHHHTT-HHHHHHHHHHHHHT---EBEEE- CS
Alpha-amylase 260 deeqvtflenHDqeRvlsrfgddsaklklalallltlrGtpviYy 304
+++vtf++nHD+ ++++ s+k+ + a +lt++G+p ++y
Solyc04g078930.2.1 281 PRKAVTFIDNHDTGSTQNMWPFPSDKVMQGYAYILTHPGIPSVFY 325
89*******************9999******************99 PP
>> Solyc02g066950.2.1 genomic_reference:SL2.40ch02 gene_region:31778592-31790811 transcript_region:SL2.40ch02:31778592.
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? -0.8 0.8 0.74 1.8e+03 192 254 .. 280 342 .. 257 356 .. 0.66
2 ! 80.7 0.0 1.2e-25 2.9e-22 7 304 .. 531 820 .. 529 830 .. 0.79
Alignments for each domain:
== domain 1 score: -0.8 bits; conditional E-value: 0.74
---B-------HHHHHHHHHHTTCSCS--BSHCHHHHHHHHCSTTSB--SSHHHHHHHHHHHH CS
Alpha-amylase 192 vftvgEvfgasdedarvdatesymeleslldfedfdlaekvkeksqlksitakklkevlsktl 254
+ + +E ++s e++r+date++++ ++ +++e +++++++ ++++ ++ ++++ + + +
Solyc02g066950.2.1 280 ALQQEEEIKSSLEKMRKDATEKKKAASKAFKLELERMKTAIEAAKETARSQDEAYTRRCEALQ 342
455556666678899999999999999999999999999988644444434344444444444 PP
== domain 2 score: 80.7 bits; conditional E-value: 1.2e-25
GGGHHHHHH----EEEE---EEBGGTTGGG-ECEEEEE-TT---HHHHHHHHHHHHCTT-EEEEEE-TSBEETTSCCHHHHHHHTTSCCGG CS
Alpha-amylase 7 ieKLdYlkdlGvtaiwlsPifespqsyhgYditdykkidpefGtledfkeLiskahekgikvilDlVvNHtsdehewfkeslkskdnpyrd 97
+K +l + G+t++w+ P++es + gY +d ++++ +G le++k i+++h++++ + D+V NH ++ + ++ +
Solyc02g066950.2.1 531 ANKAADLSRCGITSVWFPPPTESV-APQGYMPSDLYNLNSAYGSLEELKGCIEEMHNQDLLALGDVVLNHRCAHKQSPNGVWNIFG----G 616
57888999****************.99*******************************************9888775444444333....2 PP
GBSEE.--ETTSSSBB-TT--S-CCEETTTTE-SS-TTSEBB-TTSHHHHHHHHH.HHHHHHH------EETTGGGS---HHHHHHHHHHH CS
Alpha-amylase 98 yyiwkdgkkepptnwkslfggsaweddeeaqehkflkslpdlnteneevvkelkk.vvkfwldkgvDGfRlDavkhiskefwkeftqalne 187
w ++ + + ++++ + + + + p +++++e v++ +kk + ++ d g DG+RlD v +s +ke+ +a+n
Solyc02g066950.2.1 617 KLAWGPE--------AIVCDDPNFQGRGNPSSGDIFHAAPNIDHSQEFVRQDIKKwLNWLRNDVGFDGWRLDFVRGFSGAYVKEYIEASN- 698
2334422........224566677766666556788999****************5555555556************************9. PP
HTS----B-------HHHHHHHHHHTTCSCS--BSHCHHHHHHHHCSTTSB--SS....HHHHHHHHHHHHHHSTC.....GGGEEB-SS- CS
Alpha-amylase 188 ykeevftvgEvfgasdedarvdatesymeleslldfedfdlaekvkeksqlksit....akklkevlsktlselse.....deeqvtflen 269
+f +gE++++ + +++++ + +++ + + +++++ +++k i +++ +++ + + + +++vtflen
Solyc02g066950.2.1 699 ---PAFSIGEYWDSLAYEGGNLCYNQDAHRQRIINWINATGGSSSAFDVTTKGILhsalHNQYWRLIDPQGKPT-GvmgwwPSRAVTFLEN 785
...69********9887777788888888888899888888888775333333332222444444444433333.246688899******* PP
TTSHHHHHHTT-HHHHHHHHHHHHHT---EBEEE- CS
Alpha-amylase 270 HDqeRvlsrfgddsaklklalallltlrGtpviYy 304
HD+ + + ++kl + a +lt++Gtpvi+y
Solyc02g066950.2.1 786 HDTGSTQGHWPFPRDKLTQGYAYILTHPGTPVIFY 820
**********************************9 PP
>> Solyc07g014590.2.1 genomic_reference:SL2.40ch07 gene_region:4925611-4940909 transcript_region:SL2.40ch07:4925611..49
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 53.3 0.1 2.5e-17 6.2e-14 1 83 [. 243 350 .. 243 377 .. 0.84
2 ! 14.5 0.0 1.6e-05 0.039 139 175 .. 383 420 .. 379 447 .. 0.80
3 ! 13.8 0.0 2.6e-05 0.065 275 313 .. 582 622 .. 526 627 .. 0.70
Alignments for each domain:
== domain 1 score: 53.3 bits; conditional E-value: 2.5e-17
TT--HHGGGHHHHHH----EEEE---EE...............BGGTTGGG-ECEEEEE-TT-..........--HHHHHHHHHHHHCTT- CS
Alpha-amylase 1 GDlkGiieKLdYlkdlGvtaiwlsPife...............spqsyhgYditdykkidpef..........GtledfkeLiskahekgi 66
G + G+ eKLd lk+lGv+ i l+P e ++ + +gY + ++ + ++ G ++fk L+++ah++gi
Solyc07g014590.2.1 243 GTYLGVVEKLDHLKELGVNCIELMPCHEfneleyysynsvlgdYKFNFWGYSTVNFFSPMGRYssaglsncglGAINEFKYLVKEAHKRGI 333
6778******************************98887766433399********998777655555555559***************** PP
EEEEEE-TSBEETTSCC CS
Alpha-amylase 67 kvilDlVvNHtsdehew 83
vi+D+V+NHt++ +e+
Solyc07g014590.2.1 334 EVIMDVVFNHTAEGNEN 350
***********998776 PP
== domain 2 score: 14.5 bits; conditional E-value: 1.6e-05
B-TTSHHHHHHHHHHHHHHHH--.----EETTGGGS-- CS
Alpha-amylase 139 lnteneevvkelkkvvkfwldkg.vDGfRlDavkhisk 175
+n +n+ v++ + + +++w+ + vDGfR+D ++ +
Solyc07g014590.2.1 383 FNCNNPIVRQFIVDCLRYWVTEMhVDGFRFDLASILTR 420
7899***************98877*******9998776 PP
== domain 3 score: 13.8 bits; conditional E-value: 2.6e-05
HHHHTT-HHH..HHHHHHHHHHT---EBEEE---TT----- CS
Alpha-amylase 275 vlsrfgddsa..klklalallltlrGtpviYyGeEigmtgg 313
++s f ++ ++++ + l+ ++G+p+iY G+E g t g
Solyc07g014590.2.1 582 FASIFVKKLRkrQMRNFFLCLMVSQGVPMIYMGDEYGHTKG 622
33333333335578889999999**************8865 PP
>> Solyc05g007070.2.1 genomic_reference:SL2.40ch05 gene_region:1652423-1664598 transcript_region:SL2.40ch05:1652423..16
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 83.0 0.0 2.2e-26 5.5e-23 7 303 .. 522 811 .. 518 814 .. 0.73
Alignments for each domain:
== domain 1 score: 83.0 bits; conditional E-value: 2.2e-26
GGGHHHHHH----EEEE---EEBGGTTGGG-ECEEEEE-TT---HHHHHHHHHHHHCTT-EEEEEE-TSBEETTSCCHHHHHHHTTSCCGG CS
Alpha-amylase 7 ieKLdYlkdlGvtaiwlsPifespqsyhgYditdykkidpefGtledfkeLiskahekgikvilDlVvNHtsdehewfkeslkskdnpyrd 97
+K l +lG+++iwl P+++s s gY +d ++++ ++G +++k ++k he gikv+ D+V NH ++++ + ++ +
Solyc05g007070.2.1 522 HDKAAELSSLGFSVIWLPPPTDSV-SPEGYMPRDLYNLNSRYGSFDELKVTVKKFHEVGIKVLGDVVLNHRCASERNQNGIWNIFG----- 606
67888899**************99.99*******************************************9888886333333222..... PP
GBSEE.--ETTSSSBB-...TT--S-CCEETTTTE-SS-TTSEBB-TTSHHHHHHHHH.HHHHHHH------EETTGGGS---HHHHHHHH CS
Alpha-amylase 98 yyiwkdgkkepptnwks...lfggsaweddeeaqehkflkslpdlnteneevvkelkk.vvkfwldkgvDGfRlDavkhiskefwkeftqa 184
g nw + + + + ++ + + +++ p +++++e v+k + + ++++ + g DG+RlD v fw + ++
Solyc05g007070.2.1 607 ------G----RLNWDEravVADDPHFQGRGNKSSGDNFHAAPNIDHSQEFVRKDIREwLLWLREEIGYDGWRLDFVRG----FWGGYVKD 683
......1....222222223556666666666656789999*******999999999955544445558******9985....66666666 PP
HHHHTS----B-------HHHHHHHHHHTTCSCS--BSHCHHHHHHHHCSTTSB--SS...HHHHHHHHHHHHHHSTC......GGGEEB- CS
Alpha-amylase 185 lneykeevftvgEvfgasdedarvdatesymeleslldfedfdlaekvkeksqlksit...akklkevlsktlselse......deeqvtf 266
e++e f vgE++++ + ++ ++++ + ++d + + +++ + +++k i +++++ + ++++ + +++vtf
Solyc05g007070.2.1 684 YLEATEPYFAVGEFWDSLVYTYGEMDHNQDAHRQRIIDWINATNGTAGAFDVTTKGILhsaIERCEYWRLSDQKGKPPgvvgwwPSRAVTF 774
666666789999999977665555555556666677777666655555532222222133355566555555555555687778889**** PP
SS-TTSHHHHHHTT-HHHHHHHHHHHHHT---EBEEE CS
Alpha-amylase 267 lenHDqeRvlsrfgddsaklklalallltlrGtpviY 303
+enHD+ + + k + a +lt++Gtp ++
Solyc05g007070.2.1 775 IENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPSVF 811
********9888888777899*************987 PP
>> Solyc03g095710.2.1 genomic_reference:SL2.40ch03 gene_region:50338173-50341110 transcript_region:SL2.40ch03:50338173.
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 78.4 0.0 5.7e-25 1.4e-21 6 304 .. 52 350 .. 47 352 .. 0.72
Alignments for each domain:
== domain 1 score: 78.4 bits; conditional E-value: 5.7e-25
HGGGHHHHHH----EEEE---EEBGGTTGGG-ECEEEEE-.TT---HHHHHHHHHHHHCTT-EEEEEE-TSBEETTSCC.HHHHHHHTTSC CS
Alpha-amylase 6 iieKLdYlkdlGvtaiwlsPifespqsyhgYditdykkid.pefGtledfkeLiskahekgikvilDlVvNHtsdehew.fkeslkskdnp 94
+i+ ++l Gvt +wl P +s s gY +++d +fG+ +++k+Li+++ ++gik + D+V+NH +++ + ++ +
Solyc03g095710.2.1 52 LINLVPDLAHAGVTHVWLPPSSHSV-SPQGYMPGRLYDLDaSKFGNQQELKALIKALRDYGIKSVADIVINHRCADKKDsRGIYCIFEGGT 141
67778999*************9988.89999998888887368*****************************7666654144555555666 PP
CGGGBSEE.--ETTSSSBB-TT--S-CCEETTTTE-SS-TTSEBB-TTSHHHHHHHHH.HHHHHHH------EETTGGGS---HHHHHHHH CS
Alpha-amylase 95 yrdyyiwkdgkkepptnwkslfggsaweddeeaqehkflkslpdlnteneevvkelkk.vvkfwldkgvDGfRlDavkhiskefwkeftqa 184
+d +w + k+ + s+ + + ++ +++ pd+++ n v+kel + + ++ + g DG+R+D v ++ + k ++ +
Solyc03g095710.2.1 142 TDDRLDWGPS-----FICKDDIQYSDGTGNLDT--GLDFEPAPDIDHLNIRVQKELSDwMNWLKSEIGFDGWRFDFVRGYAPNITKIYMGN 225
8888889855.....222222335555555555..589********************66656666669************9999999988 PP
HHHHTS----B-------..HHHHHHHHHHTTCSCS--BSHCHHHHHH.HHCSTTSB--SS.HHHHHHHHHHHHHHSTC......GGGEEB CS
Alpha-amylase 185 lneykeevftvgEvfgas..dedarvdatesymeleslldfedfdlae.kvkeksqlksit.akklkevlsktlselse......deeqvt 265
f vgE++++ ++d + d ++ ++ +e l+ +++ + +++++ +++ + a + + + k+ + + +++vt
Solyc03g095710.2.1 226 TS----PDFAVGEFWNSLayGQDGKPDYNQDNHRNE-LVGWVQNGGGAvTAFDFTTKGILQaAVEGELWRLKDSHGKPPgmigvlPQNAVT 311
87....4599*****976443333444444444444.33333333222022222222222223333333222222222246666899**** PP
-SS-TTSHHHHHHTT-HHHHHHHHHHHHHT---EBEEE- CS
Alpha-amylase 266 flenHDqeRvlsrfgddsaklklalallltlrGtpviYy 304
f++nHD+ ++++ s+k+ + a +lt++G+p+++y
Solyc03g095710.2.1 312 FIDNHDTGSTQNMWPFPSDKVMQGYAYILTHPGIPLVFY 350
***************9999******************99 PP
>> Solyc09g009190.2.1 genomic_reference:SL2.40ch09 gene_region:2539818-2558047 transcript_region:SL2.40ch09:2539818..25
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 62.2 0.3 5e-20 1.2e-16 10 175 .. 370 510 .. 364 566 .. 0.77
Alignments for each domain:
== domain 1 score: 62.2 bits; conditional E-value: 5e-20
HHHHHH----EEEE---EEBGG.TTGGG-ECEEEEE-TT---HHHHHHHHHHHHCTT-EEEEEE-TSBEETTSCCHHHHHHHTTSCCGGGB CS
Alpha-amylase 10 LdYlkdlGvtaiwlsPifespq.syhgYditdykkidpefGtledfkeLiskahekgikvilDlVvNHtsdehewfkeslkskdnpyrdyy 99
L+ +k+lG++a++++ i e ++ + +gY++t++ + +fGt +d+k+Li+kahe gi v++D+V H s++ l+ d + +y
Solyc09g009190.2.1 370 LPRIKKLGYNAVQIMAIQEHSYyASFGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGIVVLMDIVHSHASNNTLD---GLNMFDG-TDSCY 456
7789***************5555*********************************************9998874...4443442.33333 PP
SEE.--ETTSSSBB-TT--S-CCEETTTTE-SS-TTSEBB-TTSHHHHHHHHHHHHHHHH--.----EETTGGGS-- CS
Alpha-amylase 100 iwkdgkkepptnwkslfggsaweddeeaqehkflkslpdlnteneevvkelkkvvkfwldkg.vDGfRlDavkhisk 175
+ + g +++ +++ +n+ n ev ++l++ ++ wld+ DGfR+D v +
Solyc09g009190.2.1 457 F--------------HSGARGYHWMWDSR---------LFNYGNWEVLRYLLSNARWWLDEFkFDGFRFDGVTSMMY 510
3..............23334455555552.........38999999*************9999*******9887654 PP
>> Solyc04g082090.2.1 genomic_reference:SL2.40ch04 gene_region:63474186-63478355 transcript_region:SL2.40ch04:63474186.
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 67.1 0.0 1.6e-21 3.9e-18 5 304 .. 38 327 .. 35 329 .. 0.76
Alignments for each domain:
== domain 1 score: 67.1 bits; conditional E-value: 1.6e-21
HHGGGHHHHHH----EEEE---EEBGGTTGGG-ECEEEEE-TT---HHHHHHHHHHHHCTT-EEEEEE-TSBEETTSCCHHHHHHHTTSCC CS
Alpha-amylase 5 GiieKLdYlkdlGvtaiwlsPifespqsyhgYditdykkidpefGtledfkeLiskahekgikvilDlVvNHtsdehewfkeslkskdnpy 95
+ K +++ + G+t+ wl P+ +s + gY ++ ++++ +G ++ +++L++k++++ ++ + D+V+NH + ++ +d
Solyc04g082090.2.1 38 NLDMKVPDIAKSGITTAWLPPVCHSL-APEGYLPQNLYSLNSSYGSEDLLNALLDKLKQYKVRGMADIVINHRVGTTQGHGGIYNRYD--- 124
567899******************99.99*******************************************8877776222222222... PP
GGGBSEE.--ETTSSSBB-TT--S..-CCEETTTTE-SS-TTSEBB-TTSHHHHHHHHHHHHHHHH--..----EETTGGGS---HHHHHH CS
Alpha-amylase 96 rdyyiwkdgkkepptnwkslfggs..aweddeeaqehkflkslpdlnteneevvkelkkvvkfwldkg..vDGfRlDavkhiskefwkeft 182
p w ++ s + ++ + +++++ p +++++ v+k l + ++ wl+ + fR+D +k ++++ +ke+
Solyc04g082090.2.1 125 -G----------IPMSWDEHAITSctGGRGNKST--GDNFNGFPNIDHTQSFVRKDLIDWMR-WLRSSvgFQDFRFDFAKGYASKYVKEYI 201
.2..........2333433222220022223333..469********************664.6665534899***************999 PP
HHHHHHTS----B-------HHHHHHHHHHTTCSCS--BSHCHHHHHHHHCSTTSB--SSHHHHHHHHHHHHHHSTC........GGGEEB CS
Alpha-amylase 183 qalneykeevftvgEvfgasdedarvdatesymeleslldfedfdlaekvkeksqlksitakklkevlsktlselse........deeqvt 265
+ + +f vgE++++ + + ++++ + +++ d + +++ +++k i +++k + + +++ + +++vt
Solyc04g082090.2.1 202 EGAK----PIFAVGEYWDTCNYKGSNLDYNQDSHRQRIINWIDGAGQLSTAFDFTTKAILQEAVKGEFWRLRDSKGKppgvlgwwPSRAVT 288
9988....79********9988888888888888887888777665555554344444443333333333333222233588888899*** PP
-SS-TTSHHHHHHTT-HHHHHHHHHHHHHT---EBEEE- CS
Alpha-amylase 266 flenHDqeRvlsrfgddsaklklalallltlrGtpviYy 304
f++nHD+ + + s+++ a +lt++G+p ++y
Solyc04g082090.2.1 289 FIDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGIPSVFY 327
**************99999******************99 PP
>> Solyc06g009220.2.1 genomic_reference:SL2.40ch06 gene_region:3155576-3182364 transcript_region:SL2.40ch06:3155576..31
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 58.4 0.0 7.1e-19 1.8e-15 1 311 [. 257 615 .. 257 621 .. 0.69
Alignments for each domain:
== domain 1 score: 58.4 bits; conditional E-value: 7.1e-19
TT--HHGGGHHHHHH----EEEE---EE.............BGG.TTGGG-ECEEEEE-TT-.....--...HHHHHHHHHHHHCTT-EEE CS
Alpha-amylase 1 GDlkGiieKLdYlkdlGvtaiwlsPife.............spq.syhgYditdykkidpef.....Gt...ledfkeLiskahekgikvi 69
G + G+ieK++ l +lGv+++ l P+fe ++ + +gY + ++ + ++ G ++ke+++++h gi vi
Solyc06g009220.2.1 257 GSYLGLIEKIPHLLELGVNTVELLPVFEfdelelqrrpnprDHMiNTWGYSTINFFAPMSRYascggGPvraSWELKEMVKALHGAGIEVI 347
7788********************************8877633479****************6664222222458**************** PP
EEE-TSBEETTSCCHHHHHHHTTSCCGGGBSEE.--ETTSSSBB-TT--S-CCEETTTTE-SS-TTSEBB-TTSHHHHHHHHHHHHHHHH- CS
Alpha-amylase 70 lDlVvNHtsdehewfkeslkskdnpyrdyyiwkdgkkepptnwkslfggsaweddeeaqehkflkslpdlnteneevvkelkkvvkfwldk 160
lD+V+NHt+++++ +++ +s + +++ y + ++ n++ + +++ + ++ +n + ++v++ +++ +++w+ +
Solyc06g009220.2.1 348 LDVVYNHTNEADDE-NPYTTSFRGIDNKVYYM------VDLNNN--AQLLNFSGCGNT-----------FNCNHPTVMELILESLRHWVTE 418
**********9996.88888777665555542......222222..234556655555...........99************99999976 PP
-.----EETTGGGS--..................................................-HHHHHHHHHHHHT.S----B---- CS
Alpha-amylase 161 g.vDGfRlDavkhisk..................................................efwkeftqalneyk.eevftvgEvf 199
vDGfR+D ++ + e+ ++++++ + ++ + g +
Solyc06g009220.2.1 419 YhVDGFRFDLASVLCRgtdgtpinapplvkaisrdsvlsrckiiaepwdcgglylvgkfpnwdrwaEWNGKYRDDIRRFIkGDAGMKGNFA 509
66************999999*9999999999999999999999999999999999999977733333333333333333333333333333 PP
---HHHHHHHHHHTTCSCS--BSHCHHHHHHHHCSTTSB--SSHHHHHHHHHHHHHHSTC...GGGEEB-SS-TTSHHHHHHTT-HHH... CS
Alpha-amylase 200 gasdedarvdatesymeleslldfedfdlaekvkeksqlksitakklkevlsktlselse...deeqvtflenHDqeRvlsrfgddsa... 284
a+ + + + ++v+ + +++ +t+++l ++ +k ++ e d + +f n e s+++ ++
Solyc06g009220.2.1 510 TRIAGSADL------------YRVNKRKPYHSVNFVIAHDGFTLYDLVSYNNKHNDANGEggnDGCNDNFSWNCGIEGETSDANVNELrsr 588
333333333............3333444444444444444455555555555444444443333334444444444443333333333444 PP
HHHHHHHHHHT---EBEEE---TT--- CS
Alpha-amylase 285 klklalallltlrGtpviYyGeEigmt 311
++k+ +l+ ++Gtp++ G+E g t
Solyc06g009220.2.1 589 QMKNFHLALMVSQGTPMMLMGDEYGHT 615
789999999999999999999999876 PP
>> Solyc04g082400.2.1 genomic_reference:SL2.40ch04 gene_region:63662213-63669892 transcript_region:SL2.40ch04:63662213.
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 45.9 1.1 4.3e-15 1.1e-11 10 197 .. 293 484 .. 287 579 .. 0.63
Alignments for each domain:
== domain 1 score: 45.9 bits; conditional E-value: 4.3e-15
HHHHHH----EEEE---EEBGG.TTGGG-ECEEEEE-TT---HHHHHHHHHHHHCTT-EEEEEE-TSBEETTSCCHHHHHHHTTSCCGGGB CS
Alpha-amylase 10 LdYlkdlGvtaiwlsPifespq.syhgYditdykkidpefGtledfkeLiskahekgikvilDlVvNHtsdehewfkeslkskdnpyrdyy 99
L+ +k ++++++l+ i+e ++ + +gY++t++ ++ + G+ ed+k Li+kah+ g++v++D+V H s++ l+ +
Solyc04g082400.2.1 293 LPRIKANNYNTVQLMAIMEHSYyGSFGYHVTNFFAVSSRSGNPEDLKYLIDKAHSLGLQVLVDVVHSHASNNVTD---GLN--------GF 372
6668888999*********5555**********************************************999884...333........22 PP
SEE.--ETTSSSBB-TT--S-CCEETTTTE-SS-TTSEBB-TTSHHHHHHHHHHHHHHHH--.----EETTGGGS--..........-HHH CS
Alpha-amylase 100 iwkdgkkepptnwkslfggsaweddeeaqehkflkslpdlnteneevvkelkkvvkfwldkg.vDGfRlDavkhisk..........efwk 179
++ +g ++ +s ++ e + +++l++ +n+ n ev + l++ ++ wl++ DGfR+D + + +
Solyc04g082400.2.1 373 DIGQG--SQ---------ESYFHAGERG--YHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYnFDGFRFDGITSMLYvhhginmgftG--- 447
32222..11.........2233333333..4455555678899999999999999999987679999999887765556666664442... PP
HHHHHHHHHT...................S----B-- CS
Alpha-amylase 180 eftqalneyk...................eevftvgE 197
++++ e++ +++ +++E
Solyc04g082400.2.1 448 NYNEYFSEATdvdavvylmlannlihkifPDATVIAE 484
2222222222222444444444444444555555555 PP
>> Solyc07g064830.2.1 genomic_reference:SL2.40ch07 gene_region:64039012-64053950 transcript_region:SL2.40ch07:64039012.
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 43.5 0.1 2.3e-14 5.8e-11 5 75 .. 424 495 .. 422 619 .. 0.87
Alignments for each domain:
== domain 1 score: 43.5 bits; conditional E-value: 2.3e-14
HHGGGHHHHHH----EEEE---EEBGG.TTGGG-ECEEEEE-TT---HHHHHHHHHHHHCTT-EEEEEE-TS CS
Alpha-amylase 5 GiieKLdYlkdlGvtaiwlsPifespq.syhgYditdykkidpefGtledfkeLiskahekgikvilDlVvN 75
+i + L+ +k+ G++ai++ ++e ++ gY +t+++++ ++Gt +dfk+L+++ah g+ v+l +V
Solyc07g064830.2.1 424 FISKVLPHVKEAGYNAIQIIGVVEHKDyFTVGYRVTNFYAVSSRYGTPDDFKRLVDEAHGLGLLVFLEIVHS 495
6778899*****************7755788************************************99954 PP
>> Solyc09g064800.1.1 evidence_code:10F0H1E1IEG genomic_reference:SL2.40ch09 gene_region:57806545-57809145 transcript_r
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 35.8 0.0 5.2e-12 1.3e-08 1 82 [. 378 466 .. 378 474 .. 0.88
2 ? 3.4 0.0 0.038 94 119 165 .. 471 516 .. 466 526 .. 0.85
3 ? 0.2 0.0 0.37 9.1e+02 285 314 .. 669 698 .. 651 700 .. 0.81
Alignments for each domain:
== domain 1 score: 35.8 bits; conditional E-value: 5.2e-12
TT--HHGGGHHHHHH----EEEE---EEBGGTTGGG-ECEEEEE-TT---HHH.......HHHHHHHHHCTT-EEEEEE-TSBEETTSC CS
Alpha-amylase 1 GDlkGiieKLdYlkdlGvtaiwlsPifespqsyhgYditdykkidpefGtled.......fkeLiskahekgikvilDlVvNHtsdehe 82
G ++Gi eK lkdlGv+a l Pif +++ Y + + +++G d +k +++k+h++gi v+l +V+ Ht+++ +
Solyc09g064800.1.1 378 GTFSGISEKWHHLKDLGVNAMLLEPIFPFDEQKGPYYPWHFFSPGNMYGPSGDplsviksMKDMVKKLHANGIEVFLEVVFTHTAEDAP 466
7899************************9989999**************865444444449************************9876 PP
== domain 2 score: 3.4 bits; conditional E-value: 0.038
S-CCEETTTTE-SS-TTSEBB-TTSHHHHHHHHHHHHHHHH--.---- CS
Alpha-amylase 119 saweddeeaqehkflkslpdlnteneevvkelkkvvkfwldkg.vDGf 165
+++++ ++ ++++ + ln + + v++ +++ +++w+ + +DGf
Solyc09g064800.1.1 471 DNFSYCIKGS--QDMNIQNALNCNYPIVQQMILDCLRHWVIEFhIDGF 516
5677778885..69999*****************9999998878***9 PP
== domain 3 score: 0.2 bits; conditional E-value: 0.37
HHHHHHHHHHT---EBEEE---TT------ CS
Alpha-amylase 285 klklalallltlrGtpviYyGeEigmtggk 314
++++ l +l+ + G+pv G+E g++ g
Solyc09g064800.1.1 669 QVRNFLFILFISLGVPVLNMGDECGQSSGG 698
6788889999999************98765 PP
>> Solyc11g008050.1.1 evidence_code:10F0H1E1IEG genomic_reference:SL2.40ch11 gene_region:2256207-2264573 transcript_reg
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 6.4 0.0 0.0046 11 8 63 .. 79 137 .. 74 139 .. 0.84
2 ! 13.6 0.0 2.9e-05 0.073 53 82 .. 169 198 .. 167 236 .. 0.79
3 ! 15.8 0.0 6.3e-06 0.016 151 200 .. 243 301 .. 222 326 .. 0.74
4 ? -1.3 0.0 1 2.5e+03 219 274 .. 374 433 .. 349 442 .. 0.61
Alignments for each domain:
== domain 1 score: 6.4 bits; conditional E-value: 0.0046
GGHHHHHH----EEEE---EE...BGGTTGGG-ECEEEEE-TT---HHHHHHHHHHHHC CS
Alpha-amylase 8 eKLdYlkdlGvtaiwlsPife...spqsyhgYditdykkidpefGtledfkeLiskahe 63
++L+ l + G+t + l P ++ +++h+ td++k++ + e+ ++Li+++++
Solyc11g008050.1.1 79 QHLKRLSNAGITHVHLLPTYQfagVEDEKHKWKYTDIEKLNSFPPDSEEQQALITAIQD 137
789999***************76533399***********9988888888888887765 PP
== domain 2 score: 13.6 bits; conditional E-value: 2.9e-05
HHHHHHHHHHCTT-EEEEEE-TSBEETTSC CS
Alpha-amylase 53 dfkeLiskahekgikvilDlVvNHtsdehe 82
+f++++++++ g++v+lD+V+NH + +
Solyc11g008050.1.1 169 EFRKMVQALNRIGLRVVLDVVYNHLHASGP 198
799*********************987766 PP
== domain 3 score: 15.8 bits; conditional E-value: 6.3e-06
HHHHHHHHH--.----EETTGGGS--.......-HHHHHHHHHHHHT.S----B----- CS
Alpha-amylase 151 kkvvkfwldkg.vDGfRlDavkhisk.......efwkeftqalneyk.eevftvgEvfg 200
++ +k w+ + +DGfR+D + hi k +f++++ ++ n + ++++gE ++
Solyc11g008050.1.1 243 LDDLKCWAVHYkIDGFRFDLMGHIMKrtmlkakSFLNSLSTEENGVDgSSIYIYGEGWD 301
445666765545**************88866666666666666666599*******998 PP
== domain 4 score: -1.3 bits; conditional E-value: 1
--BSHCHHHHHHHHCSTTSB--SS..HHHHHHHHHHHHHHSTC...GGGEEB-SS-TTSHH CS
Alpha-amylase 219 slldfedfdlaekvkeksqlksit..akklkevlsktlselse...deeqvtflenHDqeR 274
d ++++a ++k+ + l++++ k +evl+++++ + + e+v+++ HD+e
Solyc11g008050.1.1 374 VSKDHIQVGMAANLKD-FVLTNCDgqEVKGSEVLMHDRKPVGYassPVETVNYVSAHDNET 433
4455555555556654.22333332245556777777776555445789999999999975 PP
>> Solyc10g007900.2.1 genomic_reference:SL2.40ch10 gene_region:2091468-2095251 transcript_region:SL2.40ch10:2091468..20
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 10.6 0.1 0.00024 0.6 47 176 .. 55 228 .. 50 277 .. 0.66
Alignments for each domain:
== domain 1 score: 10.6 bits; conditional E-value: 0.00024
T---HHHHHHHHHHHHCTT-EEEEEE-TSBEETTSCCHHHHHHHTTSCCGGGBSE.E.-.....................-ETTSSSBB-. CS
Alpha-amylase 47 efGtledfkeLiskahekgikvilDlVvNHtsdehewfkeslkskdnpyrdyyiw.kdg.....................kkepptnwks. 114
+G+++++k++i++a +k i++ Dl+ ++ +++++ +++++w ++ +k+ + + +
Solyc10g007900.2.1 55 LYGDMKEMKKMIEEATSKPINLSHDLIWPRINPFIHK---TITNYG---KNCFVWiGPKpavlitepklirevltknyvyQKARGSPLSKl 139
59*************************9888777664...333333...455555222245555555566655555444422222222223 PP
.TT--S-CCEETTTTE...-SS-TTSEBB-TTSHHHHHHHHHHHHHHHH--.----EETTGGGS--......................- CS
Alpha-amylase 115 .lfggsaweddeeaqe...hkflkslpdlnteneevvkelkkvvkfwldkg.vDGfRlDavkhisk......................e 176
+ g +a e+d++a++ + +++l++l++ +++ ++++++ w + DG +D+ ++++ e
Solyc10g007900.2.1 140 vISGLAAHEKDKWATHrriLNPAFHLDKLKHMLPAFKLTINEMLNTWKEVVsKDGTEIDVWPYLQTltsdaisrtafgsnyeegkkifE 228
35566666666666666777888999999999999888888888888886668999999998888866666666666666655555552 PP
>> Solyc07g062510.2.1 genomic_reference:SL2.40ch07 gene_region:62439678-62443172 transcript_region:SL2.40ch07:62439678.
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 9.2 0.1 0.00067 1.7 48 74 .. 51 77 .. 45 275 .. 0.63
Alignments for each domain:
== domain 1 score: 9.2 bits; conditional E-value: 0.00067
---HHHHHHHHHHHHCTT-EEEEEE-T CS
Alpha-amylase 48 fGtledfkeLiskahekgikvilDlVv 74
+G+++df ++i++a++k +++ D+ +
Solyc07g062510.2.1 51 YGDMKDFSKMIKEANSKPMNLSNDITP 77
9*******************9988765 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (316 nodes)
Target sequences: 34727 (11990819 residues)
Passed MSV filter: 1793 (0.0516313); expected 694.5 (0.02)
Passed bias filter: 932 (0.0268379); expected 694.5 (0.02)
Passed Vit filter: 90 (0.00259164); expected 34.7 (0.001)
Passed Fwd filter: 14 (0.000403145); expected 0.3 (1e-05)
Initial search space (Z): 34727 [actual number of targets]
Domain search space (domZ): 14 [number of targets reported over threshold]
# CPU time: 1.27u 0.06s 00:00:01.33 Elapsed: 00:00:00.34
# Mc/sec: 11144.41
//