LOCUS AB000001 660 bp DNA linear PLN 12-SEP-2002
DEFINITION Rhizoctonia solani genes for 18S rRNA, 5.8S rRNA, 28S rRNA, partial
and complete sequence, isolate:#1.
ACCESSION AB000001
VERSION AB000001.1 GI:1754539
PROJECT GenomeProject:18357
KEYWORDS .
SOURCE Rhizoctonia solani
ORGANISM Rhizoctonia solani
Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
Agaricomycetes; Cantharellales; Ceratobasidiaceae; Thanatephorus.
REFERENCE 1
AUTHORS Kuninaga,S., Natsuaki,T., Takeuchi,T. and Yokosawa,R.
TITLE Sequence variation of the rDNA ITS regions within and between
anastomosis groups in Rhizoctonia solani
JOURNAL Curr. Genet. 32 (3), 237-243 (1997)
PUBMED 9339350
REFERENCE 2 (bases 1 to 660)
AUTHORS Kuninaga,S.
TITLE Direct Submission
JOURNAL Submitted (19-DEC-1996) Shiro Kuninaga, Health Sciences University
of Hokkaido, General Education; 1757 Kanazawa, Tohbetsu, Hokkaido
061-02, Japan (E-mail:kuninaga@hoku-iryo-u.ac.jp,
Tel:81-1332-3-1211, Fax:81-1332-3-1276)
FEATURES Location/Qualifiers
source 1..660
/organism="Rhizoctonia solani"
/mol_type="genomic DNA"
/strain="AG-3"
/isolate="#1"
/db_xref="taxon:456999"
/dev_stage="22 day-old seedlings (14 days after
inoculation with Bradyrhizobium japonicum strain USDA110)"
/clone_lib="Soybean Shoot cDNA Library"
/note="Organ: Autoregulated shoots; Vector: lambda ZAPII;
Double stranded cDNAs were synthesized by the method of
Gubler and Hoffman (1983), using a cDNA synthesis kit
(Amersham Life Science Inc.) and then ligated to an EcoRI
adaptor (Amersham). cDNA library was constructed in
lambda ZAPII vector using lambda ZAPII/EcoRI/Gigapack II
cloning kit (Stratagene)"
rRNA <1..6
/product="18S ribosomal RNA"
rRNA 229..383
/product="5.8S ribosomal RNA"
rRNA 656..>660
/product="28S ribosomal RNA"
CDS join(1677..1980,2158..2630,2729..3580)
/gene="PHT5"
/codon_start=1
/product="inorganic phosphate transporter"
/protein_id="BAA24280.1"
/db_xref="GI:2780345"
/translation="MAKKGKEVLNALDAAKTQMYHFTAIVIAGMGFFTDAYDLFSISL
VTKLLGRIYYHVDSSKKPGTLPPNVAAAVNGVAFCGTLAGQLFFGWLGDKLGRKKVYG
ITLMLMVLCSLGSGLSFGHSANGVMATLCFFRFWLGFGIGGDYPLSATIMSEYANKKT
RGAFIAAVFAMQGFGILAGGIVSLIVSSTFDHAFKAPTYEVDPVGSTVPQADYVWRIV
LMFGAIPALLTYYWRMKMPETARYTALVARNTKQAASDMSKVLQVDLIAEEEAQSNSN
SSNPNFTFGLFTREFARRHGLHLLGTTTTWFLLDIAYYSSNLFQKDIYTAIGWIPAAE
TMNAIHEVFTVSKAQTLIALCGTVPGYWFTVAFIDILGRFFIQLMGFIFMTIFMFALA
IPYDHWRHRENRIGFLIMYSLTMFFANFGPNATTFVVPAEIFPARLRSTCHGISAASG
KAGAIVGAFGFLYAAQSSDSEKTDAGYPPGIGVRNSLLMLACVNFLGIVFTLLVPESK
GKSLEEISREDEEQSGGDTVVEMTVANSGRKVPV"
BASE COUNT 163 a 172 c 196 g 188 t 10 others
ORIGIN
1 aattttaatg aagagtttgg ttgtagctgg cccattaatt taggcatgtg cacacctttc
61 tctttcatcc catacacacc tgtgaacttg tgagacagat ggggaattta tttattgttt
121 ttttttgtaa tataaagatg ataagtcatt gaacccttct gtctactcaa ctcatataaa
181 ctcaatttat tttaaaatga atgtaatgga tgtaacgcat ctaatactaa gtttcaacaa
241 cggatctctt ggctctcgca tcgatgaaga acgcagcgaa atgcgataag taatgtgaat
301 tgcagaattc agtgaatcat cgaatctttg aacgcacctt gcgctccttg gtattccttg
361 gagcatgcct gtttgagtat catgaaatct tcaaaatcaa gtcttttgtt aattcaattg
421 gctttgactt tggtattgga ggtctttgca gcttcacacc tgctcctctt tgtacattag
481 ctggatctca gtgttatgct tggttccact cagcgtgata agttatctat cgctgaggac
541 actgtaaaaa ggtggccaag gtaaatgcag atgaaccgct tctaatagtc cattgacttg
601 gacaatattt ttatgatctg atctcaaatc aggtaggact acccgctgaa cttaagcata
//
LOCUS YSCPLASM 6318 bp DNA circular PLN 18-OCT-2007
DEFINITION Saccharomyces cerevisiae 2 micron circle plasmid, complete
sequence.
ACCESSION J01347 L00321 L00322 L00323 L00324 M10185 M11111 M11593
M14239-M14245 M14253-M14259 M14591-M14598 V01323
VERSION J01347.1 GI:172190
KEYWORDS DNA-binding protein; Rep-1 protein; Rep-2 protein; circular;
complete genome; d protein; plasmid; protein FLP; recombinase;
repeat region.
SOURCE Saccharomyces cerevisiae (baker's yeast)
ORGANISM Saccharomyces cerevisiae
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomyceta;
Saccharomycotina; Saccharomycetes; Saccharomycetales;
Saccharomycetaceae; Saccharomyces.
REFERENCE 1 (bases 1 to 1022)
AUTHORS Hindley,J. and Phear,G.A.
TITLE Sequence of 1019 nucleotides encompassing one of the inverted
repeats from the yeast 2 micrometer plasmid
JOURNAL Nucleic Acids Res. 7 (2), 361-375 (1979)
PUBMED 386282
REFERENCE 2 (bases 1 to 6318)
AUTHORS Hartley,J.L. and Donelson,J.E.
TITLE Nucleotide sequence of the yeast plasmid
JOURNAL Nature 286 (5776), 860-865 (1980)
PUBMED 6251374
REFERENCE 3 (bases 3891 to 3990)
AUTHORS Broach,J.R., Guarascio,V.R. and Jayaram,M.
TITLE Recombination within the yeast plasmid 2mu circle is site-specific
JOURNAL Cell 29 (1), 227-234 (1982)
PUBMED 6286142
REFERENCE 4 (bases 3881 to 4020)
AUTHORS McLeod,M., Volkert,F. and Broach,J.
TITLE Components of the site-specific recombination system encoded by the
yeast plasmid 2-micron circle
JOURNAL Cold Spring Harb. Symp. Quant. Biol. 49, 779-787 (1984)
PUBMED 6397322
REFERENCE 5 (bases 670 to 732)
AUTHORS Andrews,B.J., Proteau,G.A., Beatty,L.G. and Sadowski,P.D.
TITLE The FLP recombinase of the 2 micron circle DNA of yeast:
interaction with its target sequences
JOURNAL Cell 40 (4), 795-803 (1985)
PUBMED 3879971
REFERENCE 6 (bases 5570 to 5605)
AUTHORS Babineau,D., Vetter,D., Andrews,B.J., Gronostajski,R.M.,
Proteau,G.A., Beatty,L.G. and Sadowski,P.D.
TITLE The FLP protein of the 2-micron plasmid of yeast. Purification of
the protein from Escherichia coli cells expressing the cloned FLP
gene
JOURNAL J. Biol. Chem. 260 (22), 12313-12319 (1985)
PUBMED 2995370
REFERENCE 7 (sites)
AUTHORS Gronostajski,R.M. and Sadowski,P.D.
TITLE Determination of DNA sequences essential for FLP-mediated
recombination by a novel method
JOURNAL J. Biol. Chem. 260 (22), 12320-12327 (1985)
PUBMED 2995371
REFERENCE 8 (sites)
AUTHORS Sutton,A. and Broach,J.R.
TITLE Signals for transcription initiation and termination in the
Saccharomyces cerevisiae plasmid 2 micron circle
JOURNAL Mol. Cell. Biol. 5 (10), 2770-2780 (1985)
PUBMED 3915534
REFERENCE 9 (sites)
AUTHORS Gronostajski,R.M. and Sadowski,P.D.
TITLE The FLP recombinase of the Saccharomyces cerevisiae 2 microns
plasmid attaches covalently to DNA via a phosphotyrosyl linkage
JOURNAL Mol. Cell. Biol. 5 (11), 3274-3279 (1985)
PUBMED 2427927
REFERENCE 10 (bases 667 to 739)
AUTHORS Senecoff,J.F., Bruckner,R.C. and Cox,M.M.
TITLE The FLP recombinase of the yeast 2-micron plasmid: characterization
of its recombination site
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82 (21), 7270-7274 (1985)
PUBMED 2997780
REFERENCE 11 (sites)
AUTHORS McLeod,M., Craft,S. and Broach,J.R.
TITLE Identification of the crossover site during FLP-mediated
recombination in the Saccharomyces cerevisiae plasmid 2 microns
circle
JOURNAL Mol. Cell. Biol. 6 (10), 3357-3367 (1986)
PUBMED 3540590
COMMENT On Feb 10, 2004 this sequence version replaced gi:4182.
[8] sites; mRNA CAP sites and poly-adenylation sites. [9] sites;
FLP binding.
[7] sites; FLP cleavage.
[11] sites; FLP-mediated recombination crossover site. Draft entry
and clean copy sequence for [5] kindly provided by J.Senecoff,
24-JAN-1986.
Yeast 2 micron plasmid contains two 599 bp inverted repeats
separated by a large unique (UL) and a small unique (US) region.
During recombination the UL and US regions invert producing two
sequence forms that differ in the orientation of one unique region
relative to the other. The A form is presented below. FLP is the
only 2-micron circle-encoded protein needed for specific site
recombination between the IRs of 2-micron circle. The minimal size
of the recombination site required for efficient FLP
recombinase-catalyzed recombination in vitro is no more than 28 bp,
which includes parts of two 13 bp inverted repeats (positions
690-702 and 711-723) and all of an 8 bp spacer (703-710) [5]. The
FLP recombinase cleaves the DNA at the boundaries of the spacer and
becomes covalently linked to the spacer DNA [5],[9]. The
efficiency of the recombination is reduced if the spacer in a
recombinant site is increased or decreased by 1 bp, while the
spacer in the second site is unaltered [5]. Recombination between
two sites with identical 1-base pair additions or deletions is
relatively unaffected, suggesting that pairing of sequences in the
spacer regions is important in FLP-promoted recombination events
[5]. The sequence asymmetry utilized by the recombinase to
determine the orientation of the site is located uniquely within
the spacer region. Another 13 bp direct repeat, is found at
positions 676-688 [5]. FLP-mediated recombination involving two
FLP sites that are inverted with respect to each other results in
inversion of the DNA sequences between the sites [4]. If the
participating recombination sites are in direct orientation, FLP
promotes only the excision of the intervening DNA sequences [4].
The Rep 1 and Rep proteins are involved plasmid partitioning and
protein stability.
A start codon in phase with the Rep1 coding region is located at
positions 1966-1964. Two CAP sites for Rep1 mRNA are located
beyond the 'atg' codon (position 2008) at positions 2004 and 2005.
Complete source information:
Yeast (S.cerevisiae, strain A364A D5) DNA, clones pJDB71 [1],
p82-6B [2], CV20 [3], pMMD2 [4], pGP20 [5], pJFS166 [10].
FEATURES Location/Qualifiers
source 1..6318
/organism="Saccharomyces cerevisiae"
/mol_type="genomic DNA"
/strain="A364A"
/db_xref="taxon:4932"
/clone="pJDB71, p82-6B, CV20, pMMD2, pGP20, pJFS166"
/plasmid="2 micron circle plasmid"
mRNA join(5549..6318,1..545)
/product="Flp mRNA"
CDS join(5570..6318,1..523)
/codon_start=1
/product="recombinase (FLP)"
/protein_id="AAB59340.1"
/db_xref="GI:172191"
/translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWM
ITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEF
TIIPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEK
ILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCL
VTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLK
DNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKR
ASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKG
SAEGSIRYPAWNGIISQEVLDYLSSYINRRI"
exon 1..545
conflict 157..160
/citation=[1]
/replace=""
conflict 289..290
/citation=[1]
/replace=""
repeat_region 341..939
/note="IR2"
conflict 464..466
/citation=[1]
/replace=""
conflict 558
/citation=[1]
/replace=""
conflict 561
/citation=[1]
/replace=""
conflict 622..624
/citation=[1]
/replace=""
conflict 642
/citation=[1]
/replace=""
conflict 665..666
/citation=[1]
/replace=""
misc_binding 673..722
/note="FLP recombinase binding site A [9]"
/bound_moiety="FLP recombinase"
misc_recomb 702..703
conflict 793..794
/citation=[1]
/replace=""
mRNA complement(836..2038)
/note="Rep1 mRNA (alt.)"
mRNA complement(836..2019)
/note="Rep1 mRNA (alt.)"
mRNA complement(836..2017)
/note="Rep1 mRNA (alt.)"
mRNA complement(836..2010)
/note="Rep1 mRNA (alt.)"
mRNA complement(836..2005)
/note="Rep1 mRNA (alt.)"
mRNA complement(836..2004)
/note="Rep1 mRNA (alt.)"
CDS complement(887..2008)
/codon_start=1
/product="Rep 1 protein"
/protein_id="AAB59341.1"
/db_xref="GI:172192"
/translation="MNGERLLACIKQCIMQHFQPMVYDESRCVIETTRGTFPVPDNYK
KYKTLAFAFVGHVLNTDDTPVIEKELDWPDPALVYNTIVDRIINHPELSQFISVAFIS
QLKATIGEGLDINVKGTLNRRGKGIRRPKGVFFRYMESPFVNTKVTAFFSYLRDYNKI
ASEYHNNTKFILTFSCQAYWASGPNFSALKNVIRCSIIHEYISKFVEREQDKGHIGDQ
ELPPEEDPSRELNNVQHEVNSLTEQDAEADEGLWGEIDSLCEKWQSEAEDQTEAEIIA
DRIIGNSQRMANLKIRRTKFKSVLYHILKELIQSQGTVKVYRGSSFSHDSIKISLHYE
EQHITAVWVYLTVKFEEHWKPVDVEVEFRCKFKERKVDG"
mRNA 2254..2861
/note="D mRNA (alt.; 5' end +/- 3 bp)"
mRNA 2254..2841
/note="D mRNA (alt.; 5' end +/- 3 bp)"
CDS 2271..2816
/codon_start=1
/product="D protein"
/protein_id="AAB59342.1"
/db_xref="GI:172193"
/translation="MPYKTAIDCIEELATQCFLSKLTDDDVSTFRRVCSKENDIIKLA
LRIPRTIDYTSILRLLYDTLPLRSLSFNEALPLFCYSIDPAQQRQCDLRFYLRDVVKL
ARPRKRLEMQKALLQWLPSLLSDVTLQLLNDIRIRFEEIQPNIRQTVLQIYDRTCYPS
LNFEHPNLGVFPETDSIFEPV"
repeat_region 3714..4312
/note="IR1"
misc_binding 3930..3979
/note="FLP-recombinase binding site B [9]"
/bound_moiety="FLP recombinase"
misc_recomb 3941..3942
mRNA complement(4108..5223)
/note="REP2 mRNA (minor alt.)"
mRNA complement(4108..5212)
/note="REP2 mRNA (minor alt.)"
mRNA complement(4108..5198)
/note="REP2 mRNA (major alt.)"
mRNA complement(4108..5197)
/note="REP2 mRNA (major alt.)"
mRNA complement(4108..5196)
/note="REP2 mRNA (major alt.)"
mRNA complement(4108..5195)
/note="REP2 mRNA (major alt.)"
mRNA complement(4108..5184)
/note="REP2 mRNA (major alt.)"
mRNA complement(4108..5183)
/note="REP2 mRNA (major alt.)"
mRNA complement(4108..5182)
/note="REP2 mRNA (major alt.)"
CDS complement(4308..5198)
/codon_start=1
/product="Rep 2 protein"
/protein_id="AAB59343.1"
/db_xref="GI:172194"
/translation="MDDIETAKNLTVKARTAYSVWDVCRLFIEMIAPDVDIDIESKRK
SDELLFPGYVIRPMESLTTGRPYGLDSSAEDSSVSSDSSAEVILPAAKMVKERFDSIG
NGMLSSQEASQAAIDLMLQNNKLLDNRKQLYKSIAIIIGRLPEKDKKRATEMLMRKMD
CTQLLVPPAPTEEDVMKLVSVVTQLLTLVPPDRQAALIGDLFIPESLKDIFNSFNELA
AENRLQQKKSELEGRTEVNHANTNEEVPSRRTRSRDTNARGAYKLQNTITEGPKAVPT
KKRRVATRVRGRKSRNTSRV"
exon 5549..6318
old_sequence 5583
/citation=[2]
ORIGIN
1 gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca
61 ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa
121 tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt
181 gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg
241 gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat
301 acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga
361 aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct
421 aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga
481 ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata
541 aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg
601 tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt
661 tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac
721 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac
781 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca
841 aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct
901 ttcgctcctt gaacttgcat ctaaactcga cctctacatc aacaggcttc caatgctctt
961 caaattttac tgtcaagtag acccatacgg ctgtaatatg ctgctcttca taatgtaagc
1021 ttatctttat cgaatcgtgt gaaaaactac taccgcgata aacctttacg gttccctgag
1081 attgaattag ttcctttagt atatgataca agacactttt gaactttgta cgacgaattt
1141 tgaggttcgc catcctctgg ctatttccaa ttatcctgtc ggctattatc tccgcctcag
1201 tttgatcttc cgcttcagac tgccattttt cacataatga atctatttca ccccacaatc
1261 cttcatccgc ctccgcatct tgttccgtta aactattgac ttcatgttgt acattgttta
1321 gttcacgaga agggtcctct tcaggcggta gctcctgatc tcctatatga cctttatcct
1381 gttctctttc cacaaactta gaaatgtatt catgaattat ggagcaccta ataacattct
1441 tcaaggcgga gaagtttggg ccagatgccc aatatgcttg acatgaaaac gtgagaatga
1501 atttagtatt attgtgatat tctgaggcaa ttttattata atctcgaaga taagagaaga
1561 atgcagtgac ctttgtattg acaaatggag attccatgta tctaaaaaat acgcctttag
1621 gccttctgat accctttccc ctgcggttta gcgtgccttt tacattaata tctaaaccct
1681 ctccgatggt ggcctttaac tgactaataa atgcaaccga tataaactgt gataattctg
1741 ggtgatttat gattcgatcg acaattgtat tgtacactag tgcaggatca ggccaatcca
1801 gttctttttc aattaccggt gtgtcgtctg tattcagtac atgtccaaca aatgcaaatg
1861 ctaacgtttt gtatttctta taattgtcag gaactggaaa agtccccctt gtcgtctcga
1921 ttacacacct actttcatcg tacaccatag gttggaagtg ctgcataata cattgcttaa
1981 tacaagcaag cagtctctcg ccattcatat ttcagttatt ttccattaca gctgatgtca
2041 ttgtatatca gcgctgtaaa aatctatctg ttacagaagg ttttcgcggt ttttataaac
2101 aaaactttcg ttacgaaatc gagcaatcac cccagctgcg tatttggaaa ttcgggaaaa
2161 agtagagcaa cgcgagttgc attttttaca ccataatgca tgattaactt cgagaaggga
2221 ttaaggctaa tttcactagt atgtttcaaa aacctcaatc tgtccattga atgccttata
2281 aaacagctat agattgcata gaagagttag ctactcaatg ctttttgtca aagcttactg
2341 atgatgatgt gtctactttc aggcgggtct gtagtaagga gaatgacatt ataaagctgg
2401 cacttagaat tccacggact atagactata ctagtatact ccgtctactg tacgatacac
2461 ttccgctcag gtccttgtcc tttaacgagg ccttaccact cttttgttac tctattgatc
2521 cagctcagca aaggcagtgt gatctaagat tctatcttcg cgatgtagta aaactagcta
2581 gaccgagaaa gagactagaa atgcaaaagg cacttctaca atggctgcca tcattattat
2641 ccgatgtgac gctgcagctt ctcaatgata ttcgaatacg ctttgaggag atacagccta
2701 atatccgaca aactgtttta cagatttacg atcgtacttg ttacccatca ttgaattttg
2761 aacatccgaa cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat
2821 atatagtcta gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg
2881 catctattgc ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc
2941 ttgcacttca atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa
3001 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
3061 agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa
3121 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
3181 agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt
3241 gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt
3301 ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg
3361 ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact
3421 ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg
3481 catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat
3541 agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta
3601 tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag
3661 ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga
3721 ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata
3781 tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg
3841 caatatttta gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc
3901 agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac
3961 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
4021 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
4081 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
4141 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
4201 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
4261 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca taccctagaa
4321 gtattacgtg attttctgcc ccttaccctc gttgctactc tccttttttt cgtgggaacc
4381 gctttagggc cctcagtgat ggtgttttgt aatttatatg ctcctcttgc atttgtgtct
4441 ctacttcttg ttcgcctgga gggaacttct tcatttgtat tagcatggtt cacttcagtc
4501 cttccttcca actcactctt tttttgctgt aaacgattct ctgccgccag ttcattgaaa
4561 ctattgaata tatcctttag agattccggg atgaataaat cacctattaa agcagcttga
4621 cgatctggtg gaactaaagt aagcaattgg gtaacgacgc ttacgagctt cataacatct
4681 tcttccgttg gagctggtgg gactaataac tgtgtacaat ccatttttct catgagcatt
4741 tcggtagctc tcttcttgtc tttctcgggc aatcttccta ttattatagc aatagatttg
4801 tatagttgct ttctattgtc taacagcttg ttattctgta gcatcaaatc tatggcagcc
4861 tgacttgctt cttgtgaaga gagcatacca tttccaatcg aatcaaacct ttccttaacc
4921 atcttcgcag caggcaaaat tacctcagca ctggagtcag aagatacgct ggaatcttct
4981 gcgctagaat caagaccata cggcctaccg gttgtgagag attccatggg ccttatgaca
5041 tatcctggaa agagtagctc atcagactta cgtttactct ctatatcaat atctacatca
5101 ggagcaatca tttcaataaa cagccgacat acatcccaga cgctataagc tgtacgtgct
5161 tttaccgtca gattcttggc tgtttcaatg tcgtccattt tggttttctt ttaccagtat
5221 tgttcgtttg ataatgtatt cttgcttatt acattataaa atctgtgcag atcacatgtc
5281 aaaacaactt tttatcacaa gatagtaccg caaaacgaac ctgcgggccg tctaaaaatt
5341 aaggaaaagc agcaaaggtg catttttaaa atatgaaatg aagataccgc agtaccaatt
5401 attttcgcag tacaaataat gcgcggccgg tgcatttttc gaaagaacgc gagacaaaca
5461 ggacaattaa agttagtttt tcgagttagc gtgtttgaat actgcaagat acaagataaa
5521 tagagtagtt gaaactagat atcaattgca cacaagatcg gcgctaagca tgccacaatt
5581 tggtatatta tgtaaaacac cacctaaggt gcttgttcgt cagtttgtgg aaaggtttga
5641 aagaccttca ggtgagaaaa tagcattatg tgctgctgaa ctaacctatt tatgttggat
5701 gattacacat aacggaacag caatcaagag agccacattc atgagctata atactatcat
5761 aagcaattcg ctgagtttcg atattgtcaa taaatcactc cagtttaaat acaagacgca
5821 aaaagcaaca attctggaag cctcattaaa gaaattgatt cctgcttggg aatttacaat
5881 tattccttac tatggacaaa aacatcaatc tgatatcact gatattgtaa gtagtttgca
5941 attacagttc gaatcatcgg aagaagcaga taagggaaat agccacagta aaaaaatgct
6001 taaagcactt ctaagtgagg gtgaaagcat ctgggagatc actgagaaaa tactaaattc
6061 gtttgagtat acttcgagat ttacaaaaac aaaaacttta taccaattcc tcttcctagc
6121 tactttcatc aattgtggaa gattcagcga tattaagaac gttgatccga aatcatttaa
6181 attagtccaa aataagtatc tgggagtaat aatccagtgt ttagtgacag agacaaagac
6241 aagcgttagt aggcacatat acttctttag cgcaaggggt aggatcgatc cacttgtata
6301 tttggatgaa tttttgag
//