NAME

CLIPSeqTools::App::distribution_on_genic_elements - Measure read distribution on 5'UTR, CDS and 3'UTR.

SYNOPSIS

clipseqtools distribution_on_genic_elements [options/parameters]

DESCRIPTION

Measure the distribution of reads along 5'UTR, CDS and 3'UTR. Will split the 5'UTR, CDS and 3'UTR of coding transcripts in bins and measure the read density in each bin. It will randomly keep a single transcript for each gene. The read copy number is not used in this analysis.

OPTIONS

  Input options for library.
    --driver <Str>         driver for database connection (eg. mysql,
                           SQLite).
    --database <Str>       database name or path to database file for file
                           based databases (eg. SQLite).
    --table <Str>          database table.
    --host <Str>           hostname for database connection.
    --user <Str>           username for database connection.
    --password <Str>       password for database connection.
    --records_class <Str>  type of records stored in database.
    --filter <Filter>      filter library. May be used multiple times.
                           Syntax: column_name="pattern"
                           e.g. keep reads with deletions AND not repeat
                                masked AND longer than 31
                                --filter deletion="def"
                                --filter rmsk="undef" .
                                --filter query_length=">31".
                           Operators: >, >=, <, <=, =, !=, def, undef

  Other input
    --gtf <Str>            GTF file with genes/transcripts.

  Output
    --o_prefix <Str>       output path prefix. Script will create and add
                           extension to path. [Default: ./]

  Other options.
    --bins <Int>           number of bins each element is split into.
                           [Default: 10]
    --length_thres <Int>   genic elements shorter than this are skipped.
                           [Default: 300]
    --plot                 call plotting script to create plots.
    -v --verbose           print progress lines and extra information.
    -h -? --usage --help   print help message