computePI.pl - Compute isoelectric point (pI)
./computePI.pl [--method=METHOD] [--current=CURRENT] [--saveparam=PARAM] | [--readparam=PARAM] [--calibratefrom=CAL] [--settings=SETTINGS] [--out=OUT] (filein1 [ filein2[...]] | - )
Algorithm : iterative (default) or Patrickios (approximated).
Coefficients used for the iterative algorithm. Currently available are: Lehninger (default), EMBOSS, Rodwell, Sillero, Solomon.
Output text file (or STDOUT if no such argument is given)
Calibrate the algorithm by fitting some experimental data. File format of blank-separated columns: "amino acid sequences" "isoelectric point" "additional cols" ...
Save current params in PARAM, including calibration.
Retrieve current params in PARAM, including calibration.
XML file with user-supplied additional coefficients. See 'piset.dtd'.
Print a brief help message and exits.
Print the manual page and exits.
All remaining arguments are names of input files; or '-' for the standard input.
This program will estimate the isoelectric point (pI) for a given list of peptides. The point is computed by an iterative algorithm or by a theoretical approximation using the regressed dissociation constants (Patrickios et al, Anal. BioChem. 231, 82-91, 1995).
To install InSilicoSpectro, copy and paste the appropriate command in to your terminal.
cpanm
cpanm InSilicoSpectro
CPAN shell
perl -MCPAN -e shell install InSilicoSpectro
For more information on module installation, please visit the detailed CPAN module installation guide.