Bio::Restriction::IO - Handler for sequence variation IO Formats
use Bio::Restriction::IO; $in = Bio::Restriction::IO->new(-file => "inputfilename" , -format => 'withrefm'); my $res = $in->read; # a Bio::Restriction::EnzymeCollection
Bio::Restriction::IO is a handler module for the formats in the Restriction IO set, e.g.
Bio::Restriction::IO::xxx. It is the officially sanctioned way of getting at the format objects, which most people should use.
The structure, conventions and most of the code is inherited from Bio::SeqIO. The main difference is that instead of using methods
next_seq, you drop
_seq from the method name.
For more details, see documentation in Bio::SeqIO.
At the moment, these can be use mainly to get a custom set if enzymes in
itype2 formats into Bio::Restriction::Enzyme or Bio::Restriction::EnzymeCollection objects. Using
bairoch format is highly experimental and is not recommmended at this time.
write() methods are currently not implemented for any format except
write() even with
base format is not recommended as it does not support multicut/multisite enzyme output.
Should additional formats be supported (such as XML)?
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated.
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rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
Rob Edwards, email@example.com
Heikki Lehvaslaiho, heikki-at-bioperl-dot-org
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
Title : new Usage : $stream = Bio::Restriction::IO->new(-file => $filename, -format => 'Format') Function: Returns a new seqstream Returns : A Bio::Restriction::IO::Handler initialised with the appropriate format Args : -file => $filename -format => format -fh => filehandle to attach to
Title : format Usage : $format = $stream->format() Function: Get the restriction format Returns : restriction format Args : none
Title : read Usage : $renzs = $stream->read Function: reads all the restrction enzymes from the stream Returns : a Bio::Restriction::EnzymeCollection object Args :
Title : write Usage : $stream->write($seq) Function: writes the $seq object into the stream Returns : 1 for success and 0 for error Args : Bio::Restriction::EnzymeCollection object
Title : _guess_format Usage : $obj->_guess_format($filename) Function: Example : Returns : guessed format of filename (lower case) Args :