Christopher Fields > BioPerl-1.6.923 > Bio::Matrix::PSM::InstanceSiteI



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Source   Latest Release: BioPerl-1.6.924


Bio::Matrix::PSM::InstanceSiteI - InstanceSite interface, holds an instance of a PSM


  use Bio::Matrix::PSM::InstanceSite;
  #Y ou can get an InstanceSite object either from a file:
  my ($instances,$matrix)=$SomePSMFile->parse_next;
  #or from memory
  my %params=(seq       => 'TATAAT',
              id        => "TATAbox1", 
              accession => 'ENSG00000122304', 
              mid       => 'TB1',
              desc      => 'TATA box, experimentally verified in PRM1 gene',
              relpos    => -35);


Abstract interface to PSM site occurrence (PSM sequence match). InstanceSite objects may be used to describe a PSM (See Bio::Matrix::PSM::SiteMatrix) sequence matches. The usual characteristic of such a match is sequence coordinates, score, sequence and sequence (gene) identifier- accession number or other id. This object inherits from Bio::LocatableSeq (which defines the real sequence) and might hold a SiteMatrix object, used to detect the CRE (cis-regulatory element), or created from this CRE. While the documentation states that the motif id and gene id (accession) combination should be unique, this is not entirely true- there might be more than one occurrence of the same cis-regulatory element in the upstream region of the same gene. Therefore relpos would be the third element to create a really unique combination.


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AUTHOR - Stefan Kirov ^



Bio::Matrix::PSM::SiteMatrix, Bio::Matrix::PSM::Psm, Bio::Matrix::PSM::IO



 Title   : mid
 Usage   : my $mid=$instance->mid;
 Function: Get/Set the motif id
 Throws  :
 Returns : scalar
 Args    : scalar


 Title   : score
 Usage   : my $score=$instance->score;
 Function: Get/Set the score (mismatches) between the instance and the attached (or
            initial) PSM
 Throws  :
 Returns : real number
 Args    : real number


 Title   : start
 Usage   : my $start=$instance->start;
 Function: Get/Set the position of the instance on the sequence used
 Throws  :
 Returns : integer
 Args    : integer


 Title   : relpos
 Usage   : my $seqpos=$instance->relpos;
 Function: Get/Set the relative position of the instance with respect to the transcription start
            site (if known). Can and usually is negative.
 Throws  :
 Returns : integer
 Args    : integer


 Title   : minstance
 Usage   : my $minstance=$misntance->score;
 Function: Get/Set the unique identifier- sequence id/motif id, for example PRM1_TATAbox.
          Not necessarily human readable.
 Throws  :
 Returns : string
 Args    : string
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