Bio::Community::Tools::Summarizer - Create a summary of communities
use Bio::Community::Tools::Summarizer; # Given a metacommunity, merge community members with the same taxonomy, then # group members at the second level of their taxonomy (i.e. phylum level when # using the Greengenes taxonomy), then group members at less than 1% relative # abundance into a single group called 'Other': my $summarizer = Bio::Community::Tools::Summarizer->new( -metacommunity => $meta, -merge_dups => 1, -by_tax_level => 2, -by_rel_ab => ['<', 1], ); my $summarized_meta = $summarizer->get_summary;
Summarize communities in a metacommunity by grouping members based on their taxonomic affiliation first, then by collapsing or removing members with a relative abundance above or below a specified threshold. Summarizing communities should be the last step of any community analysis, because it compresses communities (members, weights, taxonomy, etc.) in a way that cannot be undone.
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The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
Function: Create a new Bio::Community::Tool::Summarizer object Usage : my $summarizer = Bio::Community::Tools::Summarizer->new( -metacommunity => $meta, ); Args : -metacommunity : See metacommunity(). -merge_dups : See merge_dups(). -identify_dups_by: See identify_dups_by(). -by_tax_level : See by_tax_level(). -by_rel_ab : See by_rel_ab(). Returns : a Bio::Community::Tools::Summarizer object
Function: Get/set communities, given as metacommunity, to summarize. Usage : my $meta = $summarizer->metacommunity; Args : A Bio::Community::Meta object Returns : A Bio::Community::Meta object
Function: Merge duplicate community members into a single one. For example, if your community contained several members with a taxonomic lineage of 'Archaea;Euryarchaeota;Halobacteria', all would be merged into a new member with the same taxonomy and the sum of the counts. See the identify_dups_by() method to specify what constitutes duplicates. Note that merging duplicates takes place before grouping by taxonomy level, by_tax_level(). Usage : $summarizer->merge_dups(1); Args : 0 (no) or 1 (yes). Default: 1 Returns : a positive integer
Function: Define what constitute duplicates, i.e. members that have the same desc() or the same taxon(). Usage : $summarizer->identify_dups_by('taxon'); Args : 'desc' or 'taxon'. Default: 'desc' Returns : 'desc' or 'taxon'
Function: Get/set the taxonomic level at which to group community members. When community members have taxonomic information attached, add the relative abundance of all members belonging to the same taxonomic level. The taxonomic level depends on which taxonomy is used. For the Greengenes taxonomy, level 1 represents kingdom, level 2 represents phylum, and so on, until level 7, representing the species level. Members without taxonomic information are grouped together in a Member with the description 'Unknown taxonomy'. Note that summarizing by taxonomy level takes place before grouping by relative abundance, by_rel_ab(). Also, since each community member represents the combination of multiple members, they have to be given a new weight, that is specific to the community they belong to. Usage : $summarizer->by_tax_level(2); Args : a positive integer Returns : a positive integer
Function: Get/set the relative abundance threshold to group members together. Example: You provide a metacommunity containing multiple communities and you specify to group members with a relative abundance less than 1%. If the abundance of member A is less than 1% in all the communities, it is removed from the communities and added as a new member with the desciption 'Other < 1%' along with all other members that are less than 1% in all the communities. Note that when community members are weighted, the 'Other' group also has to be weighted differently for each community. Usage : $summarizer->by_rel_ab('<', 1); Args : * the type of numeric comparison, '<', '<=', '>=', '>' * the relative abundance threshold (in %) Returns : * the type of numeric comparison, '<', '<=', '>=', '>' * the relative abundance threshold (in %)
Function: Summarize the communities and return an arrayref of fresh communities. Usage : my $summary = $summarizer->get_summary; Args : None Returns : A Bio::Community::Meta object