The Chado schema (http://gmod.org/Chado) is a comprehensive database schema developed largely by developers at UC Berkeley and Harvard working on FlyBase. It is intended to be a generic database schema for model organism use. Its use with GBrowse is supported via a limited implementation of the Das interface from BioPerl. It is limited in that it implements only parts of Bio::DasI interface that is required to make GBrowse work.
The Chado adaptor works through three perl modules that are part of a separate distribution that can be obtained from CPAN:
cpan> install Bio::DB::Das::Chado
In addition to the standard Chado schema, this adaptor requires a few additional views and functions. These are found in two files in the Chado CVS or in a gmod distribution. These are:
It is currently included in the default Chado schema that can be obtained as part of the gmod distribution.
If you already have a Chado instance and want to add these items, the easiest way to do that is to cat the files to stdout and pipe that to a psql command:
% cat sequence-gff-views.sql | psql <Chado-database-name> % cat sequence-gff-funcs.pgsql | psql <Chado-database-name>
A sample Chado configuration file is included in contrib/conf_files/. Since Chado uses the Sequence Ontology for its controlled vocabulary, it is quite likely that this configuration file should work for any instance of Chado once the database-specific parameters are set. Also, depending on what the "reference type" is (usually something like 'chromosome' or 'contig'), the line in the configuration for reference class will need to be modified to agree with your data.
After the tables are created, the user that is running Apache must be granted privileges to select on several tables. Usually that user is 'nobody', although on RedHat systems using RPM installed Apache the user is 'apache'. First create that user in Postgres, then in the psql shell grant select permissions:
CREATE USER nobody; GRANT SELECT ON feature_synonym TO nobody; GRANT SELECT ON synonym TO nobody; GRANT SELECT ON feature_dbxref TO nobody; GRANT SELECT ON dbxref TO nobody; GRANT SELECT ON feature TO nobody; GRANT SELECT ON featureloc TO nobody; GRANT SELECT ON cvterm TO nobody; GRANT SELECT ON feature_relationship TO nobody; GRANT SELECT ON cv TO nobody; GRANT SELECT ON feature_cvterm TO nobody; GRANT SELECT ON featureprop TO nobody; GRANT SELECT ON pub TO nobody; GRANT SELECT ON feature_pub TO nobody; GRANT SELECT ON db TO nobody;
The GBrowse configuration file for a Chado database is the same format as for any other data source, but there are a few notes specific to Chado for GBrowse configuration files. A sample configuration file called 07.Chado.conf is included in the contrib/conf_files directory of this distribution, and is installed in $HTDOCS/gbrowse/contrib/conf_files.
Two items specific to Chado that must go into the configuration file:
The reference class in configuration file must be the Sequence Ontology- Feature Annotation (SOFA) type that is the feature type in Chado that is the foundation type, like 'chromosome', 'region' or 'contig', the the other features in the database are on.
Aggregators must not be used with the Chado adaptor, as they are not needed and do not make sense in this context. They are used in Bio::DB::GFF to construct complex biological objects out of the flat data in GFF files, for example, attaching exons to their mRNA. In Chado, this is not necessary since the relationship between features is clearly defined in the feature_relationship table, and that information is automatically obtained by the Chado adaptor.
Once you are properly configured you should be able to use GBrowse with an URL like http://localhost/cgi-bin/gbrowse/chado/.
If you encounter any bugs or problems with this Chado adaptor, please contact the gmod-schema or gmod-gbrowse mailing lists (http://sourceforge.net/mail/?group_id=27707).
Scott Cain email@example.com 2009/05/08